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protein coding gene - rga6 (SPBC354.13) - RhoGAP for Cdc42, Rga6

Gene summary

Standard name
rga6
Systematic ID
SPBC354.13
Product
RhoGAP for Cdc42, Rga6
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O43027
ORFeome ID
37/37H09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 581145..583861 forward strand

Annotation

GO biological process

GO:0032878 - regulation of establishment or maintenance of cell polarity

References:

GO:0007165 - signal transduction

References:

GO cellular component

GO:0032153 - cell division site

References:

GO:0097575 - lateral cell cortex

References:

GO:0035839 - non-growing cell tip

References:

GO molecular function

GO:0005096 - GTPase activator activity

References:

GO:0031267 - small GTPase binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0007682 - abnormal cortical polarity patch dynamics during mating

References:

Genotypes:

FYPO:0000303 - decreased conjugation frequency

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0008075 - increased protein localization to cell cortex of cell tip during vegetative growth

References:

Genotypes:

FYPO:0006636 - mislocalized protein distributed in cell cortex

References:

Genotypes:

FYPO:0000129 - spherical vegetative cell

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Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

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Genotypes:

FYPO:0002106 - viable stubby vegetative cell

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0007285 - abnormal dynamic protein localization to cell tip

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0005501 - abolished protein localization to cell cortex, with protein mislocalized to cytoplasm, during vegetative growth

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0001529 - decreased GTP binding

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Genotypes:

FYPO:0007707 - decreased GTPase activator activity

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0006637 - decreased protein localization to cell cortex of cell tip, with protein distributed in cortex

References:

Genotypes:

FYPO:0002871 - decreased protein localization to growing cell tip

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003532 - increased monopolar index

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0006874 - increased protein localization to cell cortex of old growing cell tip

References:

Genotypes:

FYPO:0002482 - inviable spheroid vegetative cell

References:

Genotypes:

FYPO:0007283 - normal protein localization to lateral cell cortex during vegetative growth

References:

Genotypes:

FYPO:0007284 - normal protein localization to non-growing cell tip

References:

Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0007282 - viable branched, swollen vegetative cell

References:

Genotypes:

FYPO:0002903 - viable pear-shaped vegetative cell

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0002110 - viable tapered vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

FYPO:0002176 - viable vegetative cell with normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0000110 - orthologs cannot be distinguished

Protein features

IDNameInterPro nameDB name
PF00620RhoGAPRhoGAP_domPfam
cd00159RhoGAPCDD
PS50238RHOGAPRhoGAP_domPROSITE profiles
SM00324RhoGAPRhoGAP_domSMART
G3DSA:1.10.555.10Rho_GTPase_activation_protCATH-Gene3D
SSF48350Rho_GTPase_activation_protSUPERFAMILY
PTHR23176Rho-GAPPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39012625 - Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.
Harrell MA et al. J Cell Biol 2024 Sep 02;223(10)
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:11737264 - Characterization of GTPase-activating proteins for the function of the Rho-family small GTPases in the fission yeast Schizosaccharomyces pombe.
Nakano K et al. Genes Cells 2001 Dec;6(12):1031-42
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:39333500 - Cdc42 mobility and membrane flows regulate fission yeast cell shape and survival.
Rutkowski DM et al. Nat Commun 2024 Sep 27;15(1):8363
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:34382996 - Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion.
Muriel O et al. J Cell Biol 2021 Oct 04;220(10)
PMID:34731607 - Stress-dependent inhibition of polarized cell growth through unbalancing the GEF/GAP regulation of Cdc42.
Salat-Canela C et al. Cell Rep 2021 Nov 02;37(5):109951
PMID:20164182 - Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein.
Soto T et al. J Biol Chem 2010 Apr 09;285(15):11516-25
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:26960792 - Rga6 is a Fission Yeast Rho GAP Involved in Cdc42 Regulation of Polarized Growth.
Revilla-Guarinos MT et al. Mol Biol Cell 2016 Mar 09;27(9):1524-35
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:18793338 - Rga2 is a Rho2 GAP that regulates morphogenesis and cell integrity in S. pombe.
Villar-Tajadura MA et al. Mol Microbiol 2008 Nov;70(4):867-81
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:30279276 - Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.
Gallo Castro D et al. J Cell Biol 2018 Dec 03;217(12):4215-4229
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:28821619 - Dsc E3 ligase localization to the Golgi requires the ATPase Cdc48 and cofactor Ufd1 for activation of sterol regulatory element-binding protein in fission yeast.
Burr R et al. J Biol Chem 2017 Sep 29;292(39):16333-16350
PMID:35048989 - The Cdc42 GTPase-activating protein Rga6 promotes the cortical localization of septin.
Zheng S et al. J Cell Sci 2022 Feb 15;135(4)