protein coding gene - cdc6 (SPBC336.04) - DNA polymerase delta catalytic subunit Cdc6

Gene summary

Standard name

cdc6

Systematic ID

SPBC336.04

Product

DNA polymerase delta catalytic subunit Cdc6

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

pol3, pold, mis10

UniProt ID

P30316

ORFeome ID

34/34A06

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 2743762..2748596 forward strand

Annotation

Protein features

Orthologs

• POLD1 (HGNC:9175) Homo sapiens
• cdc6 (SJAG_02818) Schizosaccharomyces japonicus
• POL3 (YDL102W) Saccharomyces cerevisiae

References / Literature

PMID:10671561 - Structure and activity associated with multiple forms of Schizosaccharomyces pombe DNA polymerase delta.

Zuo S et al. J Biol Chem 2000 Feb 18;275(7):5153-62

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:12124382 - The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta.

Bermudez VP et al. J Biol Chem 2002 Sep 27;277(39):36853-62

PMID:10704216 - In vitro reconstitution of the Schizosaccharomyces pombe alternative excision repair pathway.

Alleva JL et al. Biochemistry 2000 Mar 14;39(10):2659-66

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:34228709 - Expression of the cancer-associated DNA polymerase ε P286R in fission yeast leads to translesion synthesis polymerase dependent hypermutation and defective DNA replication.

Soriano I et al. PLoS Genet 2021 Jul;17(7):e1009526

PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.

Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20

PMID:17660542 - Mms22 preserves genomic integrity during DNA replication in Schizosaccharomyces pombe.

Dovey CL et al. Genetics 2007 Sep;177(1):47-61

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:8887553 - The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27.

MacNeill SA et al. EMBO J 1996 Sep 02;15(17):4613-28

PMID:9348105 - DNA replication and order of cell cycle events: a role for protein isoprenylation?

Galli I et al. Biol Chem 1997 Sep;378(9):963-73

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80

PMID:9407031 - Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1.

Saka Y et al. Genes Dev 1997 Dec 15;11(24):3387-400

PMID:958201 - Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe.

Nurse P et al. Mol Gen Genet 1976 Jul 23;146(2):167-78

PMID:22144917 - The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved.

Miyabe I et al. PLoS Genet 2011 Dec;7(12):e1002407

PMID:8443413 - Cell cycle expression of two replicative DNA polymerases alpha and delta from Schizosaccharomyces pombe.

Park H et al. Mol Biol Cell 1993 Feb;4(2):145-57

PMID:9070271 - PCNA and DNA polymerase delta catalytic subunit from Schizosaccharomyces pombe do not interact directly.

Tratner I et al. Biochem Biophys Res Commun 1997 Feb 13;231(2):321-8

PMID:12589755 - The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway.

Kanoh J et al. J Mol Biol 2003 Feb 28;326(4):1081-94

PMID:1960723 - Characterization of the POL3 gene product from Schizosaccharomyces pombe indicates inter-species conservation of the catalytic subunit of DNA polymerase delta.

Pignède G et al. J Mol Biol 1991 Nov 20;222(2):209-18

PMID:9693370 - Mutational effect of fission yeast polalpha on cell cycle events.

Bhaumik D et al. Mol Biol Cell 1998 Aug;9(8):2107-23

PMID:19686603 - Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis.

Sanchez Garcia J et al. BMC Mol Biol 2009 Aug 17;10:82

PMID:10888871 - Mitotic replication initiation proteins are not required for pre-meiotic S phase.

Forsburg SL et al. Nat Genet 2000 Jul;25(3):263-8

PMID:25664722 - A global profile of replicative polymerase usage.

Daigaku Y et al. Nat Struct Mol Biol 2015 Mar;22(3):192-198

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:9928931 - The S/M checkpoint at 37 degrees C and the recovery of viability of the mutant poldeltats3 require the crb2+/rhp9+ gene in fission yeast.

