protein coding gene - rum1 (SPBC32F12.09) - CDK inhibitor Rum1

Gene summary

Standard name

rum1

Systematic ID

SPBC32F12.09

Product

CDK inhibitor Rum1

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

P40380

ORFeome ID

31/31G12

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 2799677..2801917 forward strand

Annotation

Protein features

Orthologs

• rum1 (SJAG_01210) Schizosaccharomyces japonicus

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:9614176 - Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast.

Stern B et al. Mol Biol Cell 1998 Jun;9(6):1309-21

PMID:12135491 - Rum1, an inhibitor of cyclin-dependent kinase in fission yeast, is negatively regulated by mitogen-activated protein kinase-mediated phosphorylation at Ser and Thr residues.

Matsuoka K et al. Eur J Biochem 2002 Jul;269(14):3511-21

PMID:10725227 - A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls.

Grallert B et al. J Cell Sci 2000 Apr;113 ( Pt 8):1447-58

PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.

Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72

PMID:22144913 - Widespread cotranslational formation of protein complexes.

Duncan CD et al. PLoS Genet 2011 Dec;7(12):e1002398

PMID:9013342 - The size control of fission yeast revisited.

Sveiczer A et al. J Cell Sci 1996 Dec;109 ( Pt 12):2947-57

PMID:23376070 - Fission yeast LAMMER kinase Lkh1 regulates the cell cycle by phosphorylating the CDK-inhibitor Rum1.

Yu EY et al. Biochem Biophys Res Commun 2013 Mar 01;432(1):80-5

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:10388806 - Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes.

Snaith HA et al. Genetics 1999 Jul;152(3):839-51

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:9398669 - A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes.

Yamaguchi S et al. Mol Biol Cell 1997 Dec;8(12):2475-86

PMID:17046992 - Fission yeast Tor2 promotes cell growth and represses cell differentiation.

Alvarez B et al. J Cell Sci 2006 Nov 01;119(Pt 21):4475-85

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:9436991 - Negative regulation of Cdc18 DNA replication protein by Cdc2.

Lopez-Girona A et al. Mol Biol Cell 1998 Jan;9(1):63-73

PMID:9571240 - Fission yeast Ste9, a homolog of Hct1/Cdh1 and Fizzy-related, is a novel negative regulator of cell cycle progression during G1-phase.

Kitamura K et al. Mol Biol Cell 1998 May;9(5):1065-80

PMID:10921876 - APC(ste9/srw1) promotes degradation of mitotic cyclins in G(1) and is inhibited by cdc2 phosphorylation.

Blanco MA et al. EMBO J 2000 Aug 01;19(15):3945-55

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:11163211 - The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases.

Yamano H et al. Mol Cell 2000 Dec;6(6):1377-87

PMID:36779416 - The ecl family gene ecl3+ is induced by phosphate starvation and contributes to sexual differentiation in fission yeast.

Ohtsuka H et al. J Cell Sci 2023 Mar 15;136(6)

PMID:9653157 - sud1(+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast.

Jallepalli PV et al. Proc Natl Acad Sci U S A 1998 Jul 07;95(14):8159-64

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:9736616 - Apc10 and Ste9/Srw1, two regulators of the APC-cyclosome, as well as the CDK inhibitor Rum1 are required for G1 cell-cycle arrest in fission yeast.

Kominami K et al. EMBO J 1998 Sep 15;17(18):5388-99

PMID:15194812 - A novel intermediate in initiation complex assembly for fission yeast DNA replication.

Yamada Y et al. Mol Biol Cell 2004 Aug;15(8):3740-50

PMID:10679013 - The puc1 cyclin regulates the G1 phase of the fission yeast cell cycle in response to cell size.

Martín-Castellanos C et al. Mol Biol Cell 2000 Feb;11(2):543-54

PMID:12167173 - Combinatorial diversity of fission yeast SCF ubiquitin ligases by homo- and heterooligomeric assemblies of the F-box proteins Pop1p and Pop2p.

Seibert V et al. BMC Biochem 2002 Aug 07;3:22

PMID:8598285 - Rum1 and Cdc18 link inhibition of cyclin-dependent kinase to the initiation of DNA replication in Schizosaccharomyces pombe.

Jallepalli PV et al. Genes Dev 1996 Mar 01;10(5):541-52

PMID:11937031 - CDK phosphorylation of Drc1 regulates DNA replication in fission yeast.

Noguchi E et al. Curr Biol 2002 Apr 02;12(7):599-605

PMID:8521500 - p25rum1 orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase.

Correa-Bordes J et al. Cell 1995 Dec 15;83(6):1001-9

PMID:15498101 - Germinating fission yeast spores delay in G1 in response to UV irradiation.

