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protein coding gene - rnh1 (SPBC336.06c) - ribonuclease H Rnh1

Gene summary

Standard name
rnh1
Systematic ID
SPBC336.06c
Product
ribonuclease H Rnh1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UST8
ORFeome ID
32/32B12
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome II: 2748489..2750022 reverse strand

Annotation

PBO:0001451 - 3.1.26.4

Disease association

MONDO:0005181 - progressive external ophthalmoplegia

References:

MONDO:0014656 - progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2

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GO biological process

GO:0043137 - DNA replication, removal of RNA primer

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GO:0006264 - mitochondrial DNA replication

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GO:1990505 - mitotic DNA replication maintenance of fidelity

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GO:0006401 - RNA catabolic process

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GO cellular component

GO:0000785 - chromatin

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GO:0005739 - mitochondrion

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GO:0005730 - nucleolus

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GO:0005634 - nucleus

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GO molecular function

GO:0000287 - magnesium ion binding

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GO:0004523 - RNA-DNA hybrid ribonuclease activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0006321 - decreased protein localization to chromatin at stalled replication fork

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001742 - increased isochromosome formation

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Genotypes:

FYPO:0007936 - increased number of DNA breakpoint junctions

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Genotypes:

FYPO:0007227 - increased number of R-loops at centromere

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Genotypes:

FYPO:0004822 - increased number of R-loops at rDNA

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Genotypes:

FYPO:0006495 - increased number of R-loops at tRNA genes

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Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

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Genotypes:

FYPO:0003009 - increased protein localization to centromere outer repeat

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Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0006507 - increased subtelomeric DNA amplification during G0

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0002059 - inviable cell population

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Genotypes:

FYPO:0000256 - mutator

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Genotypes:

FYPO:0005398 - normal frequency of double-strand break repair via homologous recombination

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Genotypes:

FYPO:0004229 - normal growth during cellular response to ionizing radiation

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000472 - normal mating type switching

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0003481 - viable elongated vegetative cell, elongated upon mitotic entry

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0003217 - decreased chromatin silencing at centromere central core

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Genotypes:

FYPO:0004313 - decreased protein localization to CENP-A containing chromatin

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0005315 - increased chromatin silencing at centromere central core

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Genotypes:

FYPO:0004033 - increased protein localization to chromatin at tRNA genes

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Genotypes:

FYPO:0001690 - normal growth on camptothecin

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Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

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Genotypes:

FYPO:0005680 - normal number of Rad52 foci during mitotic S phase

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00075RNase_HRNaseH_domainPfam
PF01693Cauli_VIRNase_H1_NPfam
cd09280RNase_HI_eukaryote_likeCDD
PS50879RNASE_H_1RNaseH_domainPROSITE profiles
G3DSA:3.40.970.10:FF:000002CATH-FunFam
G3DSA:3.30.420.10RNaseH_sfCATH-Gene3D
G3DSA:3.40.970.10RNase_H1_N_sfCATH-Gene3D
SSF53098RNaseH-like_sfSUPERFAMILY
SSF55658Ribosomal_bL9/RNase_H1_NSUPERFAMILY
PTHR10642RNase_HPANTHER
PIRSF036852Ribonuclease_H1_eukRNase_H1_eukPIRSF
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

PMID:41525994 - Transcriptional PBR cycles at pericentromeric repeats cause gross chromosomal rearrangements through Rad52-dependent ADR-loop formation.
Xu R et al. Nucleic Acids Res 2026 Jan 05;54(1)
PMID:28717002 - Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.
Parajuli S et al. J Biol Chem 2017 Sep 15;292(37):15216-15224
PMID:29622660 - RNase H eliminates R-loops that disrupt DNA replication but is nonessential for efficient DSB repair.
Zhao H et al. EMBO Rep 2018 May;19(5)
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:29844133 - Loss of Elongation-Like Factor 1 Spontaneously Induces Diverse, RNase H-Related Suppressor Mutations in Schizosaccharomyces pombe .
Marayati BF et al. Genetics 2018 Aug;209(4):967-981
PMID:31294478 - Senataxin homologue Sen1 is required for efficient termination of RNA polymerase III transcription.
Rivosecchi J et al. EMBO J 2019 Aug 15;38(16):e101955
PMID:34464389 - R-loops and regulatory changes in chronologically ageing fission yeast cells drive non-random patterns of genome rearrangements.
Ellis DA et al. PLoS Genet 2021 Aug;17(8):e1009784
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000002 - Comments
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:29167439 - Eroded telomeres are rearranged in quiescent fission yeast cells through duplications of subtelomeric sequences.
Maestroni L et al. Nat Commun 2017 Nov 22;8(1):1684
PMID:27881299 - Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair.
Ohle C et al. Cell 2016 Nov 03;167(4):1001-1013.e7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:42231506 - Dbp7 interacts with RNA exosome component Dis3 to mediate CENP-A loading to centromeres.
Gao J et al. Genome Biol 2026 Jun 02;
PMID:33568651 - Replication dynamics of recombination-dependent replication forks.
Naiman K et al. Nat Commun 2021 Feb 10;12(1):923
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PB_REF:0000003 - Disease Association Curation
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:25392932 - RNA processing factors Swd2.2 and Sen1 antagonize RNA Pol III-dependent transcription and the localization of condensin at Pol III genes.
Legros P et al. PLoS Genet 2014 Nov;10(11):e1004794
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623