protein coding gene - cdc20 (SPBC25H2.13c) - DNA polymerase epsilon catalytic subunit Pol2

Gene summary

Standard name

cdc20

Systematic ID

SPBC25H2.13c

Product

DNA polymerase epsilon catalytic subunit Pol2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

pol2

UniProt ID

P87154

ORFeome ID

41/41B02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 3253722..3260706 forward strand

Annotation

Protein features

Orthologs

• POLE (HGNC:9177) Homo sapiens
• cdc20 (SJAG_02908) Schizosaccharomyces japonicus
• POL2 (YNL262W) Saccharomyces cerevisiae

References / Literature

PMID:9356477 - Schizosaccharomyces pombe cdc20+ encodes DNA polymerase epsilon and is required for chromosomal replication but not for the S phase checkpoint.

D'Urso G et al. Proc Natl Acad Sci U S A 1997 Nov 11;94(23):12491-6

PMID:18667534 - Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.

Yin L et al. Mol Biol Cell 2008 Oct;19(10):4374-82

PMID:10888871 - Mitotic replication initiation proteins are not required for pre-meiotic S phase.

Forsburg SL et al. Nat Genet 2000 Jul;25(3):263-8

PMID:19109429 - GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.

Pai CC et al. Mol Biol Cell 2009 Feb;20(4):1213-22

PMID:28481910 - PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.

Daigaku Y et al. PLoS Genet 2017 May;13(5):e1006789

GO_REF:0000002 - Comments

PMID:12697806 - Replication proteins influence the maintenance of telomere length and telomerase protein stability.

Dahlén M et al. Mol Cell Biol 2003 May;23(9):3031-42

PMID:25414009 - Fission yeast RNA triphosphatase reads an Spt5 CTD code.

Doamekpor SK et al. RNA 2015 Jan;21(1):113-23

PMID:34228709 - Expression of the cancer-associated DNA polymerase ε P286R in fission yeast leads to translesion synthesis polymerase dependent hypermutation and defective DNA replication.

Soriano I et al. PLoS Genet 2021 Jul;17(7):e1009526

PMID:10559981 - A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.

Edwards RJ et al. Nat Cell Biol 1999 Nov;1(7):393-8

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:9605404 - DNA polymerase epsilon encoded by cdc20+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe.

Sugino A et al. Genes Cells 1998 Feb;3(2):99-110

PMID:9928931 - The S/M checkpoint at 37 degrees C and the recovery of viability of the mutant poldeltats3 require the crb2+/rhp9+ gene in fission yeast.

Grenon M et al. Mol Gen Genet 1999 Jan;260(6):522-34

PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238

PMID:1934126 - Common genes and pathways in the regulation of the mitotic and meiotic cell cycles of Schizosaccharomyces pombe.

Grallert B et al. Curr Genet 1991 Aug;20(3):199-204

PMID:10779336 - Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase.

Tanaka K et al. Mol Cell Biol 2000 May;20(10):3459-69

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:15367656 - Swi1 and Swi3 are components of a replication fork protection complex in fission yeast.

Noguchi E et al. Mol Cell Biol 2004 Oct;24(19):8342-55

PMID:16738311 - Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.

Win TZ et al. Mol Cell Biol 2006 Jun;26(12):4435-47

PMID:23267073 - Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex.

Gonzalez M et al. Proc Natl Acad Sci U S A 2013 Jan 08;110(2):606-11

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:15388803 - Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast.

Spiga MG et al. Nucleic Acids Res 2004;32(16):4945-53

PMID:36626373 - Using canavanine resistance to measure mutation rates in Schizosaccharomyces pombe.

Pai CC et al. PLoS One 2023;18(1):e0271016

PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.

Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24

PMID:7796804 - A pre-start checkpoint preventing mitosis in fission yeast acts independently of p34cdc2 tyrosine phosphorylation.

Hayles J et al. EMBO J 1995 Jun 15;14(12):2760-71

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.

Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20

PMID:9679144 - Regulation of cell polarity by microtubules in fission yeast.

Sawin KE et al. J Cell Biol 1998 Jul 27;142(2):457-71

PMID:7700230 - DNA polymerase delta is required for the replication feedback control of cell cycle progression in Schizosaccharomyces pombe.

Francesconi S et al. Mol Gen Genet 1995 Mar 10;246(5):561-9

PMID:9693370 - Mutational effect of fission yeast polalpha on cell cycle events.

Bhaumik D et al. Mol Biol Cell 1998 Aug;9(8):2107-23

PMID:28366744 - Fission Yeast Apc15 Stabilizes MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.

May KM et al. Curr Biol 2017 Apr 24;27(8):1221-1228

GO_REF:0000033 - Annotation inferences using phylogenetic trees

GO_REF:0000108 - Automatic assignment of GO terms using logical inference, based on on inter-ontology links.

PMID:10388806 - Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes.

Snaith HA et al. Genetics 1999 Jul;152(3):839-51

PMID:26041456 - RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends.

Audry J et al. EMBO J 2015 Jul 14;34(14):1942-58

PMID:21493688 - Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation.

Guarino E et al. Nucleic Acids Res 2011 Aug;39(14):5978-90

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:28366743 - Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex.