Grenon M et al. Mol Gen Genet 1999 Jan;260(6):522-34

PMID:15576681 - Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta.

Tanaka H et al. Nucleic Acids Res 2004;32(21):6367-77

PMID:10388806 - Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes.

Snaith HA et al. Genetics 1999 Jul;152(3):839-51

PMID:10748208 - Fidelity of eucaryotic DNA polymerase delta holoenzyme from Schizosaccharomyces pombe.

Chen X et al. J Biol Chem 2000 Jun 09;275(23):17677-82

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:33796794 - Increased expression of Polδ does not alter the canonical replication program in vivo .

Zach R et al. Wellcome Open Res 2021;6:44

PMID:10559981 - A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.

Edwards RJ et al. Nat Cell Biol 1999 Nov;1(7):393-8

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:9154809 - A novel mutant allele of Schizosaccharomyces pombe rad26 defective in monitoring S-phase progression to prevent premature mitosis.

Uchiyama M et al. Mol Cell Biol 1997 Jun;17(6):3103-15

GO_REF:0000002 - Comments

PMID:9108295 - The Schizosaccharomyces pombe cdc6 gene encodes the catalytic subunit of DNA polymerase delta.

Iino Y et al. Mol Gen Genet 1997 Mar 18;254(1):93-7

PMID:23349636 - Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.

Roseaulin LC et al. PLoS Genet 2013;9(1):e1003213

PMID:22645654 - Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.

Akai Y et al. Open Biol 2011 Dec;1(4):110023

PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063

PMID:24587136 - The oxidative stress responsive transcription factor Pap1 confers DNA damage resistance on checkpoint-deficient fission yeast cells.

Belfield C et al. PLoS One 2014;9(2):e89936

PMID:18180284 - Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stability.

Bailis JM et al. Mol Cell Biol 2008 Mar;28(5):1724-38

PMID:12697806 - Replication proteins influence the maintenance of telomere length and telomerase protein stability.

Dahlén M et al. Mol Cell Biol 2003 May;23(9):3031-42

PMID:16040599 - Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast.

Kim J et al. Nucleic Acids Res 2005;33(13):4078-89

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:15533944 - Interaction of checkpoint proteins Hus1/Rad1/Rad9 with DNA base excision repair enzyme MutY homolog in fission yeast, Schizosaccharomyces pombe.

Chang DY et al. J Biol Chem 2005 Jan 07;280(1):408-17

PMID:41525994 - Transcriptional PBR cycles at pericentromeric repeats cause gross chromosomal rearrangements through Rad52-dependent ADR-loop formation.

Xu R et al. Nucleic Acids Res 2026 Jan 05;54(1)

PMID:6828164 - Control of timing of cell cycle events in fission yeast by the wee 1+ gene.

Fantes PA Nature 1983 Mar 10;302(5904):153-5

PMID:9794798 - Mutant DNA polymerase delta from thermosensitive Schizosaccharomyces pombe strains display reduced stimulation by proliferating cell nuclear antigen.

Perderiset M et al. Biochem J 1998 Nov 01;335 ( Pt 3)(Pt 3):581-8

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PB_REF:0000003 - Disease Association Curation

PMID:7700230 - DNA polymerase delta is required for the replication feedback control of cell cycle progression in Schizosaccharomyces pombe.

Francesconi S et al. Mol Gen Genet 1995 Mar 10;246(5):561-9

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:10757807 - Cid1, a fission yeast protein required for S-M checkpoint control when DNA polymerase delta or epsilon is inactivated.

Wang SW et al. Mol Cell Biol 2000 May;20(9):3234-44

PMID:15173383 - The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit.

Sanchez Garcia J et al. Nucleic Acids Res 2004;32(10):3005-16

PMID:15367656 - Swi1 and Swi3 are components of a replication fork protection complex in fission yeast.

