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protein coding gene - fbh1 (SPBC336.01) - DNA helicase I, ubiquitin ligase F-box adaptor Fbh1

Gene summary

Standard name
fbh1
Systematic ID
SPBC336.01
Product
DNA helicase I, ubiquitin ligase F-box adaptor Fbh1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
fdh1, fdh
UniProt ID
Q9USU3
ORFeome ID
38/38H09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2733210..2736084 forward strand

Annotation

Catalytic activity attributes

PBO:0091799 - KM of ATP for ATPase activity 110 uM

References:

PBO:0018004 - Ssb1 stimulates helicase activity at low ATP concentration (0.2 mM)

References:

PBO:0018002 - unwinds duplexes up to 150 bp

References:

GO biological process

GO:0006310 - DNA recombination

References:

GO:0000724 - double-strand break repair via homologous recombination

References:

GO:1990426 - mitotic recombination-dependent replication fork processing

References:

GO:0000018 - regulation of DNA recombination

References:

GO:0031297 - replication fork processing

References:

GO:0000712 - resolution of meiotic recombination intermediates

References:

GO:0031146 - SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0043224 - nuclear SCF ubiquitin ligase complex

References:

GO:0005634 - nucleus

References:

GO:0017117 - single-stranded DNA-dependent ATP-dependent DNA helicase complex

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016787 - hydrolase activity

References:

GO:0005515 - protein binding

References:

GO:1990518 - single-stranded 3'-5' DNA helicase activity

References:

GO:0003697 - single-stranded DNA binding

References:

GO:1990756 - ubiquitin-like ligase-substrate adaptor activity

References:

Multi-locus phenotype

FYPO:0004159 - abnormal homologous chromosome segregation

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0003912 - decreased double-strand break repair via homologous recombination

References:

Genotypes:

FYPO:0003913 - decreased minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0000167 - increased DNA recombination at mitotic DNA replication fork barriers

References:

Genotypes:

FYPO:0006419 - increased duration of Rad51 focus presence during meiotic cell cycle

References:

Genotypes:

FYPO:0007102 - multiple spindles during meiosis II

References:

Genotypes:

FYPO:0001690 - normal growth on camptothecin

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0007106 - normal mitotic spindle morphology

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

Protein features

PBO:0111823 - F-box protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0004159 - abnormal homologous chromosome segregation

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003674 - abolished ATPase activity

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Genotypes:

FYPO:0001978 - bent mitotic spindle

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Genotypes:

FYPO:0007101 - bent spindle during meiosis I

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0003912 - decreased double-strand break repair via homologous recombination

References:

Genotypes:

FYPO:0002485 - decreased intergenic meiotic recombination

References:

Genotypes:

FYPO:0003913 - decreased minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0003286 - decreased mitotic chromosome condensation

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0000636 - increased cell population growth rate

References:

Genotypes:

FYPO:0000657 - increased DNA binding

References:

Genotypes:

FYPO:0000167 - increased DNA recombination at mitotic DNA replication fork barriers

References:

Genotypes:

FYPO:0007006 - increased DNA recombination downstream of mitotic DNA replication fork barriers

References:

Genotypes:

FYPO:0007105 - increased duration of Rad52 focus presence during meiotic cell cycle

References:

Genotypes:

FYPO:0003914 - increased protein level in stationary phase

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0007102 - multiple spindles during meiosis II

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0002642 - sensitive to amphotericin B

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0002641 - sensitive to micafungin

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0005889 - sensitive to sodium chloride

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0000678 - unequal homologous chromosome segregation

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Subunit composition

PBO:0001467 - monomeric

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00580UvrD-helicaseUvrD-like_ATP-bdPFAM
PF13361UvrD_CUvrD-like_CPFAM
cd17932DEXQc_UvrDCDD
PS50181FBOXF-box_domPROSITE_PROFILES
G3DSA:3.40.50.300:FF:005425FUNFAM
G3DSA:3.40.50.300:FF:005416FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR11070UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBERUvrD-likePANTHER

Orthologs

References / Literature

PMID:31149897 - The PCNA unloader Elg1 promotes recombination at collapsed replication forks in fission yeast.
Tamang S et al. Elife 2019 May 31;8
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.
Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:23738021 - A genome-wide screening of potential target genes to enhance the antifungal activity of micafungin in Schizosaccharomyces pombe.
Zhou X et al. PLoS One 2013;8(5):e65904
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22723423 - The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.
Lorenz A et al. Science 2012 Jun 22;336(6088):1585-8
PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:9228070 - A DNA helicase from Schizosaccharomyces pombe stimulated by single-stranded DNA-binding protein at low ATP concentration.
Park JS et al. J Biol Chem 1997 Jul 25;272(30):18910-9
PMID:16135800 - The F-Box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteins.
Osman F et al. Mol Cell Biol 2005 Sep;25(18):8084-96
PMID:27304859 - Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation.
Polakova S et al. PLoS Genet 2016 Jun;12(6):e1006102
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:17515930 - Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice.
Kai M et al. Nat Cell Biol 2007 Jun;9(6):691-7
GO_REF:0000002 - Comments
PMID:15147268 - Molecular interactions of fission yeast Skp1 and its role in the DNA damage checkpoint.
Lehmann A et al. Genes Cells 2004 May;9(5):367-82
PMID:21183410 - Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.
Vejrup-Hansen R et al. DNA Repair (Amst) 2011 Mar 07;10(3):283-95
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:19836238 - Stress-induced phosphorylation of S. pombe Atf1 abrogates its interaction with F box protein Fbh1.
Lawrence CL et al. Curr Biol 2009 Dec 01;19(22):1907-11
PMID:30667359 - Factors affecting template switch recombination associated with restarted DNA replication.
Jalan M et al. Elife 2019 Jan 22;8
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16135799 - Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates.
Morishita T et al. Mol Cell Biol 2005 Sep;25(18):8074-83
PMID:22292001 - SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.
Okamoto SY et al. PLoS One 2012;7(1):e30622
PMID:24741789 - Rad51 overexpression and resistance to genotoxic agents. A study in the fission yeast Schizosaccharomyces pombe.
Stanescu RS et al. Rev Med Chir Soc Med Nat Iasi 2014;118(1):133-40
PMID:25165823 - Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
Tsutsui Y et al. PLoS Genet 2014 Aug;10(8):e1004542
PMID:18312697 - Essential and distinct roles of the F-box and helicase domains of Fbh1 in DNA damage repair.
Sakaguchi C et al. BMC Mol Biol 2008 Mar 03;9:27
PMID:23263988 - Quantitative analysis of chromosome condensation in fission yeast.
Petrova B et al. Mol Cell Biol 2013 Mar;33(5):984-98
PMID:21149262 - A failure of meiotic chromosome segregation in a fbh1Delta mutant correlates with persistent Rad51-DNA associations.
Sun W et al. Nucleic Acids Res 2011 Mar;39(5):1718-31
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7