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protein coding gene - rpc25 (SPBC2G5.07c) - DNA-directed RNA polymerase III complex subunit Rpc25

Gene summary

Standard name
rpc25
Systematic ID
SPBC2G5.07c
Product
DNA-directed RNA polymerase III complex subunit Rpc25
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94285
ORFeome ID
32/32B01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2590901..2593385 reverse strand

Annotation

PBO:0000445 - 2.7.7.6

Disease association

MONDO:0009299 - 46 XX gonadal dysgenesis

References:

GO biological process

GO:0006384 - transcription initiation at RNA polymerase III promoter

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0005666 - RNA polymerase III complex

References:

GO molecular function

GO:0003677 - DNA binding

References:

GO:0003899 - DNA-directed RNA polymerase activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001971 - abnormal cell separation after cytokinesis resulting in chained cells

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03876SHS2_Rpb7-NRpb7-like_NPfam
PF08292RNA_pol_Rbc25RNA_pol_III_Rbc25Pfam
cd04330RNAP_III_Rpc25_NCDD
G3DSA:2.40.50.140:FF:000221CATH-FunFam
G3DSA:3.30.1490.120:FF:000001CATH-FunFam
G3DSA:2.40.50.140NA-bd_OB-foldCATH-Gene3D
G3DSA:3.30.1490.120RNA_pol_Rpb7-like_N_sfCATH-Gene3D
SSF50249NA-bd_OB-foldSUPERFAMILY
SSF88798RNA_pol_Rpb7-like_N_sfSUPERFAMILY
PTHR12709Rpb7-likePANTHER
TIGR00448rpoERNAP_E/RPC8NCBIFAM

Orthologs

References / Literature

PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
GO_REF:0000002 - Comments
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:21714024 - Crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe RNA polymerase III.
Ehara H et al. Protein Sci 2011 Sep;20(9):1558-65
PMID:25392932 - RNA processing factors Swd2.2 and Sen1 antagonize RNA Pol III-dependent transcription and the localization of condensin at Pol III genes.
Legros P et al. PLoS Genet 2014 Nov;10(11):e1004794
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:19910488 - Centromeric localization of dispersed Pol III genes in fission yeast.
Iwasaki O et al. Mol Biol Cell 2010 Jan 15;21(2):254-65
PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:31294478 - Senataxin homologue Sen1 is required for efficient termination of RNA polymerase III transcription.
Rivosecchi J et al. EMBO J 2019 Aug 15;38(16):e101955
PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.
Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24945319 - CPF-associated phosphatase activity opposes condensin-mediated chromosome condensation.
Vanoosthuyse V et al. PLoS Genet 2014 Jun;10(6):e1004415
PB_REF:0000003 - Disease Association Curation
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.
Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:15612920 - Rpc25, a conserved RNA polymerase III subunit, is critical for transcription initiation.
Zaros C et al. Mol Microbiol 2005 Jan;55(1):104-14