protein coding gene - rpb4 (SPBC337.14) - DNA-directed RNA polymerase II complex subunit Rpb4

Gene summary

Standard name

rpb4

Systematic ID

SPBC337.14

Product

DNA-directed RNA polymerase II complex subunit Rpb4

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O74825

ORFeome ID

39/39H10

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 1055509..1056502 forward strand

Annotation

Protein features

Orthologs

• POLR2D (HGNC:9191) Homo sapiens
• rpb4 (SJAG_00458) Schizosaccharomyces japonicus
• RPB4 (YJL140W) Saccharomyces cerevisiae

References / Literature

PMID:26792892 - Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.

Suzuki S et al. Nucleic Acids Res 2016 May 19;44(9):4147-62

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:28476936 - Genome-wide screen for cell growth regulators in fission yeast.

Weston L et al. J Cell Sci 2017 Jun 15;130(12):2049-2055

PMID:11839823 - Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.

Kimura M et al. Mol Cell Biol 2002 Mar;22(5):1577-88

PMID:30282034 - Elongation/Termination Factor Exchange Mediated by PP1 Phosphatase Orchestrates Transcription Termination.

Kecman T et al. Cell Rep 2018 Oct 02;25(1):259-269.e5

PMID:15620689 - Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.

Mitsuzawa H et al. FEBS Lett 2005 Jan 03;579(1):48-52

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:30862564 - A comparative study of the proteome regulated by the Rpb4 and Rpb7 subunits of RNA polymerase II in fission yeast.

Kumar D et al. J Proteomics 2019 May 15;199:77-88

PMID:19720063 - The Med8 mediator subunit interacts with the Rpb4 subunit of RNA polymerase II and Ace2 transcriptional activator in Schizosaccharomyces pombe.

Mehta S et al. FEBS Lett 2009 Oct 06;583(19):3115-20

PMID:28241144 - Mediator structure and rearrangements required for holoenzyme formation.

Tsai KL et al. Nature 2017 Apr 13;544(7649):196-201

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:10523639 - The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase II is essential for cell viability and similar in structure to the corresponding subunits of higher eukaryotes.

Sakurai H et al. Mol Cell Biol 1999 Nov;19(11):7511-8

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.

Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:10648788 - Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II.

Kimura M et al. Nucleic Acids Res 2000 Feb 15;28(4):952-9

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:24583182 - A novel method for purification of the endogenously expressed fission yeast Set2 complex.

Suzuki S et al. Protein Expr Purif 2014 May;97:44-9

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:11168400 - Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe.

Kimura M et al. Eur J Biochem 2001 Feb;268(3):612-9

PMID:11918671 - Level of the RNA polymerase II in the fission yeast stays constant but phosphorylation of its carboxyl terminal domain varies depending on the phase and rate of cell growth.

Sakurai H et al. Genes Cells 2002 Mar;7(3):273-84

PMID:15743411 - Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation.

Hayashi K et al. Genes Cells 2005 Mar;10(3):207-24

PMID:19458260 - Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.

Spåhr H et al. Proc Natl Acad Sci U S A 2009 Jun 09;106(23):9185-90

PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.

Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7

PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119

PMID:16972065 - The fission yeast Rpb4 subunit of RNA polymerase II plays a specialized role in cell separation.

Sharma N et al. Mol Genet Genomics 2006 Dec;276(6):545-54

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:15591044 - Structures of complete RNA polymerase II and its subcomplex, Rpb4/7.

Armache KJ et al. J Biol Chem 2005 Feb 25;280(8):7131-4

PMID:16204182 - RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.

Djupedal I et al. Genes Dev 2005 Oct 01;19(19):2301-6

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105