protein coding gene - cwf4 (SPBC31F10.11c) - Prp19 complex subunit Cwf4

Gene summary

Standard name

cwf4

Systematic ID

SPBC31F10.11c

Product

Prp19 complex subunit Cwf4

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

syf3

UniProt ID

P87312

ORFeome ID

46/46C09

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 3770856..3773149 reverse strand

Annotation

Protein features

Orthologs

• CRNKL1 (HGNC:15762) Homo sapiens
• cwf4 (SJAG_00911) Schizosaccharomyces japonicus
• CLF1 (YLR117C) Saccharomyces cerevisiae

References / Literature

PMID:26292707 - Structure of a yeast spliceosome at 3.6-angstrom resolution.

Yan C et al. Science 2015 Sep 11;349(6253):1182-91

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .

Budden AM et al. Elife 2023 Aug 24;12

PMID:41201383 - Combinatorial Analyses of Pre-mRNA Splicing-Defective Mutants Reveal Differential Quantitative Requirements for Shelterin in Distinct Telomere Functions.

Takeuchi M et al. Genes Cells 2025 Nov;30(6):e70064

PMID:34209806 - Identification of Nrl1 Domains Responsible for Interactions with RNA-Processing Factors and Regulation of Nrl1 Function by Phosphorylation.

Mikolaskova B et al. Int J Mol Sci 2021 Jun 29;22(13)

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.

Bayne EH et al. Genome Biol 2014;15(10):481

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:25245948 - Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length.

Wang J et al. Nucleic Acids Res 2014 Oct;42(18):11419-32

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:24442611 - Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.

Chen W et al. RNA 2014 Mar;20(3):308-20

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:10409726 - Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing.

McDonald WH et al. Mol Cell Biol 1999 Aug;19(8):5352-62

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:15755920 - Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex.

Carnahan RH et al. Eukaryot Cell 2005 Mar;4(3):577-87

PMID:24081329 - Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.

Grenier St-Sauveur V et al. Mol Cell Biol 2013 Dec;33(23):4718-31

PMID:24014766 - Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.

Livesay SB et al. Eukaryot Cell 2013 Nov;12(11):1472-89

PMID:26682798 - The spliceosome-associated protein Nrl1 suppresses homologous recombination-dependent R-loop formation in fission yeast.

Aronica L et al. Nucleic Acids Res 2016 Feb 29;44(4):1703-17

PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.

Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101

PMID:11884590 - Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.

Ohi MD et al. Mol Cell Biol 2002 Apr;22(7):2011-24

PMID:31219728 - Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe .

Cipakova I et al. Cell Cycle 2019 Jul;18(14):1532-1536

PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Das J et al. Sci Signal 2013 May 21;6(276):ra38

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.

Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684

PMID:12088152 - Characterization of interactions among the Cef1p-Prp19p-associated splicing complex.

Ohi MD et al. RNA 2002 Jun;8(6):798-815

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.

Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .

Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Ren L et al. PLoS One 2011 Feb 28;6(2):e16719

PMID:24874881 - The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.

Kallgren SP et al. PLoS Genet 2014;10(5):e1004334

PMID:39833470 - Structures of aberrant spliceosome intermediates on their way to disassembly.

Soni K et al. Nat Struct Mol Biol 2025 Jan 20;

GO_REF:0000033 - Annotation inferences using phylogenetic trees