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protein coding gene - git5 (SPBC32H8.07) - heterotrimeric G protein beta (WD repeat) subunit Git5

Gene summary

Standard name
git5
Systematic ID
SPBC32H8.07
Product
heterotrimeric G protein beta (WD repeat) subunit Git5
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
gpb1
UniProt ID
Q10282
ORFeome ID
41/41G09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1463849..1465745 forward strand

Annotation

Disease association

MONDO:0014074 - Charcot-Marie-Tooth disease dominant intermediate F

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MONDO:0014872 - congenital stationary night blindness 1H

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MONDO:0007781 - essential hypertension, genetic

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MONDO:0014953 - gnb5-related intellectual disability-cardiac arrhythmia syndrome

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MONDO:0014855 - intellectual disability, autosomal dominant 42

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MONDO:0014957 - language delay and attention deficit-hyperactivity disorder/cognitive impairment with or without cardiac arrhythmia

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MONDO:0013108 - leukemia, acute lymphocytic, susceptibility to, 1

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MONDO:0018881 - myelodysplastic syndrome

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MONDO:0859185 - neurodevelopmental disorder with hypotonia and dysmorphic facies

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MONDO:0859173 - sick sinus syndrome 4

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GO biological process

GO:0010619 - adenylate cyclase-activating glucose-activated G protein-coupled receptor signaling pathway

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GO:0010515 - negative regulation of induction of conjugation with cellular fusion

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GO:0000122 - negative regulation of transcription by RNA polymerase II

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GO cellular component

GO:0005829 - cytosol

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GO:0031680 - G-protein beta/gamma-subunit complex

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GO:0005834 - heterotrimeric G-protein complex

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GO:0005634 - nucleus

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GO molecular function

GO:0005515 - protein binding

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GO:0030159 - signaling receptor complex adaptor activity

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Multi-locus phenotype

FYPO:0006993 - decreased chromatin silencing at centromere otr1R

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Genotypes:

FYPO:0003120 - decreased transcription during glucose starvation

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Genotypes:

FYPO:0001043 - increased mating efficiency

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Genotypes:

FYPO:0001865 - normal negative regulation of transcription by glucose

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000280 - sterile

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Genotypes:

Protein features

PBO:0111751 - WD repeat protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000240 - abnormal filament morphology

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Genotypes:

FYPO:0000044 - abnormal negative regulation of transcription by glucose

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Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0001665 - decreased cellular cAMP level during cellular response to glucose stimulus

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Genotypes:

FYPO:0005288 - decreased negative regulation of transcription by glucose

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Genotypes:

FYPO:0000552 - decreased pseudohyphal growth

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Genotypes:

FYPO:0003770 - decreased transcriptional response to pheromone

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0009095 - increased cell population growth on fructose carbon source

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

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Genotypes:

FYPO:0005279 - increased level of glucose-repressed gene mRNA during vegetative growth

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Genotypes:

FYPO:0001043 - increased mating efficiency

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0000780 - increased transcription during vegetative growth

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0001666 - normal cAMP level

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Genotypes:

FYPO:0007035 - normal growth on 5-fluorouracil

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Genotypes:

FYPO:0002343 - normal growth on terbinafine

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Genotypes:

FYPO:0001147 - normal mating efficiency

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Genotypes:

FYPO:0001865 - normal negative regulation of transcription by glucose

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Genotypes:

FYPO:0004600 - normal transcriptional response to pheromone

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000102 - sensitive to cisplatin

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Genotypes:

FYPO:0002640 - sensitive to clotrimazole

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0003559 - sensitive to doxorubicin

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

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Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

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Genotypes:

FYPO:0003612 - viable spore population

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0003635 - S. cerevisiae YOR212W is not functional ortholog

Protein features

IDNameInterPro nameDB name
PF25391WD40_GbetaG-protein_beta_1-5PFAM
cd00200WD40CDD
PS00678WD_REPEATS_1WD40_repeat_CSPROSITE_PATTERNS
PS50082WD_REPEATS_2WD40_rptPROSITE_PROFILES
PS50294WD_REPEATS_REGIONPROSITE_PROFILES
SM00320WD40_4WD40_rptSMART
PR00320GPROTEINBRPTWD40_PAC1PRINTS
PR00319GPROTEINBWD40_G-protein_beta-likePRINTS
G3DSA:2.130.10.10:FF:000580FUNFAM
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
PTHR19850GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETAG-protein_beta_1-5PANTHER
PIRSF002394GNBP_BPIRSF