Noguchi E et al. Mol Cell Biol 2004 Oct;24(19):8342-55

PMID:18667534 - Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.

Yin L et al. Mol Biol Cell 2008 Oct;19(10):4374-82

PMID:19109429 - GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.

Pai CC et al. Mol Biol Cell 2009 Feb;20(4):1213-22

PMID:14739927 - Regulation of checkpoint kinases through dynamic interaction with Crb2.

Mochida S et al. EMBO J 2004 Jan 28;23(2):418-28

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:29092815 - The kinase domain residue serine 173 of S chizosaccharomyce s pombe Chk1 kinase is critical for the response to DNA replication stress.

Coulton N et al. Biol Open 2017 Dec 15;6(12):1840-1850

PMID:41330900 - PolySUMOylation of PCNA and Rad52 restricts centromeric recombination in fission yeast.

Markowska K et al. Nat Commun 2025 Dec 02;16(1):10837

PMID:8367300 - Fission yeast with DNA polymerase delta temperature-sensitive alleles exhibits cell division cycle phenotype.

Francesconi S et al. Nucleic Acids Res 1993 Aug 11;21(16):3821-8

PMID:9326594 - DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits.

Zuo S et al. Proc Natl Acad Sci U S A 1997 Oct 14;94(21):11244-9

PMID:9383050 - Mutational analysis of Cdc19p, a Schizosaccharomyces pombe MCM protein.

Forsburg SL et al. Genetics 1997 Nov;147(3):1025-41

PMID:17112379 - The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.

Locovei AM et al. Cell Div 2006 Nov 17;1:27

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:19205745 - Redundant roles of Srs2 helicase and replication checkpoint in survival and rDNA maintenance in Schizosaccharomyces pombe.

Yasuhira S Mol Genet Genomics 2009 May;281(5):497-509

PMID:1291234 - Expression of the catalytic subunits of pol alpha and pol delta from fission yeast Schizosaccharomyces pombe.

Pignède G et al. Chromosoma 1992;102(1 Suppl):S128-32

PMID:9799358 - Cdm1, the smallest subunit of DNA polymerase d in the fission yeast Schizosaccharomyces pombe, is non-essential for growth and division.

Reynolds N et al. Curr Genet 1998 Oct;34(4):250-8

PMID:11416129 - Schizosaccharomyces pombe cells lacking the amino-terminal catalytic domains of DNA polymerase epsilon are viable but require the DNA damage checkpoint control.

Feng W et al. Mol Cell Biol 2001 Jul;21(14):4495-504

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:16453724 - Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis.

Hirano T et al. EMBO J 1986 Nov;5(11):2973-9

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:7865880 - Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality.

Takahashi K et al. Mol Biol Cell 1994 Oct;5(10):1145-58

PMID:9135148 - p56(chk1) protein kinase is required for the DNA replication checkpoint at 37 degrees C in fission yeast.

Francesconi S et al. EMBO J 1997 Mar 17;16(6):1332-41

PMID:22540037 - Predicting the fission yeast protein interaction network.

Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67

PMID:9891047 - Mutator phenotype induced by aberrant replication.

Liu VF et al. Mol Cell Biol 1999 Feb;19(2):1126-35

PMID:15302919 - Genetic and biochemical analyses of Pfh1 DNA helicase function in fission yeast.

Ryu GH et al. Nucleic Acids Res 2004;32(14):4205-16

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:28481910 - PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.

Daigaku Y et al. PLoS Genet 2017 May;13(5):e1006789

PMID:26436826 - Polymerase δ replicates both strands after homologous recombination-dependent fork restart.

Miyabe I et al. Nat Struct Mol Biol 2015 Nov;22(11):932-8

PMID:31719112 - Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.

Kim SM et al. G3 (Bethesda) 2020 Jan 07;10(1):255-266

PMID:11073977 - Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1.

Boddy MN et al. Mol Cell Biol 2000 Dec;20(23):8758-66

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36