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protein coding gene - pep12 (SPBC31E1.04) - endosomal membrane t-SNARE Pep12

Gene summary

Standard name
pep12
Systematic ID
SPBC31E1.04
Product
endosomal membrane t-SNARE Pep12
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94651
ORFeome ID
17/17H12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 245510..247128 forward strand

Annotation

GO biological process

GO:0045022 - early endosome to late endosome transport

References:

GO:0006896 - Golgi to vacuole transport

References:

GO cellular component

GO:0000329 - fungal-type vacuole membrane

References:

GO:0005770 - late endosome

References:

GO:0031902 - late endosome membrane

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GO molecular function

GO:0005484 - SNAP receptor activity

References:

Modification

MOD:00818 - glycosylphosphatidylinositolated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein features

PBO:0111768 - SNARE

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0004250 - abolished protein localization to vacuolar membrane

References:

Genotypes:

FYPO:0000544 - abolished protein modification during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000539 - increased protein secretion during vegetative growth

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000098 - sensitive to calcium

References:

Genotypes:

FYPO:0000281 - small vacuoles present in increased numbers during vegetative growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF05739SNARET_SNARE_domPfam
PF14523Syntaxin_2Syntaxin_NPfam
cd15840SNARE_QaCDD
PS50192T_SNARET_SNARE_domPROSITE profiles
SM00397t_SNARET_SNARE_domSMART
SM00503SynNSyntaxin_NSMART
G3DSA:1.20.5.110:FF:000059CATH-FunFam
G3DSA:1.20.5.110CATH-Gene3D
G3DSA:1.20.58.70CATH-Gene3D
SSF47661SNARESUPERFAMILY
PTHR19957SyntaxinPANTHER
Transmembrane alpha helixDeepTMHMM
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PB_REF:0000007 - Prediction of GPI-anchored proteins with pointer neural networks
PMID:14575697 - Identification of a SNARE protein required for vacuolar protein transport in Schizosaccharomyces pombe.
Takegawa K et al. Biochem Biophys Res Commun 2003 Nov 07;311(1):77-82
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21757403 - Schizosaccharomyces pombe Pep12p is required for vacuolar protein transport and vacuolar homotypic fusion.
Hosomi A et al. J Biosci Bioeng 2011 Oct;112(4):309-14
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25378562 - Vsl1p cooperates with Fsv1p for vacuolar protein transport and homotypic fusion in Schizosaccharomyces pombe.
Hosomi A et al. Microbiology (Reading) 2015 Jan;161(Pt 1):89-98
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:31341193 - Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome.
Yanguas F et al. Sci Rep 2019 Jul 24;9(1):10747
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:33788833 - Analysis of the SNARE Stx8 recycling reveals that the retromer-sorting motif has undergone evolutionary divergence.
Yanguas F et al. PLoS Genet 2021 Mar;17(3):e1009463
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405