protein coding gene - act1 (SPBC32H8.12c) - actin Act1

Gene summary

Standard name

act1

Systematic ID

SPBC32H8.12c

Product

actin Act1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

cps8, pi012

UniProt ID

P10989

ORFeome ID

50/50C12

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 1475485..1477333 reverse strand

Annotation

Protein features

Orthologs

• ACTA1 (HGNC:129) Homo sapiens
• ACTA2 (HGNC:130) Homo sapiens
• ACTC1 (HGNC:143) Homo sapiens
• ACTG1 (HGNC:144) Homo sapiens
• ACTG2 (HGNC:145) Homo sapiens
• act1 (SJAG_03145) Schizosaccharomyces japonicus
• ACT1 (YFL039C) Saccharomyces cerevisiae

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:24120641 - Dip1 defines a class of Arp2/3 complex activators that function without preformed actin filaments.

Wagner AR et al. Curr Biol 2013 Oct 21;23(20):1990-8

PMID:33378674 - The INO80 Complex Regulates Epigenetic Inheritance of Heterochromatin.

Shan CM et al. Cell Rep 2020 Dec 29;33(13):108561

PMID:23589458 - Insight into actin organization and function in cytokinesis from analysis of fission yeast mutants.

Subramanian D et al. Genetics 2013 Jun;194(2):435-46

PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.

Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697

PMID:21885283 - Distinct roles for F-BAR proteins Cdc15p and Bzz1p in actin polymerization at sites of endocytosis in fission yeast.

Arasada R et al. Curr Biol 2011 Sep 13;21(17):1450-9

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:12237855 - Genome-wide search of Schizosaccharomyces pombe genes causing overexpression-mediated cell cycle defects.

Tallada VA et al. Yeast 2002 Sep 30;19(13):1139-51

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:18346214 - btn1 affects endocytosis, polarization of sterol-rich membrane domains and polarized growth in Schizosaccharomyces pombe.

Codlin S et al. Traffic 2008 Jun;9(6):936-50

PMID:22891259 - Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission.

Cortés JC et al. J Cell Biol 2012 Aug 20;198(4):637-56

PMID:22905165 - The integrity of the cytokinesis machinery under stress conditions requires the glucan synthase Bgs1p and its regulator Cfh3p.

Sharifmoghadam MR et al. PLoS One 2012;7(8):e42726

PMID:25543282 - Profilin regulates F-actin network homeostasis by favoring formin over Arp2/3 complex.

Suarez C et al. Dev Cell 2015 Jan 12;32(1):43-53

PMID:30709916 - A Cdc42 GEF, Gef1, through endocytosis organizes F-BAR Cdc15 along the actomyosin ring and promotes concentric furrowing.

Onwubiko UN et al. J Cell Sci 2019 Feb 28;132(5)

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:19915592 - An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe.

Kim HS et al. Nat Struct Mol Biol 2009 Dec;16(12):1286-93

PMID:23755256 - The Sm complex is required for the processing of non-coding RNAs by the exosome.

Coy S et al. PLoS One 2013;8(6):e65606

PMID:19693008 - Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs.

Zofall M et al. Nature 2009 Sep 17;461(7262):419-22

PMID:9765059 - Subcellular localization and possible function of actin, tropomyosin and actin-related protein 3 (Arp3) in the fission yeast Schizosaccharomyces pombe.

Arai R et al. Eur J Cell Biol 1998 Aug;76(4):288-95

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.

Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7

PMID:9459302 - Identification of Myo3, a second type-II myosin heavy chain in the fission yeast Schizosaccharomyces pombe.

Motegi F et al. FEBS Lett 1997 Dec 29;420(2-3):161-6

PMID:15537703 - The nuclear kinase Lsk1p positively regulates the septation initiation network and promotes the successful completion of cytokinesis in response to perturbation of the actomyosin ring in Schizosaccharomyces pombe.

Karagiannis J et al. Mol Biol Cell 2005 Jan;16(1):358-71

PMID:24115772 - SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis.

Bohnert KA et al. Genes Dev 2013 Oct 01;27(19):2164-77

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:38718864 - H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe.

Qin B et al. J Biol Chem 2024 May 06;:107345

PMID:25451933 - Aip1 promotes actin filament severing by cofilin and regulates constriction of the cytokinetic contractile ring.

