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protein coding gene - mis19 (SPBC27B12.02) - kinetochore protein Mis19/Eic1

Gene summary

Standard name
mis19
Systematic ID
SPBC27B12.02
Product
kinetochore protein Mis19/Eic1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
eic1, SPBC30B4.10, kis1
UniProt ID
O42995
ORFeome ID
01/01H03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1323731..1324374 forward strand

Annotation

GO biological process

GO:0034080 - CENP-A containing chromatin assembly

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GO cellular component

GO:0098654 - CENP-A recruiting complex

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GO:0034506 - chromosome, centromeric core domain

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GO:0000776 - kinetochore

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GO:0005654 - nucleoplasm

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GO molecular function

GO:0140463 - chromatin-protein adaptor activity

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GO:0005515 - protein binding

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Multi-locus phenotype

FYPO:0004396 - normal mitotic spindle elongation

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003304 - abnormal mitotic spindle midzone assembly

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Genotypes:

FYPO:0003786 - abolished protein localization to centromere central core during vegetative growth

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Genotypes:

FYPO:0000634 - abolished protein localization to centromere during vegetative growth

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0001270 - complete but unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0005215 - decreased protein localization to kinetochore during mitotic M phase

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Genotypes:

FYPO:0002902 - decreased protein localization to kinetochore during vegetative growth

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002638 - increased activation of mitotic spindle assembly checkpoint

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Genotypes:

FYPO:0002148 - inviable after spore germination, multiple cell divisions, abnormal morphology

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Genotypes:

FYPO:0001489 - inviable vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0006172 - mitotic spindle collapse without elongation during anaphase B

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Genotypes:

Taxonomic conservation

PBO:0016885 - Schizosaccharomyces specific

Warnings

PBO:0000070 - gene structure updated

References:

PBO:0000199 - previously annotated as dubious

References / Literature

PMID:25375240 - The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A.
Hirai H et al. PLoS One 2014;9(11):e111905
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24789708 - Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly.
Subramanian L et al. Open Biol 2014 Apr 30;4(4):140043
PMID:15369671 - Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.
Hayashi T et al. Cell 2004 Sep 17;118(6):715-29
PMID:31371524 - Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex.
Korntner-Vetter M et al. Life Sci Alliance 2019 Aug;2(4)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:35970865 - The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.
Hirai H et al. Commun Biol 2022 Aug 15;5(1):818
PMID:24774534 - Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex.
Hayashi T et al. Genes Cells 2014 Jul;19(7):541-54
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29804820 - Mis16 Switches Function from a Histone H4 Chaperone to a CENP-A Cnp1 -Specific Assembly Factor through Eic1 Interaction.
An S et al. Structure 2018 Jul 03;26(7):960-971.e4