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protein coding gene - ade9 (SPBC2G2.08) - C-1-tetrahydrofolatesynthase/methylenetetrahydrof olatedehydrogenase/methylenetetrahydrofolatecyclohydrolase/ formyltetrahydrofolatesynthetase Ade9

Gene summary

Standard name
ade9
Systematic ID
SPBC2G2.08
Product
C-1-tetrahydrofolatesynthase/methylenetetrahydrof olatedehydrogenase/methylenetetrahydrofolatecyclohydrolase/ formyltetrahydrofolatesynthetase Ade9
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
min6
UniProt ID
O43007
ORFeome ID
47/47H11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3448906..3452766 forward strand

Annotation

PBO:0003924 - 1.5.1.15

PBO:0003277 - 1.5.1.5

PBO:0004513 - 2.3.1.16

PBO:0003275 - 6.3.4.3

Disease association

MONDO:0060611 - combined immunodeficiency and megaloblastic anemia with or without hyperhomocysteinemia

References:

MONDO:0001860 - folic acid deficiency anemia

References:

MONDO:0011120 - neural tube defects, folate-sensitive

References:

GO biological process

GO:0009257 - 10-formyltetrahydrofolate biosynthetic process

References:

GO:0046656 - folic acid biosynthetic process

References:

GO:0035999 - tetrahydrofolate interconversion

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005739 - mitochondrion

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0004329 - formate-tetrahydrofolate ligase activity

References:

GO:0004477 - methenyltetrahydrofolate cyclohydrolase activity

References:

GO:0004488 - methylenetetrahydrofolate dehydrogenase (NADP+) activity

References:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0008128 - decreased vegetative cell population growth on low glucose

References:

Genotypes:

FYPO:0000747 - growth auxotrophic for adenine

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0000741 - increased cellular pigment accumulation

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0002634 - resistance to cobalt

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0001701 - sensitive to bortezomib

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

PBO:0000912 - inferred from genetic map position

Protein features

IDNameInterPro nameDB name
PF00763THF_DHG_CYHTHF_DH/CycHdrlase_cat_domPfam
PF01268FTHFSFormate_THF_ligasePfam
PF02882THF_DHG_CYH_CTHF_DH/CycHdrlase_NAD-bd_domPfam
cd00477FTHFSFormate_THF_ligaseCDD
cd01080NAD_bind_m-THF_DH_CyclohydTHF_DH/CycHdrlase_NAD-bd_domCDD
PS00721FTHFS_1Formate_THF_ligase_CSPROSITE patterns
PS00767THF_DHG_CYH_2THF_DH/CycHdrlase_CSPROSITE patterns
PR00085THFDHDRGNASETHF_DH/CycHdrlasePRINTS
G3DSA:3.10.410.10:FF:000001CATH-FunFam
G3DSA:3.40.50.10860:FF:000005CATH-FunFam
G3DSA:3.40.50.300:FF:000245CATH-FunFam
G3DSA:3.40.50.300:FF:005573CATH-FunFam
G3DSA:3.40.50.720:FF:000006CATH-FunFam
G3DSA:3.10.410.10CATH-Gene3D
G3DSA:3.40.50.10860CATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
G3DSA:3.40.50.720CATH-Gene3D
SSF51735NAD(P)-bd_dom_sfSUPERFAMILY
SSF52540P-loop_NTPaseSUPERFAMILY
SSF53223Aminoacid_DH-like_N_sfSUPERFAMILY
PTHR48099PANTHER
MF_01543FTHFSFormate_THF_ligaseHAMAP
MF_01576THF_DHG_CYHTHF_DH/CycHdrlaseHAMAP

Orthologs

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25633902 - Characterization and review of MTHFD1 deficiency: four new patients, cellular delineation and response to folic and folinic acid treatment.
Burda P et al. J Inherit Metab Dis 2015 Sep;38(5):863-72
GO_REF:0000033 - Annotation inferences using phylogenetic trees
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:25963819 - Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.
Lucena R et al. J Cell Biol 2015 May 11;209(3):387-402
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:499806 - Allelism of methionine-sensitive mutants of Schizosaccharomyces pombe to loci involved in adenine biosynthesis.
Strauss A Genet Res 1979 Jun;33(3):261-8
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
PMID:37859837 - In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions.
Mori A et al. R Soc Open Sci 2023 Oct;10(10):230404