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protein coding gene - hrs1 (SPBC2G2.12) - mitochondrial and cytoplasmic histidine-tRNA ligase Hrs1

Gene summary

Standard name
hrs1
Systematic ID
SPBC2G2.12
Product
mitochondrial and cytoplasmic histidine-tRNA ligase Hrs1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O43011
ORFeome ID
25/25H06
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome II: 3457165..3458912 forward strand

Annotation

PBO:0004521 - 6.1.1.21

Disease association

MONDO:0014711 - autosomal dominant Charcot-Marie-Tooth disease type 2W

References:

MONDO:0017312 - Perrault syndrome

References:

MONDO:0013972 - Perrault syndrome 2

References:

MONDO:0013788 - Usher syndrome type 3B

References:

GO biological process

GO:0002181 - cytoplasmic translation

References:

GO:0032543 - mitochondrial translation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005759 - mitochondrial matrix

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0004821 - histidine-tRNA ligase activity

References:

GO:0003723 - RNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF00458WHEP-TRSWHEP-TRS_domPFAM
PF13393tRNA-synt_HisHisRS-like_corePFAM
PF03129HGTP_anticodonAnticodon-bdPFAM
cd00773HisRS-like_coreHisRS-like_coreCDD
cd01200WHEPGMRS_RNACDD
cd00859HisRS_anticodonHisRS_anticodonCDD
PS00762WHEP_TRS_1WHEP-TRS_domPROSITE_PATTERNS
PS51185WHEP_TRS_2WHEP-TRS_domPROSITE_PROFILES
PS50862AA_TRNA_LIGASE_IIaa-tRNA-synth_IIPROSITE_PROFILES
SM00991WHEP_TRS_a_2_a_3_aWHEP-TRS_domSMART
G3DSA:3.30.930.10:FF:000021FUNFAM
G3DSA:3.40.50.800:FF:000015FUNFAM
SSF52954Class II aaRS ABD-relatedSUPERFAMILY
SSF47060S15/NS1 RNA-binding domainuS15_NS1_RNA-bd_sfSUPERFAMILY
SSF55681Class II aaRS and biotin synthetasesaa-tRNA-synth_II/BPL/LPLSUPERFAMILY
G3DSA:3.40.50.800Anticodon-bd_dom_sfGENE3D
G3DSA:3.30.930.10Bira Bifunctional Protein; Domain 2aa-tRNA-synth_II/BPL/LPLGENE3D
G3DSA:1.10.287.10GENE3D
PTHR11476HISTIDYL-TRNA SYNTHETASEPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte
TIGR00442hisSHis-tRNA-ligaseNCBIFAM

Orthologs

References / Literature

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:22425159 - Transient structure associated with the spindle pole body directs meiotic microtubule reorganization in S. pombe.
Funaya C et al. Curr Biol 2012 Apr 10;22(7):562-74
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PB_REF:0000003 - Disease Association Curation
PMID:24954111 - Chromosomes rein back the spindle pole body during horsetail movement in fission yeast meiosis.
Chikashige Y et al. Cell Struct Funct 2014;39(2):93-100
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000002 - Comments
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105