protein coding gene - exo1 (SPBC29A10.05) - exonuclease I Exo1

Gene summary

Standard name

exo1

Systematic ID

SPBC29A10.05

Product

exonuclease I Exo1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

mut2

UniProt ID

P53695

ORFeome ID

51/51E02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 2545488..2547971 forward strand

Annotation

Protein features

Orthologs

• EXO1 (HGNC:3511) Homo sapiens
• exo1 (SJAG_01832) Schizosaccharomyces japonicus
• DIN7 (YDR263C) Saccharomyces cerevisiae
• EXO1 (YOR033C) Saccharomyces cerevisiae

References / Literature

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:19798055 - Failed gene conversion leads to extensive end processing and chromosomal rearrangements in fission yeast.

Tinline-Purvis H et al. EMBO J 2009 Nov 04;28(21):3400-12

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:31563844 - The roles of fission yeast exonuclease 5 in nuclear and mitochondrial genome stability.

Sparks JL et al. DNA Repair (Amst) 2019 Nov;83:102720

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:25203555 - Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2.

Zhang JM et al. PLoS Biol 2014 Sep;12(9):e1001946

PMID:17936710 - Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination.

Limbo O et al. Mol Cell 2007 Oct 12;28(1):134-46

PMID:21123655 - Break-induced ATR and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast.

Moss J et al. Genes Dev 2010 Dec 01;24(23):2705-16

PMID:27098497 - CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection.

Zeng M et al. Nat Commun 2016 Apr 21;7:11364

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:29774234 - The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication.

Matmati S et al. Sci Adv 2018 May;4(5):eaar2740

PMID:29215009 - The end-joining factor Ku acts in the end-resection of double strand break-free arrested replication forks.

Teixeira-Silva A et al. Nat Commun 2017 Dec 07;8(1):1982

PMID:19470480 - Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.

Farah JA et al. Proc Natl Acad Sci U S A 2009 Jun 09;106(23):9356-61

PMID:19752195 - Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.

Milman N et al. Mol Cell Biol 2009 Nov;29(22):5998-6005

PMID:25580577 - Tetrameric Ctp1 coordinates DNA binding and DNA bridging in DNA double-strand-break repair.

Andres SN et al. Nat Struct Mol Biol 2015 Feb;22(2):158-66

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.

Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650

PMID:23080121 - Mre11 ATLD17/18 mutation retains Tel1/ATM activity but blocks DNA double-strand break repair.

Limbo O et al. Nucleic Acids Res 2012 Dec;40(22):11435-49

PMID:32341083 - Active Replication Checkpoint Drives Genome Instability in Fission Yeast mcm4 Mutant.

Kim SM et al. Mol Cell Biol 2020 Jun 29;40(14)

PMID:27325741 - Ctp1-dependent clipping and resection of DNA double-strand breaks by Mre11 endonuclease complex are not genetically separable.

Jensen KL et al. Nucleic Acids Res 2016 Sep 30;44(17):8241-9

PMID:24623809 - The DNA damage checkpoint pathway promotes extensive resection and nucleotide synthesis to facilitate homologous recombination repair and genome stability in fission yeast.

Blaikley EJ et al. Nucleic Acids Res 2014 May;42(9):5644-56

PMID:29697047 - Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks.

Leland BA et al. Elife 2018 Apr 26;7

PMID:21441914 - ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair.

Williams GJ et al. Nat Struct Mol Biol 2011 Apr;18(4):423-31

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:28341698 - Schizosaccharomyces pombe MutSα and MutLα Maintain Stability of Tetra-Nucleotide Repeats and Msh3 of Hepta-Nucleotide Repeats.

Villahermosa D et al. G3 (Bethesda) 2017 May 05;7(5):1463-1473

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:24806966 - The extent of error-prone replication restart by homologous recombination is controlled by Exo1 and checkpoint proteins.

Tsang E et al. J Cell Sci 2014 Jul 01;127(Pt 13):2983-94

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:7855597 - A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction.

Szankasi P et al. Science 1995 Feb 24;267(5201):1166-9

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.

Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55

PMID:26368543 - Critical Function of γH2A in S-Phase.

Mejia-Ramirez E et al. PLoS Genet 2015 Sep;11(9):e1005517

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:26957021 - The RecQ DNA helicase Rqh1 constrains Exonuclease 1-dependent recombination at stalled replication forks.

Osman F et al. Sci Rep 2016 Mar 09;6:22837

PMID:28159842 - Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells.

Liu B et al. J Biol Chem 2017 Mar 24;292(12):4777-4788

PMID:22761595 - The Rad4(TopBP1) ATR-activation domain functions in G1/S phase in a chromatin-dependent manner.

Lin SJ et al. PLoS Genet 2012 Jun;8(6):e1002801

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:15474417 - EXO1-A multi-tasking eukaryotic nuclease.

Tran PT et al. DNA Repair (Amst) 2004 Dec 02;3(12):1549-59

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:25965521 - Ku stabilizes replication forks in the absence of Brc1.