Sewart K et al. Curr Biol 2017 Apr 24;27(8):1213-1220

PMID:23263988 - Quantitative analysis of chromosome condensation in fission yeast.

Petrova B et al. Mol Cell Biol 2013 Mar;33(5):984-98

PMID:11416129 - Schizosaccharomyces pombe cells lacking the amino-terminal catalytic domains of DNA polymerase epsilon are viable but require the DNA damage checkpoint control.

Feng W et al. Mol Cell Biol 2001 Jul;21(14):4495-504

PMID:10757807 - Cid1, a fission yeast protein required for S-M checkpoint control when DNA polymerase delta or epsilon is inactivated.

Wang SW et al. Mol Cell Biol 2000 May;20(9):3234-44

PMID:21099360 - Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.

Matsumoto S et al. Cell Cycle 2010 Dec 01;9(23):4627-37

PMID:18331722 - A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast.

Kimata Y et al. Dev Cell 2008 Mar;14(3):446-54

PMID:9891047 - Mutator phenotype induced by aberrant replication.

Liu VF et al. Mol Cell Biol 1999 Feb;19(2):1126-35

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:26436826 - Polymerase δ replicates both strands after homologous recombination-dependent fork restart.

Miyabe I et al. Nat Struct Mol Biol 2015 Nov;22(11):932-8

PMID:22144917 - The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved.

Miyabe I et al. PLoS Genet 2011 Dec;7(12):e1002407

PMID:11313455 - Fission yeast Rad17 associates with chromatin in response to aberrant genomic structures.

Kai M et al. Mol Cell Biol 2001 May;21(10):3289-301

PMID:25883047 - Genetic and structural analysis of the essential fission yeast RNA polymerase II CTD phosphatase Fcp1.

Schwer B et al. RNA 2015 Jun;21(6):1135-46

PMID:9891039 - Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion.

Tanaka H et al. Mol Cell Biol 1999 Feb;19(2):1038-48

PMID:19205745 - Redundant roles of Srs2 helicase and replication checkpoint in survival and rDNA maintenance in Schizosaccharomyces pombe.

Yasuhira S Mol Genet Genomics 2009 May;281(5):497-509

PMID:29109278 - Coordinated regulation of heterochromatin inheritance by Dpb3-Dpb4 complex.

He H et al. Proc Natl Acad Sci U S A 2017 Nov 21;114(47):12524-12529

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:32355220 - DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.

Onaka AT et al. Commun Biol 2020 Apr 30;3(1):202

PMID:9154809 - A novel mutant allele of Schizosaccharomyces pombe rad26 defective in monitoring S-phase progression to prevent premature mitosis.

Uchiyama M et al. Mol Cell Biol 1997 Jun;17(6):3103-15

PMID:21725325 - Coordination of DNA replication and histone modification by the Rik1-Dos2 complex.

Li F et al. Nature 2011 Jul 03;475(7355):244-8

PMID:9407031 - Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1.

Saka Y et al. Genes Dev 1997 Dec 15;11(24):3387-400

PMID:23349636 - Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.

Roseaulin LC et al. PLoS Genet 2013;9(1):e1003213

PMID:9135148 - p56(chk1) protein kinase is required for the DNA replication checkpoint at 37 degrees C in fission yeast.

Francesconi S et al. EMBO J 1997 Mar 17;16(6):1332-41

PMID:26251183 - POLE mutations in families predisposed to cutaneous melanoma.

Aoude LG et al. Fam Cancer 2015 Dec;14(4):621-8

PMID:6943408 - Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe.

Nasmyth K et al. Mol Gen Genet 1981;182(1):119-24

PMID:22433840 - Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.

Kanke M et al. EMBO J 2012 May 02;31(9):2182-94

PMID:2406029 - Mutation of fission yeast cell cycle control genes abolishes dependence of mitosis on DNA replication.

Enoch T et al. Cell 1990 Feb 23;60(4):665-73

PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.

Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14

PMID:29092815 - The kinase domain residue serine 173 of S chizosaccharomyce s pombe Chk1 kinase is critical for the response to DNA replication stress.

Coulton N et al. Biol Open 2017 Dec 15;6(12):1840-1850

PMID:26368543 - Critical Function of γH2A in S-Phase.

Mejia-Ramirez E et al. PLoS Genet 2015 Sep;11(9):e1005517

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:8521500 - p25rum1 orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase.

Correa-Bordes J et al. Cell 1995 Dec 15;83(6):1001-9

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:8876193 - Microtubules mediate mitochondrial distribution in fission yeast.

Yaffe MP et al. Proc Natl Acad Sci U S A 1996 Oct 15;93(21):11664-8

PMID:14739927 - Regulation of checkpoint kinases through dynamic interaction with Crb2.

Mochida S et al. EMBO J 2004 Jan 28;23(2):418-28

PMID:16990792 - Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.

Yabuuchi H et al. EMBO J 2006 Oct 04;25(19):4663-74

PMID:17112379 - The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.

Locovei AM et al. Cell Div 2006 Nov 17;1:27

PMID:25664722 - A global profile of replicative polymerase usage.

Daigaku Y et al. Nat Struct Mol Biol 2015 Mar;22(3):192-198

PMID:10683155 - A novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints.

Griffiths D et al. J Cell Sci 2000 Mar;113 ( Pt 6):1075-88