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protein coding gene - gtr1 (SPBC337.13c) - RagA/B GTPase Gtr1

Gene summary

Standard name
gtr1
Systematic ID
SPBC337.13c
Product
RagA/B GTPase Gtr1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74824
ORFeome ID
12/12H10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1053622..1054931 reverse strand

Annotation

GO biological process

GO:0009267 - cellular response to starvation

References:

GO:0010507 - negative regulation of autophagy

References:

GO:0110045 - negative regulation of cell cycle switching, mitotic to meiotic cell cycle

References:

GO:1904262 - negative regulation of TORC1 signaling

References:

GO:1904263 - positive regulation of TORC1 signaling

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0000329 - fungal-type vacuole membrane

References:

GO:1990131 - Gtr1-Gtr2 GTPase complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005525 - GTP binding

References:

GO:0003924 - GTPase activity

References:

GO:0043539 - protein serine/threonine kinase activator activity

References:

Multi-locus phenotype

FYPO:0004482 - abnormal vacuole fusion during vegetative growth

References:

Genotypes:

FYPO:0003252 - abolished cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0004457 - decreased protein localization to nucleus during nitrogen starvation

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0006345 - increased duration of protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0001043 - increased mating efficiency

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000243 - normal growth on proline nitrogen source

References:

Genotypes:

FYPO:0002672 - normal growth on rapamycin

References:

Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

References:

Genotypes:

FYPO:0005035 - normal protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001029 - resistance to canavanine

References:

Genotypes:

FYPO:0000280 - sterile

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002128 - abolished protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth

References:

Genotypes:

FYPO:0004250 - abolished protein localization to vacuolar membrane

References:

Genotypes:

FYPO:0001575 - abolished vegetative cell population growth

References:

Genotypes:

FYPO:0000046 - decreased cell population growth

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0010009 - decreased protein localization to cell surface, with protein mislocalized to cytoplasm

References:

Genotypes:

FYPO:0004457 - decreased protein localization to nucleus during nitrogen starvation

References:

Genotypes:

FYPO:0004780 - decreased protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0006345 - increased duration of protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0001043 - increased mating efficiency

References:

Genotypes:

FYPO:0002681 - increased protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0003031 - mating without nitrogen starvation

References:

Genotypes:

FYPO:0001522 - normal growth on caffeine

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0001545 - normal growth on L-canavanine

References:

Genotypes:

FYPO:0002672 - normal growth on rapamycin

References:

Genotypes:

FYPO:0008372 - normal protein localization to cell surface during vegetative growth

References:

Genotypes:

FYPO:0004248 - normal protein localization to vacuolar membrane

References:

Genotypes:

FYPO:0005035 - normal protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0002716 - normal vacuole fusion during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0001029 - resistance to canavanine

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000099 - sensitive to canavanine

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04670Gtr1_RagAGtr1_RagAPFAM
cd11384RagA_likeRagA/BCDD
G3DSA:3.40.50.300:FF:003828FUNFAM
G3DSA:3.30.450.190:FF:000002FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:3.30.450.190GENE3D
PTHR11259RAS-RELATED GTP BINDING RAG/GTR YEASTGtr1_RagAPANTHER

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23934889 - TORC1 signaling is governed by two negative regulators in fission yeast.
Ma N et al. Genetics 2013 Oct;195(2):457-68
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22344254 - The Vam6 and Gtr1-Gtr2 pathway activates TORC1 in response to amino acids in fission yeast.
Valbuena N et al. J Cell Sci 2012 Apr 15;125(Pt 8):1920-8
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:24344203 - Isp7 is a novel regulator of amino acid uptake in the TOR signaling pathway.
Laor D et al. Mol Cell Biol 2014 Mar;34(5):794-806
PMID:29330317 - tRNA production links nutrient conditions to the onset of sexual differentiation through the TORC1 pathway.
Otsubo Y et al. EMBO Rep 2018 Mar;19(3)
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:31641022 - The cytosolic form of aspartate aminotransferase is required for full activation of TOR complex 1 in fission yeast.
Reidman S et al. J Biol Chem 2019 Nov 29;294(48):18244-18255
PMID:34028542 - TORC2 inhibition of α-arrestin Aly3 mediates cell surface persistence of S. pombe Ght5 glucose transporter in low glucose.
Toyoda Y et al. J Cell Sci 2021 May 15;134(10)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:39010328 - Fission yeast Pib2 localizes to vacuolar membranes and regulates TOR complex 1 through evolutionarily conserved domains.
Morozumi Y et al. FEBS Lett 2024 Jul 15;
PMID:26689777 - Tor Signaling Regulates Transcription of Amino Acid Permeases through a GATA Transcription Factor Gaf1 in Fission Yeast.
Ma Y et al. PLoS One 2015;10(12):e0144677
PMID:29199950 - Ragulator and GATOR1 complexes promote fission yeast growth by attenuating TOR complex 1 through Rag GTPases.
Chia KH et al. Elife 2017 Dec 04;6
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:33534698 - Tripartite suppression of fission yeast TORC1 signaling by the GATOR1-Sea3 complex, the TSC complex, and Gcn2 kinase.
Fukuda T et al. Elife 2021 Feb 03;10
PMID:27227887 - The Loss of Lam2 and Npr2-Npr3 Diminishes the Vacuolar Localization of Gtr1-Gtr2 and Disinhibits TORC1 Activity in Fission Yeast.
Ma N et al. PLoS One 2016;11(5):e0156239
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
GO_REF:0000033 - Annotation inferences using phylogenetic trees