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protein coding gene - myo51 (SPBC2D10.14c) - myosin type V

Gene summary

Standard name
myo51
Systematic ID
SPBC2D10.14c
Product
myosin type V
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
myo5
UniProt ID
O74805
ORFeome ID
30/30H10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2990178..2994914 reverse strand

Annotation

Disease association

MONDO:0030810 - cholestasis, progressive familial intrahepatic, 10

References:

MONDO:0008962 - Griscelli syndrome type 1

References:

MONDO:0002561 - lysosomal storage disease

References:

MONDO:0009635 - microvillus inclusion disease

References:

GO biological process

GO:0061572 - actin filament bundle organization

References:

GO:0071520 - actomyosin contractile ring assembly actin filament bundle convergence

References:

GO:0006897 - endocytosis

References:

GO:0070649 - formin-nucleated actin cable assembly

References:

GO:1904600 - mating projection actin fusion focus assembly

References:

GO:0016192 - vesicle-mediated transport

References:

GO cellular component

GO:0032153 - cell division site

References:

GO:0005829 - cytosol

References:

GO:0070648 - formin-nucleated actin cable

References:

GO:1990819 - mating projection actin fusion focus

References:

GO:0031097 - medial cortex

References:

GO:0016020 - membrane

References:

GO:0110085 - mitotic actomyosin contractile ring

References:

GO:0120104 - mitotic actomyosin contractile ring, proximal layer

References:

GO:0031475 - myosin V complex

References:

GO molecular function

GO:0051015 - actin filament binding

References:

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0000146 - microfilament motor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Multi-locus phenotype

FYPO:0000161 - abnormal actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0003013 - abnormal actomyosin contractile ring disassembly

References:

Genotypes:

FYPO:0000032 - abnormal cytokinesis during vegetative growth

References:

Genotypes:

FYPO:0002872 - abnormal endoplasmic reticulum localization

References:

Genotypes:

FYPO:0006497 - abnormal plasma membrane phosphatidylserine distribution

References:

Genotypes:

FYPO:0004802 - abnormal protein localization to shmoo tip

References:

Genotypes:

FYPO:0000413 - abolished cell fusion during mating

References:

Genotypes:

FYPO:0007966 - abolished nuclear displacement from cell division site after abolished mitotic sister chromatid separation

References:

Genotypes:

FYPO:0001585 - abolished protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0007201 - decreased rate of actin cortical patch localization to cell division site

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000729 - delayed onset of actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0004653 - delayed onset of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002401 - microtubule bundles present in increased numbers

References:

Genotypes:

FYPO:0006005 - normal actomyosin contractile ring localization

References:

Genotypes:

FYPO:0007689 - normal cell growth polarization

References:

Genotypes:

FYPO:0007114 - normal microtubule bundle

References:

Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0001945 - normal protein secretion

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0006386 - protein abnormally dispersed at shmoo tip

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

FYPO:0007231 - viable spindle-shaped cell

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0007683 - wide actin fusion focus

References:

Genotypes:

Protein features

PBO:0111829 - IQ domain

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002032 - abnormal actin cable morphology during mitosis

References:

Genotypes:

FYPO:0003444 - abnormal medial cortical node condensation

References:

Genotypes:

FYPO:0006503 - abnormal protein localization to actin fusion focus

References:

Genotypes:

FYPO:0004802 - abnormal protein localization to shmoo tip

References:

Genotypes:

FYPO:0003442 - abolished protein localization to actin cable

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003440 - cell lysis during cytokinesis

References:

Genotypes:

FYPO:0003443 - decreased medial cortical node movement during contractile ring assembly

References:

Genotypes:

FYPO:0002441 - decreased rate of actin cable retrograde transport

References:

Genotypes:

FYPO:0003339 - decreased rate of actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

References:

Genotypes:

FYPO:0004807 - narrow cell fusion site following mating

References:

Genotypes:

FYPO:0002487 - normal actin cable morphology

References:

Genotypes:

FYPO:0001294 - normal actin cortical patch localization

References:

Genotypes:

FYPO:0000426 - normal endocytosis

References:

Genotypes:

FYPO:0002559 - normal protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0002443 - normal rate of actin cable retrograde transport

References:

Genotypes:

FYPO:0004895 - normal rate of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0002253 - normal septum location

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001125 - normal vegetative cell shape

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

FYPO:0007683 - wide actin fusion focus

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00063Myosin_headMyosin_head_motor_dom-likePfam
PF00612IQIQ_motif_EF-hand-BSPfam
PF01843DILDilute_domPfam
cd01380MYSc_Myo5MYSc_Myo5CDD
cd15474Myo5p-like_CBD_fungalCDD
cd23767IQCDCDD
PS50096IQPROSITE profiles
PS51126DILUTEDilute_domPROSITE profiles
PS51456MYOSIN_MOTORMyosin_head_motor_dom-likePROSITE profiles
PS51844SH3_LIKESH3_MyosinPROSITE profiles
SM00015IQIQ_motif_EF-hand-BSSMART
SM00242MYScMyosin_head_motor_dom-likeSMART
SM01132DILDilute_domSMART
PR00193MYOSINHEAVYMyosin_head_motor_dom-likePRINTS
G3DSA:1.10.10.820:FF:000001CATH-FunFam
G3DSA:1.10.10.820CATH-Gene3D
G3DSA:1.20.120.720CATH-Gene3D
G3DSA:1.20.5.190CATH-Gene3D
G3DSA:1.20.58.530CATH-Gene3D
G3DSA:3.40.850.10Kinesin_motor_dom_sfCATH-Gene3D
G3DSA:6.20.240.20CATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR13140PANTHER
CoilCOILS

