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protein coding gene - ecm14 (SPBC337.07c) - pseudopeptidase Ecm14

Gene summary

Standard name
ecm14
Systematic ID
SPBC337.07c
Product
pseudopeptidase Ecm14
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ppp28
UniProt ID
O74818
ORFeome ID
34/34H05
Characterisation status
conserved unknown
Feature type
mRNA gene
Genomic location
chromosome II: 1042110..1044094 reverse strand

Annotation

GO cellular component

GO:0005576 - extracellular region

References:

GO molecular function

GO:0008270 - zinc ion binding

References:

GO:0004181 - metallocarboxypeptidase activity

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Modification

MOD:00689 - disulfide crosslinked residues

References:

MOD:00006 - N-glycosylated residue

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MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

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SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00246Peptidase_M14Peptidase_M14PFAM
cd03860M14_CP_A-B_likeCDD
PS00132CARBOXYPEPT_ZN_1CARBOXYPEPT_ZN_1PROSITE_PATTERNS
PS52035PEPTIDASE_M14Peptidase_M14PROSITE_PROFILES
SM00631zn_carbPeptidase_M14SMART
PR00765CRBOXYPTASEAPeptidase_M14PRINTS
G3DSA:3.40.630.10:FF:000060FUNFAM
SSF53187Zn-dependent exopeptidasesSUPERFAMILY
G3DSA:3.40.630.10Zn peptidasesGENE3D
PTHR11705PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,BPANTHER

Orthologs

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.
Idiris A et al. Yeast 2006 Jan 30;23(2):83-99
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105