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protein coding gene - ctu1 (SPBC2G5.03) - cytosolic tRNA-uridine 2-sulfurtransferase subunit Ctu1

Gene summary

Standard name
ctu1
Systematic ID
SPBC2G5.03
Product
cytosolic tRNA-uridine 2-sulfurtransferase subunit Ctu1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94282
ORFeome ID
14/14H09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2581935..2583104 forward strand

Annotation

GO biological process

GO:0002143 - tRNA wobble position uridine thiolation

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GO cellular component

GO:0002144 - cytosolic tRNA wobble base thiouridylase complex

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GO molecular function

GO:0000049 - tRNA binding

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GO:0103016 - tRNA-uridine 2-sulfurtransferase activity

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Modification

MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0000961 - normal growth on sorbitol

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0000646 - swollen vegetative cell

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003218 - abolished tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridine biosynthesis

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Genotypes:

FYPO:0005177 - abolished tRNA wobble position uridine thiolation

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Genotypes:

FYPO:0004321 - altered DNA level during vegetative growth

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0001450 - resistance to cold

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009070 - resistance to itraconazole

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009039 - resistance to potassium chloride

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0007808 - resistance to valproic acid

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Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000106 - sensitive to hygromycin B

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0007938 - sensitive to tea tree oil

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

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Genotypes:

FYPO:0000646 - swollen vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01171ATP_bind_3TilS/TtcA_NPfam
PF16503zn-ribbon_14CTU1_CPfam
cd01713CTU1-likeCTU1-like_ATP-bdCDD
PS01263UPF0021UPF0021_CSPROSITE patterns
G3DSA:3.40.50.620:FF:000463CATH-FunFam
G3DSA:3.40.50.620Rossmann-like_a/b/a_foldCATH-Gene3D
SSF52402SUPERFAMILY
PTHR11807PANTHER
PIRSF004976ATPase_YdaOtRNA_thiolation_TtcA_Ctu1PIRSF
TIGR00269Ncs6/Tuc1/Ctu1NCBIFAM
MF_03053CTU1Ncs6/Tuc1/Ctu1HAMAP

Orthologs

References / Literature

PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:22768388 - Translational control of cell division by Elongator.
Bauer F et al. Cell Rep 2012 May 31;1(5):424-33
PMID:24223771 - New insights into the RNA-based mechanism of action of the anticancer drug 5'-fluorouracil in eukaryotic cells.
Mojardín L et al. PLoS One 2013;8(11):e78172
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:23874237 - Modification of tRNA(Lys) UUU by elongator is essential for efficient translation of stress mRNAs.
Fernández-Vázquez J et al. PLoS Genet 2013;9(7):e1003647
PMID:18391219 - The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity.
Dewez M et al. Proc Natl Acad Sci U S A 2008 Apr 08;105(14):5459-64
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:25651869 - Chemical genomics approach to identify genes associated with sensitivity to rapamycin in the fission yeast Schizosaccharomyces pombe.
Doi A et al. Genes Cells 2015 Apr;20(4):292-309
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:27255861 - Genetic evidence for involvement of membrane trafficking in the action of 5-fluorouracil.
Hu L et al. Fungal Genet Biol 2016 Aug;93:17-24