Orthologs

References / Literature

PMID:11942609 - Interactions of Cdc4p, a myosin light chain, with IQ-domain containing proteins in Schizosaccharomyces pombe.
D'souza VM et al. Cell Struct Funct 2001 Dec;26(6):555-65
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:27432898 - A single-headed fission yeast myosin V transports actin in a tropomyosin-dependent manner.
Tang Q et al. J Cell Biol 2016 Jul 18;214(2):167-79
PMID:30201262 - Fission yeast Adf1 is necessary for reassembly of actin filaments into the contractile ring during cytokinesis.
Ueda EI et al. Biochem Biophys Res Commun 2018 Nov 25;506(2):330-338
PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA et al. Elife 2017 Sep 15;6
PMID:23051734 - Myosin Vs organize actin cables in fission yeast.
Lo Presti L et al. Mol Biol Cell 2012 Dec;23(23):4579-91
PMID:20705471 - Differential regulation of unconventional fission yeast myosins via the actin track.
Clayton JE et al. Curr Biol 2010 Aug 24;20(16):1423-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21148300 - Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.
Bendezú FO et al. Mol Biol Cell 2011 Jan 01;22(1):44-53
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24798735 - The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis.
Wang N et al. J Cell Biol 2014 May 12;205(3):357-75
PMID:24531330 - Getting myosin-V on the right track: tropomyosin sorts transport in yeast.
Pollard LW et al. Bioarchitecture 2014;4(1):35-8
PMID:11359928 - Identification of two type V myosins in fission yeast, one of which functions in polarized cell growth and moves rapidly in the cell.
Motegi F et al. Mol Biol Cell 2001 May;12(5):1367-80
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:25825517 - A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.
Dudin O et al. J Cell Biol 2015 Mar 30;208(7):897-911
PB_REF:0000003 - Disease Association Curation
PMID:33506191 - Escape from mitotic catastrophe by actin-dependent nuclear displacement in fission yeast.
Yukawa M et al. iScience 2021 Jan 22;24(1):102031
PMID:39330407 - The Myosin-V Myo51 and Alpha-Actinin Ain1p Cooperate during Contractile Ring Assembly and Disassembly in Fission Yeast Cytokinesis.
Tyree ZL et al. J Fungi (Basel) 2024 Sep 12;10(9)
PMID:30928696 - Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions.
Okada H et al. iScience 2019 Apr 26;14:69-87
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23041194 - Plasma membrane tethering of the cortical ER necessitates its finely reticulated architecture.
Zhang D et al. Curr Biol 2012 Nov 06;22(21):2048-52
PMID:19805578 - Genome-wide mapping of myosin protein-RNA networks suggests the existence of specialized protein production sites.
Mata J FASEB J 2010 Feb;24(2):479-84
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:11112691 - Two type V myosins with non-overlapping functions in the fission yeast Schizosaccharomyces pombe: Myo52 is concerned with growth polarity and cytokinesis, Myo51 is a component of the cytokinetic actin ring.
Win TZ et al. J Cell Sci 2001 Jan;114(Pt 1):69-79
PMID:28784611 - Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.
Snider CE et al. J Cell Biol 2017 Oct 02;216(10):3041-3050
PMID:36202103 - Condensation of the fusion focus by the intrinsically disordered region of the formin Fus1 is essential for cell-cell fusion.
Billault-Chaumartin I et al. Curr Biol 2022 Nov 07;32(21):4752-4761.e10
PMID:41335110 - High-resolution mapping of the actin fusion focus reveals myosin V-dependent formin transport for aster formation.
Thomas V et al. J Cell Biol 2026 Feb 02;225(2)
PMID:16421926 - A critical role for the type V myosin, Myo52, in septum deposition and cell fission during cytokinesis in Schizosaccharomyces pombe.
Mulvihill DP et al. Cell Motil Cytoskeleton 2006 Mar;63(3):149-61
PMID:22137473 - Shaping fission yeast cells by rerouting actin-based transport on microtubules.
Lo Presti L et al. Curr Biol 2011 Dec 20;21(24):2064-9
PMID:27206859 - TOR complex 2 localises to the cytokinetic actomyosin ring and controls the fidelity of cytokinesis.
Baker K et al. J Cell Sci 2016 Jul 01;129(13):2613-24
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29290560 - ER-PM Contacts Restrict Exocytic Sites for Polarized Morphogenesis.
Ng AYE et al. Curr Biol 2018 Jan 08;28(1):146-153.e5
PMID:27852900 - Gradients of phosphatidylserine contribute to plasma membrane charge localization and cell polarity in fission yeast.
Haupt A et al. Mol Biol Cell 2017 Jan 01;28(1):210-220
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:11509236 - Myosin V-mediated vacuole distribution and fusion in fission yeast.
Mulvihill DP et al. Curr Biol 2001 Jul 24;11(14):1124-7
PMID:31495586 - Capping Protein Insulates Arp2/3-Assembled Actin Patches from Formins.
Billault-Chaumartin I et al. Curr Biol 2019 Oct 07;29(19):3165-3176.e6
GO_REF:0000002 - Comments
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23185032 - Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast.
Huang J et al. J Cell Biol 2012 Nov 26;199(5):831-47
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:19098712 - Rng3, a member of the UCS family of myosin co-chaperones, associates with myosin heavy chains cotranslationally.
Amorim MJ et al. EMBO Rep 2009 Feb;10(2):186-91