Sánchez A et al. PLoS One 2015;10(5):e0126598

PMID:23297345 - Fission yeast RecQ helicase Rqh1 is required for the maintenance of circular chromosomes.

Nanbu T et al. Mol Cell Biol 2013 Mar;33(6):1175-87

PMID:26465752 - Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres.

Nanbu T et al. PLoS One 2015;10(10):e0140456

PMID:15485922 - Fission yeast Dna2 is required for generation of the telomeric single-strand overhang.

Tomita K et al. Mol Cell Biol 2004 Nov;24(21):9557-67

PMID:29167439 - Eroded telomeres are rearranged in quiescent fission yeast cells through duplications of subtelomeric sequences.

Maestroni L et al. Nat Commun 2017 Nov 22;8(1):1684

PMID:27729451 - Cdc24 Is Essential for Long-range End Resection in the Repair of Double-stranded DNA Breaks.

Zhang H et al. J Biol Chem 2016 Nov 25;291(48):24961-24973

PMID:28586299 - Inter-Fork Strand Annealing causes genomic deletions during the termination of DNA replication.

Morrow CA et al. Elife 2017 Jun 06;6

PMID:24943839 - A role for nuclear envelope-bridging complexes in homology-directed repair.

Swartz RK et al. Mol Biol Cell 2014 Aug 15;25(16):2461-71

PMID:28475874 - Unprotected Replication Forks Are Converted into Mitotic Sister Chromatid Bridges.

Ait Saada A et al. Mol Cell 2017 May 04;66(3):398-410.e4

PMID:28922417 - Lingering single-strand breaks trigger Rad51-independent homology-directed repair of collapsed replication forks in the polynucleotide kinase/phosphatase mutant of fission yeast.

Sanchez A et al. PLoS Genet 2017 Sep;13(9):e1007013

PMID:9136000 - The Schizosaccharomyces pombe rec16 gene product regulates multiple meiotic events.

Li YF et al. Genetics 1997 May;146(1):57-67

PMID:9871115 - Schizosaccharomyces pombe exo1 is involved in the same mismatch repair pathway as msh2 and pms1.

Rudolph C et al. Curr Genet 1998 Dec;34(5):343-50

PMID:12713814 - Different frameshift mutation spectra in non-repetitive DNA of MutSalpha- and MutLalpha-deficient fission yeast cells.

Marti TM et al. DNA Repair (Amst) 2003 May 13;2(5):571-80

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:21931565 - Release of Ku and MRN from DNA ends by Mre11 nuclease activity and Ctp1 is required for homologous recombination repair of double-strand breaks.

Langerak P et al. PLoS Genet 2011 Sep;7(9):e1002271

PMID:18388861 - Mus81 is essential for sister chromatid recombination at broken replication forks.

Roseaulin L et al. EMBO J 2008 May 07;27(9):1378-87

PMID:33159083 - The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal.

Kramarz K et al. Nat Commun 2020 Nov 06;11(1):5643

GO_REF:0000002 - Comments

PMID:17277362 - Brc1-mediated rescue of Smc5/6 deficiency: requirement for multiple nucleases and a novel Rad18 function.

Lee KM et al. Genetics 2007 Apr;175(4):1585-95

PMID:23211746 - Initiation of DNA damage responses through XPG-related nucleases.

Kuntz K et al. EMBO J 2013 Jan 23;32(2):290-302

PMID:18854158 - Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair.

Williams RS et al. Cell 2008 Oct 03;135(1):97-109

PMID:1737756 - A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe.

Szankasi P et al. J Biol Chem 1992 Feb 15;267(5):3014-23

PMID:33723569 - Schizosaccharomyces pombe KAT5 contributes to resection and repair of a DNA double-strand break.

Li T et al. Genetics 2021 May 17;218(1)

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:38718864 - H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe.

Qin B et al. J Biol Chem 2024 May 06;:107345

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:12861005 - Competition between the Rad50 complex and the Ku heterodimer reveals a role for Exo1 in processing double-strand breaks but not telomeres.

Tomita K et al. Mol Cell Biol 2003 Aug;23(15):5186-97

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:11333219 - Control of GT repeat stability in Schizosaccharomyces pombe by mismatch repair factors.

Mansour AA et al. Genetics 2001 May;158(1):77-85

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:23188080 - The fission yeast MRN complex tethers dysfunctional telomeres for NHEJ repair.

Reis CC et al. EMBO J 2012 Dec 12;31(24):4576-86

PMID:17724078 - Break-induced loss of heterozygosity in fission yeast: dual roles for homologous recombination in promoting translocations and preventing de novo telomere addition.

Cullen JK et al. Mol Cell Biol 2007 Nov;27(21):7745-57

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:32784607 - The Chromatin Response to Double-Strand DNA Breaks and Their Repair.

Aleksandrov R et al. Cells 2020 Aug 07;9(8)

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405