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protein coding gene - csi1 (SPBC2G2.14) - mitotic centromere-SPB clustering protein Csi1

Gene summary

Standard name
csi1
Systematic ID
SPBC2G2.14
Product
mitotic centromere-SPB clustering protein Csi1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O43013
ORFeome ID
25/25F12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3461338..3463865 forward strand

Annotation

GO biological process

GO:0072766 - centromere clustering at the mitotic interphase nuclear envelope

References:

GO:0000070 - mitotic sister chromatid segregation

References:

GO cellular component

GO:0000779 - condensed chromosome, centromeric region

References:

GO:0035974 - meiotic spindle pole body

References:

GO:0097431 - mitotic spindle pole

References:

GO:0044732 - mitotic spindle pole body

References:

GO:0005635 - nuclear envelope

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0030674 - protein-macromolecule adaptor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0006800 - decreased centromere clustering at nuclear periphery during mitotic interphase

References:

Genotypes:

FYPO:0003411 - decreased chromatin silencing at centromere inner repeat

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0000940 - decreased protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0005371 - increased linear minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0005917 - increased subtelomeric heterochromatin RNA level

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0001690 - normal growth on camptothecin

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0003840 - sensitive to carbendazim

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001779 - abnormal centromere clustering at nuclear periphery during vegetative growth

References:

Genotypes:

FYPO:0000030 - abnormal mitotic chromosome congression

References:

Genotypes:

FYPO:0000168 - abnormal mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0000443 - abnormal protein localization during vegetative growth

References:

Genotypes:

FYPO:0001269 - abolished protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0000941 - abolished protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0006825 - abolished protein localization to mitotic spindle pole body during mitosis

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0000227 - chromosome loss during mitotic chromosome segregation

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0006800 - decreased centromere clustering at nuclear periphery during mitotic interphase

References:

Genotypes:

FYPO:0000450 - decreased protein localization to centromere during vegetative growth

References:

Genotypes:

FYPO:0000940 - decreased protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0004648 - delayed onset of mitotic spindle assembly

References:

Genotypes:

FYPO:0003566 - delayed onset of mitotic spindle pole body separation

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0001846 - increased duration of mitotic anaphase A

References:

Genotypes:

FYPO:0005371 - increased linear minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0001861 - increased minichromosome loss upon segregation during vegetative growth

References:

Genotypes:

FYPO:0002969 - increased protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

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Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000276 - monopolar mitotic spindle

References:

Genotypes:

FYPO:0002360 - normal chromatin silencing at centromere

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0001690 - normal growth on camptothecin

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0005779 - normal protein localization to kinetochore during mitosis

References:

Genotypes:

FYPO:0002966 - normal protein localization to mitotic spindle

References:

Genotypes:

FYPO:0005602 - normal subtelomeric heterochromatin RNA level

References:

Genotypes:

FYPO:0000783 - protein mislocalized to cytoplasm during vegetative growth

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

References:

Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003840 - sensitive to carbendazim

References:

Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009114 - transient monopolar mitotic spindle

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0016885 - Schizosaccharomyces specific

Protein features

IDNameInterPro nameDB name
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

References / Literature

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:27334362 - Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions.
Tange Y et al. Genes Cells 2016 Aug;21(8):812-32
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26744419 - Control of heterochromatin localization and silencing by the nuclear membrane protein Lem2.
Barrales RR et al. Genes Dev 2016 Jan 15;30(2):133-48
PMID:23669133 - Csi1 illuminates the mechanism and function of Rabl configuration.
Hou H et al. Nucleus 2013;4(3):176-81
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
GO_REF:0000051 - S. pombe keyword mapping
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:35354597 - Quantitative analysis of nuclear pore complex organization in Schizosaccharomyces pombe .
Varberg JM et al. Life Sci Alliance 2022 Jul;5(7)
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:19805578 - Genome-wide mapping of myosin protein-RNA networks suggests the existence of specialized protein production sites.
Mata J FASEB J 2010 Feb;24(2):479-84
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:28678660 - The functionally elusive RabI chromosome configuration directly regulates nuclear membrane remodeling at mitotic onset.
Fernández-Álvarez A et al. Cell Cycle 2017 Aug 03;16(15):1392-1396
PMID:29021344 - A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.
Yukawa M et al. Mol Biol Cell 2017 Dec 01;28(25):3647-3659
PMID:26483559 - Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly.
Yang HJ et al. J Cell Biol 2015 Oct 26;211(2):295-308
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:25253718 - csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.
Costa J et al. Mol Biol Cell 2014 Dec 01;25(24):3900-8
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:29722648 - Distinct 'safe zones' at the nuclear envelope ensure robust replication of heterochromatic chromosome regions.
Ebrahimi H et al. Elife 2018 May 03;7
PMID:31483748 - Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast.
Zhurinsky J et al. Mol Biol Cell 2019 Oct 01;30(21):2695-2708
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:25057016 - Csi1p recruits alp7p/TACC to the spindle pole bodies for bipolar spindle formation.
Zheng F et al. Mol Biol Cell 2014 Sep 15;25(18):2750-60
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.
Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39786922 - The fission yeast SUMO-targeted ubiquitin ligase Slx8 functionally associates with clustered centromeres and the silent mating-type region at the nuclear periphery.
Chakraborty S et al. Biol Open 2024 Dec 15;13(12)
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:27889481 - Mitotic Nuclear Envelope Breakdown and Spindle Nucleation Are Controlled by Interphase Contacts between Centromeres and the Nuclear Envelope.
Fernández-Álvarez A et al. Dev Cell 2016 Dec 05;39(5):544-559