protein coding gene - toa1 (SPBC28F2.09) - transcription factor TFIIA complex large subunit Toa1

Gene summary

Standard name: toa1
Systematic ID: SPBC28F2.09
Product: transcription factor TFIIA complex large subunit Toa1
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: Q9USU9
ORFeome ID: 19/19B02
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome II: 1585210..1586688 forward strand

Annotation

GO biological process

GO:0006367 - transcription initiation at RNA polymerase II promoter

References:

GO_REF:0000024

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO:0005672 - transcription factor TFIIA complex

References:

GO_REF:0000033

GO molecular function

GO:0016251 - RNA polymerase II general transcription initiation factor activity

References:

GO_REF:0000024

Modification

MOD:00006 - N-glycosylated residue

References:

PMID:22633491

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PMID:39367033

Quantitative gene expression

PBO:0006310 - protein level

References:

PMID:23101633

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

PMID:23697806

Genotypes:

toa1delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

toa1delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF03153	TFIIA	TFIIA_asu/bsu	PFAM
cd07976	TFIIA_alpha_beta_like		CDD
SM01371	TFIIA_2	TFIIA_asu/bsu	SMART
G3DSA:2.30.18.10:FF:000008			FUNFAM
G3DSA:1.10.287.100:FF:000001			FUNFAM
SSF50784	Transcription factor IIA (TFIIA), beta-barrel domain	TFIIA_b-brl	SUPERFAMILY
SSF47396	Transcription factor IIA (TFIIA), alpha-helical domain		SUPERFAMILY
G3DSA:1.10.287.100			GENE3D
G3DSA:2.30.18.10		TFIIA_b-brl	GENE3D
PTHR12694	TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1	TFIIA_asu/bsu	PANTHER
mobidb-lite-Disorder	disorder_prediction		MOBIDB-Disorder
mobidb-lite-Negative-Polyelectrolyte	disorder_prediction		MOBIDB-Negative-Polyelectrolyte
mobidb-lite-Polar	disorder_prediction		MOBIDB-Polar

Orthologs

GTF2A1L (HGNC:30727) Homo sapiens
GTF2A1 (HGNC:4646) Homo sapiens
toa1 (SJAG_01710) Schizosaccharomyces japonicus
TOA1 (YOR194C) Saccharomyces cerevisiae

References / Literature

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.: Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.: Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.: Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.: Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:22540037 - Predicting the fission yeast protein interaction network.: Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.: Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.: Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.: Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.: Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;