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protein coding gene - erg32 (SPBC27B12.03c) - C-5 sterol desaturase Erg32

Gene summary

Standard name
erg32
Systematic ID
SPBC27B12.03c
Product
C-5 sterol desaturase Erg32
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13666
ORFeome ID
32/32C05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1324193..1325464 reverse strand

Annotation

PBO:0000668 - 1.14.21.6

Disease association

MONDO:0011816 - lathosterolosis

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GO biological process

GO:0006696 - ergosterol biosynthetic process

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GO cellular component

GO:0005783 - endoplasmic reticulum

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GO:0005789 - endoplasmic reticulum membrane

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GO:0005794 - Golgi apparatus

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GO:0000139 - Golgi membrane

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GO molecular function

GO:0000248 - C-5 sterol desaturase activity

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GO:0050046 - delta7-sterol 5(6)-desaturase activity

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GO:0005506 - iron ion binding

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Modification

MOD:00006 - N-glycosylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0010046 - abolished giant endoplasmic reticulum sheet formation during cellular response to saturated fatty acid

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Genotypes:

FYPO:0002128 - abolished protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth

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Genotypes:

FYPO:0000426 - normal endocytosis

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0010042 - normal growth on pentadecanoic acid

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Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

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Genotypes:

FYPO:0002717 - normal vacuole fusion during cellular hypotonic response

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Genotypes:

FYPO:0000070 - resistance to amphotericin B

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Genotypes:

FYPO:0000076 - resistance to nystatin

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Genotypes:

FYPO:0010040 - resistance to pentadecanoic acid

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000113 - sensitive to staurosporine

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Genotypes:

FYPO:0002788 - small vacuoles during vegetative growth

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000070 - resistance to amphotericin B

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000076 - resistance to nystatin

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000113 - sensitive to staurosporine

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04116FA_hydroxylaseFatty_acid_hydroxylasePFAM
PTHR11863STEROL DESATURASESterol_desaturase-relPANTHER

Orthologs

References / Literature

GO_REF:0000002 - Comments
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:17477863 - Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study.
Rustici G et al. Genome Biol 2007;8(5):R73
PMID:19486165 - Pleiotropic phenotypes caused by an opal nonsense mutation in an essential gene encoding HMG-CoA reductase in fission yeast.
Fang Y et al. Genes Cells 2009 Jun;14(6):759-71
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23145048 - A genetic and pharmacological analysis of isoprenoid pathway by LC-MS/MS in fission yeast.
Takami T et al. PLoS One 2012;7(11):e49004
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:17614284 - The chromatin-remodeling factor FACT contributes to centromeric heterochromatin independently of RNAi.
Lejeune E et al. Curr Biol 2007 Jul 17;17(14):1219-24
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:18310029 - Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe.
Iwaki T et al. Microbiology (Reading) 2008 Mar;154(Pt 3):830-841
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:40424131 - Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast.
Hoshikawa Y et al. Proc Natl Acad Sci U S A 2025 Jun 03;122(22):e2422126122