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protein coding gene - eri1 (SPBC30B4.08) - double-strand siRNA ribonuclease Eri1

Gene summary

Standard name
eri1
Systematic ID
SPBC30B4.08
Product
double-strand siRNA ribonuclease Eri1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q08I43
ORFeome ID
18/18A07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1320720..1323215 forward strand

Annotation

Disease association

MONDO:0958005 - Hoxha-Aliu syndrome

References:

MONDO:0958006 - spondyloepimetaphyseal dysplasia, Guo-Campeau type

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GO biological process

GO:0000467 - exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

References:

GO:0060906 - negative regulation of regulatory ncRNA-mediated heterochromatin formation

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0000175 - 3'-5'-RNA exonuclease activity

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GO:0003725 - double-stranded RNA binding

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GO:0032296 - double-stranded RNA-specific ribonuclease activity

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Protein features

PBO:0111804 - SAP domain

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0006298 - abnormal 5.8S rRNA 3'-end processing

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Genotypes:

FYPO:0002133 - abolished protein-RNA interaction

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Genotypes:

FYPO:0006301 - abolished ribonuclease activity

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0003094 - decreased centromeric outer repeat transcript level

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Genotypes:

FYPO:0003822 - decreased ribonuclease activity

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Genotypes:

FYPO:0006297 - increased 5.8S rRNA length

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Genotypes:

FYPO:0002836 - increased centromeric outer repeat transcript-derived siRNA level

References:

Genotypes:

FYPO:0006299 - increased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0004376 - increased chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0000875 - increased histone H3-K9 dimethylation at centromere inner repeat during vegetative growth

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Genotypes:

FYPO:0000887 - increased histone H3-K9 dimethylation at centromere outer repeat during vegetative growth

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Genotypes:

FYPO:0003009 - increased protein localization to centromere outer repeat

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Genotypes:

FYPO:0006300 - increased protein localization to heterochromatin at centromere inner repeat

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0004295 - multiseptate cell

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002546 - sensitive to trichostatin A

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF00929RNase_TRibonucl_HPfam
cd06133ERI-1_3'hExo_likeERI-1_3'hExo-likeCDD
PS50800SAPSAP_domPROSITE profiles
SM00479EXOIIIRibonucl_HSMART
G3DSA:3.30.420.10RNaseH_sfCATH-Gene3D
SSF53098RNaseH-like_sfSUPERFAMILY
PTHR230443-5_ExoribonucleasePANTHER
CoilCOILS

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18438419 - The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi.
Gabel HW et al. Nat Struct Mol Biol 2008 May;15(5):531-3
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:16797182 - Conserved ribonuclease, Eri1, negatively regulates heterochromatin assembly in fission yeast.
Iida T et al. Curr Biol 2006 Jul 25;16(14):1459-64
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704