Nilssen EA et al. BMC Cell Biol 2004 Oct 21;5(1):40

PMID:32084401 - The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis.

Basu S et al. Curr Biol 2020 Mar 09;30(5):883-892.e4

PMID:23843946 - The role of the RACK1 ortholog Cpc2p in modulating pheromone-induced cell cycle arrest in fission yeast.

Mos M et al. PLoS One 2013;8(7):e65927

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:9472077 - The Ste16 WD-repeat protein regulates cell-cycle progression under starvation through the Rum1 protein in Schizosaccharomyces pombe.

Maekawa H et al. Curr Genet 1998 Jan;33(1):29-37

PMID:7883794 - Regulation of the cell cycle timing of Start in fission yeast by the rum1+ gene.

Moreno S et al. J Cell Sci Suppl 1994;18:63-8

PMID:9739083 - Cdc18p can block mitosis by two independent mechanisms.

Greenwood E et al. J Cell Sci 1998 Oct;111 ( Pt 20):3101-8

PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80

PMID:9303310 - p25rum1 promotes proteolysis of the mitotic B-cyclin p56cdc13 during G1 of the fission yeast cell cycle.

Correa-Bordes J et al. EMBO J 1997 Aug 01;16(15):4657-64

PMID:19366728 - Genetic control of cellular quiescence in S. pombe.

Sajiki K et al. J Cell Sci 2009 May 01;122(Pt 9):1418-29

PMID:15195092 - Periodic gene expression program of the fission yeast cell cycle.

Rustici G et al. Nat Genet 2004 Aug;36(8):809-17

PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.

Chen JS et al. Yeast 2016 Sep;33(9):507-17

PMID:9430640 - Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor.

Benito J et al. EMBO J 1998 Jan 15;17(2):482-97

PMID:10209119 - F-box/WD-repeat proteins pop1p and Sud1p/Pop2p form complexes that bind and direct the proteolysis of cdc18p.

Wolf DA et al. Curr Biol 1999 Apr 08;9(7):373-6

PMID:11329175 - Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe.

Tasto JJ et al. Yeast 2001 May;18(7):657-62

PMID:19033384 - The G1-S checkpoint in fission yeast is not a general DNA damage checkpoint.

Krohn M et al. J Cell Sci 2008 Dec 15;121(Pt 24):4047-54

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:9990507 - Two F-box/WD-repeat proteins Pop1 and Pop2 form hetero- and homo-complexes together with cullin-1 in the fission yeast SCF (Skp1-Cullin-1-F-box) ubiquitin ligase.

Kominami K et al. Genes Cells 1998 Nov;3(11):721-35

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:11781565 - Feedback regulation of the MBF transcription factor by cyclin Cig2.

Ayté J et al. Nat Cell Biol 2001 Dec;3(12):1043-50

PMID:8121488 - Regulation of progression through the G1 phase of the cell cycle by the rum1+ gene.

Moreno S et al. Nature 1994 Jan 20;367(6460):236-42

PMID:10036243 - Regulation of the start of DNA replication in Schizosaccharomyces pombe.

Carlson CR et al. J Cell Sci 1999 Mar;112 ( Pt 6):939-46

PMID:18235227 - Fission yeast TOR complex 2 activates the AGC-family Gad8 kinase essential for stress resistance and cell cycle control.

Ikeda K et al. Cell Cycle 2008 Feb 01;7(3):358-64

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:20634885 - TOR and PKA pathways synergize at the level of the Ste11 transcription factor to prevent mating and meiosis in fission yeast.

Valbuena N et al. PLoS One 2010 Jul 09;5(7):e11514

PMID:7593289 - Interaction of cdc2 and rum1 regulates Start and S-phase in fission yeast.

Labib K et al. J Cell Sci 1995 Oct;108 ( Pt 10):3285-94

PMID:9552380 - Regulation of G1 progression in fission yeast by the rum1+ gene product.

Martín-Castellanos C et al. Prog Cell Cycle Res 1996;2:29-35

PMID:32361273 - Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe.

Stonyte V et al. iScience 2020 May 22;23(5):101063

PMID:9472012 - The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast.

Sánchez-Díaz A et al. J Cell Sci 1998 Mar;111 ( Pt 6):843-51

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:30680437 - Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication.

García-Blanco N et al. Curr Genet 2019 Jun;65(3):685-690

PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.

Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643

PMID:30154212 - Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription.

Rubio A et al. J Cell Sci 2018 Sep 20;131(18)

PMID:9203581 - Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18.

Kominami K et al. Genes Dev 1997 Jun 15;11(12):1548-60

PMID:14970237 - Requirement of the SCFPop1/Pop2 Ubiquitin Ligase for Degradation of the Fission Yeast S Phase Cyclin Cig2.

Yamano H et al. J Biol Chem 2004 Apr 30;279(18):18974-80