Chen Q et al. J Biol Chem 2015 Jan 23;290(4):2289-300

PMID:20587778 - Quantitative analysis of the mechanism of endocytic actin patch assembly and disassembly in fission yeast.

Sirotkin V et al. Mol Biol Cell 2010 Aug 15;21(16):2894-904

PMID:21148484 - Purification of actin from fission yeast Schizosaccharomyces pombe and characterization of functional differences from muscle actin.

Ti SC et al. J Biol Chem 2011 Feb 18;286(7):5784-92

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:32915139 - Stress-activated MAPK signaling controls fission yeast actomyosin ring integrity by modulating formin For3 levels.

Gómez-Gil E et al. Elife 2020 Sep 11;9

PMID:34613787 - Counting actin in contractile rings reveals novel contributions of cofilin and type II myosins to fission yeast cytokinesis.

Malla M et al. Mol Biol Cell 2022 May 15;33(6):ar51

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:23770677 - In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics.

Mishra M et al. Nat Cell Biol 2013 Jul;15(7):853-9

PMID:10526238 - Overproduction of elongation factor 1alpha, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast.

Suda M et al. Genes Cells 1999 Sep;4(9):517-27

PMID:21723825 - Cofilin-linked changes in actin filament flexibility promote severing.

McCullough BR et al. Biophys J 2011 Jul 06;101(1):151-9

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:19040720 - Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment.

Shevchenko A et al. Genome Biol 2008;9(11):R167

PMID:8207058 - The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis.

Balasubramanian MK et al. J Cell Biol 1994 Jun;125(6):1289-301

PMID:9573052 - Pom1p, a fission yeast protein kinase that provides positional information for both polarized growth and cytokinesis.

Bähler J et al. Genes Dev 1998 May 01;12(9):1356-70

PMID:9606213 - FH3, a domain found in formins, targets the fission yeast formin Fus1 to the projection tip during conjugation.

Petersen J et al. J Cell Biol 1998 Jun 01;141(5):1217-28

PMID:11056543 - Type II myosin regulatory light chain relieves auto-inhibition of myosin-heavy-chain function.

Naqvi NI et al. Nat Cell Biol 2000 Nov;2(11):855-8

PMID:9490631 - F-actin distribution and function during sexual differentiation in Schizosaccharomyces pombe.

Petersen J et al. J Cell Sci 1998 Apr;111 ( Pt 7):867-76

PMID:3309892 - A single intronless action gene in the fission yeast Schizosaccharomyces pombe: nucleotide sequence and transcripts formed in homologous and heterologous yeast.

Mertins P et al. Nucleic Acids Res 1987 Sep 25;15(18):7369-79

PMID:22426534 - DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.

Sabouri N et al. Genes Dev 2012 Mar 15;26(6):581-93

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Das J et al. Sci Signal 2013 May 21;6(276):ra38

PMID:24167549 - A molecular evolution approach to study the roles of tropomyosin in fission yeast.

Cranz-Mileva S et al. PLoS One 2013;8(10):e76726

PMID:20705466 - Fimbrin and tropomyosin competition regulates endocytosis and cytokinesis kinetics in fission yeast.

Skau CT et al. Curr Biol 2010 Aug 24;20(16):1415-22

PMID:16256112 - Stg 1 is a novel SM22/transgelin-like actin-modulating protein in fission yeast.

Nakano K et al. FEBS Lett 2005 Nov 21;579(28):6311-6

PMID:1524835 - Isolation and characterization of mutants supersensitive to the spindle poison, isopropyl N-3-chlorophenyl carbamate (CIPC) in the fission yeast Schizosaccharomyces pombe.

Ishiguro J et al. Jpn J Genet 1992 Apr;67(2):97-109

PMID:24634168 - Proteome-wide search for PP2A substrates in fission yeast.

Bernal M et al. Proteomics 2014 Jun;14(11):1367-80

PMID:8774852 - An actin point-mutation neighboring the 'hydrophobic plug' causes defects in the maintenance of cell polarity and septum organization in the fission yeast Schizosaccharomyces pombe.

