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protein coding gene - mhf1 (SPBC2D10.16) - CENP-S ortholog, FANCM-MHF complex subunit Mhf1

Gene summary

Standard name
mhf1
Systematic ID
SPBC2D10.16
Product
CENP-S ortholog, FANCM-MHF complex subunit Mhf1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74807
ORFeome ID
04/04A06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2996345..2997147 forward strand

Annotation

Disease association

MONDO:0019391 - Fanconi anemia

References:

GO biological process

GO:0045003 - double-strand break repair via synthesis-dependent strand annealing

References:

GO:0031297 - replication fork processing

References:

GO:0000712 - resolution of meiotic recombination intermediates

References:

GO cellular component

GO:0061838 - CENP-T-W-S-X complex

References:

GO:0071821 - FANCM-MHF complex

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GO molecular function

GO:0003682 - chromatin binding

References:

GO:0003690 - double-stranded DNA binding

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GO:0005515 - protein binding

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Multi-locus phenotype

FYPO:0006280 - increased mitotic recombination with crossover formation at centromere

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Protein features

PBO:0111763 - histone fold

Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000427 - abnormal G1 to G0 transition

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Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0009075 - decreased cell population growth on fructose carbon source

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Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0001740 - increased gross chromosomal rearrangement

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Genotypes:

FYPO:0002484 - increased intergenic meiotic recombination

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Genotypes:

FYPO:0006280 - increased mitotic recombination with crossover formation at centromere

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0000238 - inviable cell upon G0 to G1 transition

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Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

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Genotypes:

FYPO:0002619 - normal growth on sodium butyrate

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Genotypes:

FYPO:0002620 - normal growth on trichostatin A

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000102 - sensitive to cisplatin

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Genotypes:

FYPO:0001245 - sensitive to cobalt

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0003559 - sensitive to doxorubicin

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0007938 - sensitive to tea tree oil

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002476 - viable vacuolated vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF15630CENP-SCENP-S/Mhf1PFAM
cd22919HFD_CENP-SCDD
SSF47113Histone-foldHistone-foldSUPERFAMILY
G3DSA:1.10.20.10Histone, subunit AHistone-foldGENE3D
PTHR22980CORTISTATINPANTHER

Orthologs

References / Literature

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:26791325 - Predicting chemotherapeutic drug combinations through gene network profiling.
Nguyen TT et al. Sci Rep 2016 Jan 21;6:18658
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:30116786 - Genetic regulation of mitotic competence in G 0 quiescent cells.
Sajiki K et al. Sci Adv 2018 Aug;4(8):eaat5685
PMID:23365689 - Cellular robustness conferred by genetic crosstalk underlies resistance against chemotherapeutic drug doxorubicin in fission yeast.
Tay Z et al. PLoS One 2013;8(1):e55041
PMID:22723423 - The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.
Lorenz A et al. Science 2012 Jun 22;336(6088):1585-8
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25669599 - Fitness profiling links topoisomerase II regulation of centromeric integrity to doxorubicin resistance in fission yeast.
Nguyen TT et al. Sci Rep 2015 Feb 11;5:8400
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.
Jian Y et al. J Cell Sci 2023 Jan 15;136(2)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.
Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20347428 - A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability.
Yan Z et al. Mol Cell 2010 Mar 26;37(6):865-78
PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.
Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:34810257 - Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly.
Dong Q et al. Proc Natl Acad Sci U S A 2021 Nov 30;118(48)
PMID:27255861 - Genetic evidence for involvement of membrane trafficking in the action of 5-fluorouracil.
Hu L et al. Fungal Genet Biol 2016 Aug;93:17-24
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.
Zhang L et al. PLoS One 2013;8(7):e68738