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protein coding gene - mok12 (SPBC32H8.13c) - spore wall alpha-1,3-glucan synthase Mok12

Gene summary

Standard name
mok12
Systematic ID
SPBC32H8.13c
Product
spore wall alpha-1,3-glucan synthase Mok12
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPACTOKYO_453.25c
UniProt ID
Q9UUL4
ORFeome ID
49/49H11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1478007..1486100 reverse strand

Annotation

PBO:0002492 - 2.4.1.183

GO biological process

GO:0070591 - ascospore wall biogenesis

References:

GO:0070600 - fungal-type cell wall (1->3)-alpha-glucan biosynthetic process

References:

GO cellular component

GO:0005628 - prospore membrane

References:

GO molecular function

GO:0047657 - alpha-1,3-glucan synthase activity

References:

Multi-locus phenotype

FYPO:0002161 - normal growth on Calcofluor White

References:

Genotypes:

FYPO:0004892 - normal growth on echinocandin

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000015 - RNA absent

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000175 - abnormal ascospore wall assembly

References:

Genotypes:

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0000583 - abolished sporulation

References:

Genotypes:

FYPO:0007493 - decreased ascospore wall alpha-glucan level

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0007494 - increased ascospore wall beta-glucan level

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0003795 - normal cell wall alpha-glucan level

References:

Genotypes:

FYPO:0003797 - normal conjugation frequency

References:

Genotypes:

FYPO:0002161 - normal growth on Calcofluor White

References:

Genotypes:

FYPO:0004892 - normal growth on echinocandin

References:

Genotypes:

FYPO:0004927 - normal prospore membrane formation

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF00128Alpha-amylaseGH13_cat_domPfam
PF00534Glycos_transf_1Glyco_trans_1Pfam
PF08323Glyco_transf_5Starch_synth_cat_domPfam
PF26108GH_Mok13Mok11-13/Ags1-like_GHPfam
PF26111Ig_Mok13Mok11-13/Ags1-like_IgPfam
PF26114Ig_2_Mok13Mok11-13/Ags1-like_Ig_2Pfam
PF26122CBM_Mok13Mok11-13/Ags1-like_CBMPfam
PF2612712TM_Mok13Mok11-14/Ags1-like_TMPfam
PF26775HTH_Mok13HTH_Mok13Pfam
cd03791GT5_Glycogen_synthase_DULL1-likeCDD
SM00642AamyGH13_cat_domSMART
G3DSA:3.20.20.80:FF:000114CATH-FunFam
G3DSA:3.20.20.80:FF:000162CATH-FunFam
G3DSA:3.20.20.80CATH-Gene3D
G3DSA:3.40.50.2000CATH-Gene3D
SSF51445GH_hydrolase_sfSUPERFAMILY
SSF53756SUPERFAMILY
PTHR47182Mok11-14/Ags1-likePANTHER
Transmembrane alpha helixDeepTMHMM
Signal PeptideDeepTMHMM-Signal-Peptide
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
GO_REF:0000002 - Comments
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16420355 - Synthesis of alpha-glucans in fission yeast spores is carried out by three alpha-glucan synthase paralogues, Mok12p, Mok13p and Mok14p.
García I et al. Mol Microbiol 2006 Feb;59(3):836-53
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:10087262 - Fission yeast alpha-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function.
Katayama S et al. J Cell Biol 1999 Mar 22;144(6):1173-86
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650