Ishiguro J et al. FEBS Lett 1996 Sep 02;392(3):237-41

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:8978670 - Fission yeast Sop2p: a novel and evolutionarily conserved protein that interacts with Arp3p and modulates profilin function.

Balasubramanian MK et al. EMBO J 1996 Dec 02;15(23):6426-37

PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.

Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.

Wu JQ et al. Science 2005 Oct 14;310(5746):310-4

PMID:35811551 - Mitotic checkpoint gene expression is tuned by codon usage bias.

Esposito E et al. EMBO J 2022 Aug 01;41(15):e107896

PMID:18976909 - Genotype-phenotype correlations in ACTA1 mutations that cause congenital myopathies.

Feng JJ et al. Neuromuscul Disord 2009 Jan;19(1):6-16

PMID:17353264 - Efficient RNA polyuridylation by noncanonical poly(A) polymerases.

Rissland OS et al. Mol Cell Biol 2007 May;27(10):3612-24

PMID:10371213 - Identification of cold-sensitive mutations in the Schizosaccharomyces pombe actin locus.

McCollum D et al. FEBS Lett 1999 May 28;451(3):321-6

PMID:14534314 - Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro.

Spahr H et al. J Biol Chem 2003 Dec 19;278(51):51301-6

PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.

McDonald NA et al. Elife 2017 Sep 15;6

PMID:10547441 - Ultrastructure of cell wall of the cps8 actin mutant cell in Schizosaccharomyces pombe.

Ishijima SA et al. FEMS Microbiol Lett 1999 Nov 01;180(1):31-7

PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.

Nie M et al. Sci Rep 2015 Sep 25;5:14389

PMID:15743909 - Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast.

Kovar DR et al. Mol Biol Cell 2005 May;16(5):2313-24

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:22024167 - Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring.

Chen Q et al. J Cell Biol 2011 Oct 31;195(3):485-98

PMID:19910462 - Histone variant H2A.Z regulates centromere silencing and chromosome segregation in fission yeast.

Hou H et al. J Biol Chem 2010 Jan 15;285(3):1909-18

PMID:39156640 - Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.

Ramos M et al. iScience 2024 Aug 16;27(8):110477

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:28338873 - Molecular dissection of the actin-binding ability of the fission yeast α-actinin, Ain1, in vitro and in vivo.

Morita R et al. J Biochem 2017 Aug 01;162(2):93-102

PMID:18562692 - Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability.

Meadows JC et al. Mol Biol Cell 2008 Sep;19(9):3713-23

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:27501521 - Analysis of biophysical and functional consequences of tropomyosin-fluorescent protein fusions.

Brooker HR et al. FEBS Lett 2016 Sep;590(18):3111-21

PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.

Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99

PMID:28282023 - Competition between Tropomyosin, Fimbrin, and ADF/Cofilin drives their sorting to distinct actin filament networks.

Christensen JR et al. Elife 2017 Mar 10;6

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:41236477 - Actin arginylation alters myosin engagement and F-actin patterning despite structural conservation.

Pinto CS et al. J Cell Biol 2026 Jan 05;225(1)

PMID:17202724 - Localization of type I myosin and F-actin to the leading edge region of the forespore membrane in Schizosaccharomyces pombe.

Itadani A et al. Cell Struct Funct 2006;31(2):181-95

PMID:23148219 - Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.

Liu SL et al. J Biol Chem 2013 Jan 04;288(1):487-97

PMID:19430462 - Decapping is preceded by 3' uridylation in a novel pathway of bulk mRNA turnover.

Rissland OS et al. Nat Struct Mol Biol 2009 Jun;16(6):616-23

PMID:11694585 - Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton.

Nakano K et al. Mol Biol Cell 2001 Nov;12(11):3515-26

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:19911051 - The Schizosaccharomyces pombe JmjC-protein, Msc1, prevents H2A.Z localization in centromeric and subtelomeric chromatin domains.

Buchanan L et al. PLoS Genet 2009 Nov;5(11):e1000726

PMID:30155942 - Sfh1, an essential component of the RSC chromatin remodeling complex, maintains genome integrity in fission yeast.

Kotomura N et al. Genes Cells 2018 Sep;23(9):738-752

PMID:11294907 - Roles of a fimbrin and an alpha-actinin-like protein in fission yeast cell polarization and cytokinesis.

