protein coding gene - gtb1 (SPBC32F12.04) - gamma-tubulin Gtb1

Gene summary

Standard name

gtb1

Systematic ID

SPBC32F12.04

Product

gamma-tubulin Gtb1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

tug1

UniProt ID

P25295

ORFeome ID

40/40F08

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 2791351..2794184 forward strand

Annotation

Protein features

Orthologs

• TUBG1 (HGNC:12417) Homo sapiens
• TUBG2 (HGNC:12419) Homo sapiens
• gtb1 (SJAG_01215) Schizosaccharomyces japonicus
• TUB4 (YLR212C) Saccharomyces cerevisiae

References / Literature

PMID:11082048 - Identification of an alpha-tubulin mutant of fission yeast from gamma-tubulin-interacting protein screening: genetic evidence for alpha-/gamma-tubulin interaction.

Takeoka A et al. J Cell Sci 2000 Dec;113 Pt 24:4557-62

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:11080156 - The fission yeast gamma-tubulin complex is required in G(1) phase and is a component of the spindle assembly checkpoint.

Vardy L et al. EMBO J 2000 Nov 15;19(22):6098-111

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:8089179 - Human gamma-tubulin functions in fission yeast.

Horio T et al. J Cell Biol 1994 Sep;126(6):1465-73

PMID:12402243 - Molecular and structural characterization of the spindle pole bodies in the fission yeast Schizosaccharomyces japonicus var japonicus.

Horio T et al. Yeast 2002 Nov;19(15):1335-50

PMID:16611237 - Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexes.

Masuda H et al. Genes Cells 2006 Apr;11(4):319-36

PMID:16855399 - An extended anaphase signaling pathway for Mad2p includes microtubule organizing center proteins and multiple motor-dependent transitions.

Mayer C et al. Cell Cycle 2006 Jul;5(13):1456-63

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:25348260 - Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells.

Olmsted ZT et al. Nat Commun 2014 Oct 28;5:5339

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23388459 - Kinesin-14 Pkl1 targets γ-tubulin for release from the γ-tubulin ring complex (γ-TuRC) ‬‬‬‬‬‬‬.

Olmsted ZT et al. Cell Cycle 2013 Mar 01;12(5):842-8

PMID:19942852 - Fission yeast Pcp1 links polo kinase-mediated mitotic entry to gamma-tubulin-dependent spindle formation.

Fong CS et al. EMBO J 2010 Jan 06;29(1):120-30

PMID:10602257 - Lethal level overexpression of gamma-tubulin in fission yeast causes mitotic arrest.

Horio T et al. Cell Motil Cytoskeleton 1999 Dec;44(4):284-95

PMID:15004232 - Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function.

Venkatram S et al. Mol Biol Cell 2004 May;15(5):2287-301

PMID:19001497 - Two distinct regions of Mto1 are required for normal microtubule nucleation and efficient association with the gamma-tubulin complex in vivo.

Samejima I et al. J Cell Sci 2008 Dec 01;121(Pt 23):3971-80

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:15280226 - Functional dissection of the gamma-tubulin complex by suppressor analysis of gtb1 and alp4 mutations in Schizosaccharomyces pombe.

Tange Y et al. Genetics 2004 Jul;167(3):1095-107

PMID:17277378 - Novel mad2 alleles isolated in a Schizosaccharomyces pombe gamma-tubulin mutant are defective in metaphase arrest activity, but remain functional for chromosome stability in unperturbed mitosis.

Tange Y et al. Genetics 2007 Apr;175(4):1571-84

PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.

Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643

PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119

PMID:18418055 - Protein complexes at the microtubule organizing center regulate bipolar spindle assembly.

Rodriguez AS et al. Cell Cycle 2008 May 01;7(9):1246-53

PMID:28619713 - Molecular model of fission yeast centrosome assembly determined by superresolution imaging.

Bestul AJ et al. J Cell Biol 2017 Aug 07;216(8):2409-2424

PMID:22737087 - The CCR4-NOT complex is implicated in the viability of aneuploid yeasts.

Tange Y et al. PLoS Genet 2012;8(6):e1002776

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:26243668 - Mto2 multisite phosphorylation inactivates non-spindle microtubule nucleation complexes during mitosis.

Borek WE et al. Nat Commun 2015 Aug 05;6:7929

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:24704079 - Activation of the γ-tubulin complex by the Mto1/2 complex.

Lynch EM et al. Curr Biol 2014 Apr 14;24(8):896-903

GO_REF:0000002 - Comments

PMID:24006493 - Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3(Alp6).

Dhani DK et al. Mol Biol Cell 2013 Nov;24(21):3337-49

PMID:9742398 - Conjugation in S. pombe: identification of a microtubule-organising centre, a requirement for microtubules and a role for Mad2.

Petersen J et al. Curr Biol 1998 Aug 27;8(17):963-6

PMID:10749926 - A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p.

Paluh JL et al. Mol Biol Cell 2000 Apr;11(4):1225-39

PMID:12134075 - A fourth component of the fission yeast gamma-tubulin complex, Alp16, is required for cytoplasmic microtubule integrity and becomes indispensable when gamma-tubulin function is compromised.

Fujita A et al. Mol Biol Cell 2002 Jul;13(7):2360-73

PMID:12791993 - Equatorial retention of the contractile actin ring by microtubules during cytokinesis.

Pardo M et al. Science 2003 Jun 06;300(5625):1569-74

PMID:17021256 - Noncore components of the fission yeast gamma-tubulin complex.

Anders A et al. Mol Biol Cell 2006 Dec;17(12):5075-93

PMID:1770000 - The fission yeast gamma-tubulin is essential for mitosis and is localized at microtubule organizing centers.

Horio T et al. J Cell Sci 1991 Aug;99 ( Pt 4):693-700

PMID:23885124 - Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly.

Masuda H et al. Mol Biol Cell 2013 Sep;24(18):2894-906

PMID:24055157 - CK1 is required for a mitotic checkpoint that delays cytokinesis.

Johnson AE et al. Curr Biol 2013 Oct 07;23(19):1920-6

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:37783794 - An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.

Ader NR et al. Nat Cell Biol 2023 Oct;25(10):1465-1477

PMID:20850323 - A catalytic role for Mod5 in the formation of the Tea1 cell polarity landmark.

Bicho CC et al. Curr Biol 2010 Oct 12;20(19):1752-7

PMID:8834801 - Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body.

Masuda H et al. J Cell Sci 1996 Jan;109 ( Pt 1):165-77