Orthologs

References / Literature

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.
Takeda K et al. PLoS One 2011;6(7):e22021
PMID:36358992 - Multi-Layered Regulations on the Chromatin Architectures: Establishing the Tight and Specific Responses of Fission Yeast fbp1 Gene Transcription.
Asada R et al. Biomolecules 2022 Nov 05;12(11)
PMID:35171902 - The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly.
Bao K et al. PLoS Genet 2022 Feb;18(2):e1010049
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:7498728 - sck1, a high copy number suppressor of defects in the cAMP-dependent protein kinase pathway in fission yeast, encodes a protein homologous to the Saccharomyces cerevisiae SCH9 kinase.
Jin M et al. Genetics 1995 Jun;140(2):457-67
PMID:8227198 - Six git genes encode a glucose-induced adenylate cyclase activation pathway in the fission yeast Schizosaccharomyces pombe.
Byrne SM et al. J Cell Sci 1993 Aug;105 ( Pt 4)(0 4):1095-100
PMID:1849107 - Glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene occurs by a cAMP signaling pathway.
Hoffman CS et al. Genes Dev 1991 Apr;5(4):561-71
PMID:19542312 - Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe.
Dodgson J et al. Eukaryot Cell 2009 Aug;8(8):1298-306
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:8804400 - The G protein beta subunit Gpb1 of Schizosaccharomyces pombe is a negative regulator of sexual development.
Kim DU et al. Mol Gen Genet 1996 Aug 27;252(1-2):20-32
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:23365689 - Cellular robustness conferred by genetic crosstalk underlies resistance against chemotherapeutic drug doxorubicin in fission yeast.
Tay Z et al. PLoS One 2013;8(1):e55041
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:20531409 - Global coordination of transcriptional control and mRNA decay during cellular differentiation.
Amorim MJ et al. Mol Syst Biol 2010 Jun 08;6:380
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33064911 - Genes affecting the extension of chronological lifespan in Schizosaccharomyces pombe (fission yeast).
Ohtsuka H et al. Mol Microbiol 2021 Apr;115(4):623-642
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:35286199 - Mitotic spindle formation in the absence of Polo kinase.
Kim J et al. Proc Natl Acad Sci U S A 2022 Mar 22;119(12):e2114429119
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:11014802 - Glucose monitoring in fission yeast via the Gpa2 galpha, the git5 Gbeta and the git3 putative glucose receptor.
Welton RM et al. Genetics 2000 Oct;156(2):513-21
PMID:16884933 - Identification of Gnr1p, a negative regulator of G alpha signalling in Schizosaccharomyces pombe, and its complementation by human G beta subunits.
Goddard A et al. Fungal Genet Biol 2006 Dec;43(12):840-51
PMID:20139237 - Activated alleles of the Schizosaccharomyces pombe gpa2+ Galpha gene identify residues involved in GDP-GTP exchange.
Ivey FD et al. Eukaryot Cell 2010 Apr;9(4):626-33
PMID:10747045 - The fission yeast git5 gene encodes a Gbeta subunit required for glucose-triggered adenylate cyclase activation.
Landry S et al. Genetics 2000 Apr;154(4):1463-71
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:18430926 - Schizosaccharomyces pombe Hsp90/Git10 is required for glucose/cAMP signaling.
Alaamery MA et al. Genetics 2008 Apr;178(4):1927-36
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:11238401 - The git5 Gbeta and git11 Ggamma form an atypical Gbetagamma dimer acting in the fission yeast glucose/cAMP pathway.
Landry S et al. Genetics 2001 Mar;157(3):1159-68
PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.
Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)
PMID:8001792 - Glucose repression of fbp1 transcription of Schizosaccharomyces pombe is partially regulated by adenylate cyclase activation by a G protein alpha subunit encoded by gpa2 (git8).
Nocero M et al. Genetics 1994 Sep;138(1):39-45
PMID:26791325 - Predicting chemotherapeutic drug combinations through gene network profiling.
Nguyen TT et al. Sci Rep 2016 Jan 21;6:18658
PMID:8832414 - The Schizosaccharomyces pombe pyp1 protein tyrosine phosphatase negatively regulates nutrient monitoring pathways.
Santo PD et al. J Cell Sci 1996 Jul;109 ( Pt 7)(0 7):1919-1925
PMID:25669599 - Fitness profiling links topoisomerase II regulation of centromeric integrity to doxorubicin resistance in fission yeast.
Nguyen TT et al. Sci Rep 2015 Feb 11;5:8400
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:2157626 - Isolation and characterization of mutants constitutive for expression of the fbp1 gene of Schizosaccharomyces pombe.
Hoffman CS et al. Genetics 1990 Apr;124(4):807-16
PMID:22624651 - A systematic screen reveals new elements acting at the G2/M cell cycle control.
Navarro FJ et al. Genome Biol 2012 May 24;13(5):R36
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:24928510 - Glucose activates TORC2-Gad8 protein via positive regulation of the cAMP/cAMP-dependent protein kinase A (PKA) pathway and negative regulation of the Pmk1 protein-mitogen-activated protein kinase pathway.
Cohen A et al. J Biol Chem 2014 Aug 01;289(31):21727-37
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838