Wu JQ et al. Mol Biol Cell 2001 Apr;12(4):1061-77

PMID:16141239 - Endocytosis in fission yeast is spatially associated with the actin cytoskeleton during polarised cell growth and cytokinesis.

Gachet Y et al. J Cell Sci 2005 Sep 15;118(Pt 18):4231-42

PMID:19933844 - Fission yeast Iec1-ino80-mediated nucleosome eviction regulates nucleotide and phosphate metabolism.

Hogan CJ et al. Mol Cell Biol 2010 Feb;30(3):657-74

PMID:31748520 - Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in yeast.

Elías-Villalobos A et al. Nat Commun 2019 Nov 20;10(1):5237

PMID:11460168 - A MAP kinase-dependent actin checkpoint ensures proper spindle orientation in fission yeast.

Gachet Y et al. Nature 2001 Jul 19;412(6844):352-5

PMID:32313168 - Serine catabolism produces ROS, sensitizes cells to actin dysfunction, and suppresses cell growth in fission yeast.

Kanou A et al. J Antibiot (Tokyo) 2020 Aug;73(8):574-580

PMID:37531259 - Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins.

Willet AH et al. Mol Biol Cell 2023 Oct 01;34(11):br17

PMID:8978671 - The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin.

McCollum D et al. EMBO J 1996 Dec 02;15(23):6438-46

PMID:27075176 - The F-actin bundler α-actinin Ain1 is tailored for ring assembly and constriction during cytokinesis in fission yeast.

Li Y et al. Mol Biol Cell 2016 Jun 01;27(11):1821-33

PMID:28869610 - Mutations in ACTRT1 and its enhancer RNA elements lead to aberrant activation of Hedgehog signaling in inherited and sporadic basal cell carcinomas.

Bal E et al. Nat Med 2017 Oct;23(10):1226-1233

PMID:11112691 - Two type V myosins with non-overlapping functions in the fission yeast Schizosaccharomyces pombe: Myo52 is concerned with growth polarity and cytokinesis, Myo51 is a component of the cytokinetic actin ring.

Win TZ et al. J Cell Sci 2001 Jan;114(Pt 1):69-79

PMID:20434336 - The cortical ER network limits the permissive zone for actomyosin ring assembly.

Zhang D et al. Curr Biol 2010 Jun 08;20(11):1029-34

PMID:23028742 - The natural anticancer agent plumbagin induces potent cytotoxicity in MCF-7 human breast cancer cells by inhibiting a PI-5 kinase for ROS generation.

Lee JH et al. PLoS One 2012;7(9):e45023

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.

Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928

PMID:8292390 - A novel mutation involved in the mitotic checkpoint in the fission yeast Schizosaccharomyces pombe.

Ishiguro J et al. Jpn J Genet 1993 Aug;68(4):265-76

PMID:8972853 - Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed.

Javerzat JP et al. Nucleic Acids Res 1996 Dec 01;24(23):4676-83

PMID:24196839 - Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables.

Clayton JE et al. Mol Biol Cell 2014 Jan;25(1):66-75

PMID:25356590 - Multifaceted genome control by Set1 Dependent and Independent of H3K4 methylation and the Set1C/COMPASS complex.

Mikheyeva IV et al. PLoS Genet 2014 Oct;10(10):e1004740

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:24442611 - Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.

Chen W et al. RNA 2014 Mar;20(3):308-20

PMID:26542710 - Identification and characterization of Csh3 as an SH3 protein that interacts with fission yeast Cap1.

Yamamoto T et al. FEMS Yeast Res 2015 Dec;15(8)

PMID:28808035 - Fission yeast myosin Myo2 is down-regulated in actin affinity by light chain phosphorylation.

Pollard LW et al. Proc Natl Acad Sci U S A 2017 Aug 29;114(35):E7236-E7244

PMID:39358553 - Ageing-associated long non-coding RNA extends lifespan and reduces translation in non-dividing cells.

Anver S et al. EMBO Rep 2024 Oct 02;

PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86

PMID:21832151 - Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis.

Kashiwazaki J et al. Mol Biol Cell 2011 Oct;22(19):3658-70

PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.

Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671

PMID:15620689 - Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.

Mitsuzawa H et al. FEBS Lett 2005 Jan 03;579(1):48-52