protein coding gene - wdr8 (SPBC32H8.09) - mitosis-specific spindle pole body WD repeat protein Wdr8

Gene summary

Standard name: wdr8
Systematic ID: SPBC32H8.09
Product: mitosis-specific spindle pole body WD repeat protein Wdr8
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: pi015, SPACTOKYO_453.21c
UniProt ID: Q96WW0
ORFeome ID: 50/50H03
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome II: 1468510..1470473 forward strand

Annotation

GO biological process

GO:1990810 - microtubule anchoring at mitotic spindle pole body

References:

PMID:25987607

GO:0000070 - mitotic sister chromatid segregation

References:

PMID:25987607

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0072686 - mitotic spindle

References:

PMID:25987607

GO:0044732 - mitotic spindle pole body

References:

GO:1990811 - MWP complex

References:

PMID:25987607

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

PMID:25987607

Multi-locus phenotype

FYPO:0003787 - long mitotic spindle microtubules protruding beyond spindle pole body

References:

PMID:25987607

Genotypes:

msd1delta wdr8delta

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111751 - WD repeat protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PMID:39367033

PomGeneEx:0000011 - RNA level increased

References:

PMID:12161753

Quantitative gene expression

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0004755 - abnormal protein localization to microtubule minus-end

References:

PMID:25987607

Genotypes:

wdr8delta

FYPO:0004754 - abolished protein localization to mitotic spindle during mitosis

References:

PMID:25987607

Genotypes:

wdr8delta

FYPO:0001861 - increased minichromosome loss upon segregation during vegetative growth

References:

PMID:25987607

Genotypes:

wdr8delta

FYPO:0003787 - long mitotic spindle microtubules protruding beyond spindle pole body

References:

PMID:25987607

Genotypes:

wdr8delta

FYPO:0003788 - nuclear envelope protrusion present during mitosis

References:

PMID:25987607

Genotypes:

wdr8delta

FYPO:0006930 - sensitive to butylated hydroxyanisole

References:

PMID:30647105

Genotypes:

wdr8delta

FYPO:0000091 - sensitive to thiabendazole

References:

PMID:25987607

Genotypes:

wdr8delta

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

wdr8delta

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

PMID:23697806

Genotypes:

wdr8delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF00400	WD40	WD40_rpt	PFAM
PS50082	WD_REPEATS_2	WD40_rpt	PROSITE_PROFILES
PS50294	WD_REPEATS_REGION		PROSITE_PROFILES
SM00320	WD40_4	WD40_rpt	SMART
SSF50978	WD40 repeat-like	WD40_repeat_dom_sf	SUPERFAMILY
G3DSA:2.130.10.10		WD40/YVTN_repeat-like_dom_sf	GENE3D
PTHR16220	WD REPEAT PROTEIN 8-RELATED	Centrosome-WD_assoc	PANTHER

Orthologs

WRAP73 (HGNC:12759) Homo sapiens
wdr8 (SJAG_03062) Schizosaccharomyces japonicus

References / Literature

PMID:30463883 - Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe .: Yukawa M et al. G3 (Bethesda) 2019 Jan 09;9(1):269-280
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:25987607 - The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies.: Yukawa M et al. J Cell Biol 2015 May 25;209(4):549-62
PMID:22146723 - Systematic localization study on novel proteins encoded by meiotically up-regulated ORFs in fission yeast.: Ikebe C et al. Biosci Biotechnol Biochem 2011;75(12):2364-70
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.: Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.: Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.: Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;