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protein coding gene - gcn2 (SPBC36B7.09) - eIF2 alpha kinase Gcn2

Gene summary

Standard name
gcn2
Systematic ID
SPBC36B7.09
Product
eIF2 alpha kinase Gcn2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ppk28, SPBP18G5.01
UniProt ID
Q9HGN1
ORFeome ID
50/50B01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2396432..2401552 forward strand

Annotation

Comment

PBO:0000206 - deletion mutant expression profiling

References:

Disease association

MONDO:0009937 - pulmonary venoocclusive disease

References:

MONDO:0009329 - pulmonary venoocclusive disease 2

References:

GO biological process

GO:0034198 - cellular response to amino acid starvation

References:

GO:0070314 - G1 to G0 transition

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GO:0140469 - GCN2-mediated signaling

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GO:0031571 - mitotic G1 DNA damage checkpoint signaling

References:

GO:1904689 - negative regulation of cytoplasmic translational initiation

References:

GO:1990625 - negative regulation of cytoplasmic translational initiation in response to stress

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GO:1904262 - negative regulation of TORC1 signaling

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GO:0010508 - positive regulation of autophagy

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0004694 - eukaryotic translation initiation factor 2alpha kinase activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0006295 - abolished macroautophagy during nitrogen starvation

References:

Genotypes:

FYPO:0002130 - abolished protein phosphorylation during cellular response to heat

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Genotypes:

FYPO:0006232 - abolished protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

FYPO:0000962 - normal growth on hydrogen peroxide

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Genotypes:

FYPO:0002350 - normal stress granule assembly during vegetative growth

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Genotypes:

FYPO:0001986 - resistance to 5-fluorouracil

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

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Genotypes:

Protein features

PBO:0111841 - RWD domain

Qualitative gene expression

PomGeneEx:0000021 - protein present

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004254 - abnormal mitotic cell cycle regulation during cellular response to UV

References:

Genotypes:

FYPO:0005179 - abnormal mitotic cell cycle regulation during cellular response to UV during mitotic G1 phase

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Genotypes:

FYPO:0006474 - abnormal regulation of translation in response to amino acid starvation

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Genotypes:

FYPO:0005043 - abnormal regulation of translation in response to UV

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Genotypes:

FYPO:0006444 - abnormal transcriptional response to amino acid starvation

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Genotypes:

FYPO:0007803 - abolished macroautophagy during leucine starvation

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Genotypes:

FYPO:0003020 - abolished protein autophosphorylation during vegetative growth

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Genotypes:

FYPO:0002898 - abolished protein phosphorylation during cellular response to DNA damage

References:

Genotypes:

FYPO:0006232 - abolished protein phosphorylation during cellular response to hydrogen peroxide

References:

Genotypes:

FYPO:0006398 - abolished protein phosphorylation during cellular response to leucine starvation

References:

Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0003298 - decreased protein autophosphorylation during vegetative growth

References:

Genotypes:

FYPO:0002446 - decreased protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0002993 - decreased protein phosphorylation during cellular response to methyl methanesulfonate

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Genotypes:

FYPO:0004228 - decreased protein phosphorylation during cellular response to UV

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Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

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Genotypes:

FYPO:0000712 - delayed onset of cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

References:

Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006294 - normal macroautophagy during nitrogen starvation

References:

Genotypes:

FYPO:0002996 - normal negative regulation of DNA replication during cellular response to hydrogen peroxide

References:

Genotypes:

FYPO:0002997 - normal negative regulation of DNA replication during cellular response to methyl methanesulfonate

References:

Genotypes:

FYPO:0005214 - normal regulation of translation in response to osmotic stress

References:

Genotypes:

FYPO:0005230 - normal regulation of translation in response to UV

References:

Genotypes:

FYPO:0002991 - premature mitotic G1/S phase transition during cellular response to hydrogen peroxide

References:

Genotypes:

FYPO:0003902 - resistance to etoposide

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0003491 - sensitive to UV during mitotic G1 phase

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF05773RWDRWD_domPFAM
PF12745HGTP_anticodon2HisRS-related_domPFAM
PF13393tRNA-synt_HisHisRS-like_corePFAM
PF00069PkinaseProt_kinase_domPFAM
cd14046STKc_EIF2AK4_GCN2_rpt2CDD
cd14012PK_eIF2AK_GCN2_rpt1CDD
cd23823RWD_GCN2CDD
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE_PATTERNS
PS50908RWDRWD_domPROSITE_PROFILES
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
SM00591RWD2001bRWD_domSMART
SM00220serkin_6Prot_kinase_domSMART
G3DSA:3.30.930.10:FF:000074FUNFAM
G3DSA:3.40.50.800:FF:000009FUNFAM
G3DSA:3.30.200.20:FF:000379FUNFAM
G3DSA:1.10.510.10:FF:001061FUNFAM
G3DSA:3.10.110.10:FF:000050FUNFAM
SSF55681Class II aaRS and biotin synthetasesaa-tRNA-synth_II/BPL/LPLSUPERFAMILY
SSF54495UBC-likeUBQ-conjugating_enzyme/RWDSUPERFAMILY
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:3.40.50.800Anticodon-bd_dom_sfGENE3D
G3DSA:3.10.110.10Ubiquitin Conjugating EnzymeUBQ-conjugating_enzyme/RWDGENE3D
G3DSA:3.30.200.20Phosphorylase Kinase; domain 1GENE3D
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
G3DSA:3.30.930.10Bira Bifunctional Protein; Domain 2aa-tRNA-synth_II/BPL/LPLGENE3D
PTHR11042EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATEDCC_SR_KinasePANTHER
PIRSF000660STPK_GCN2Gcn2PIRSF
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:38295128 - A connection between the ribosome and two S. pombe tRNA modification mutants subject to rapid tRNA decay.
De Zoysa T et al. PLoS Genet 2024 Jan 31;20(1):e1011146
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:20547592 - Vgl1, a multi-KH domain protein, is a novel component of the fission yeast stress granules required for cell survival under thermal stress.
Wen WL et al. Nucleic Acids Res 2010 Oct;38(19):6555-66
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21098141 - Cellular stress induces cytoplasmic RNA granules in fission yeast.
Nilsson D et al. RNA 2011 Jan;17(1):120-33
PMID:20444689 - Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth.
Shirai A et al. J Biol Chem 2010 Jul 16;285(29):22448-60
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:34085593 - Multiplexed suppression of TOR complex 1 induces autophagy during starvation.
Fukuda T et al. Autophagy 2021 Jul;17(7):1794-1795
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23671279 - Receptor for activated C-kinase (RACK1) homolog Cpc2 facilitates the general amino acid control response through Gcn2 kinase in fission yeast.
Tarumoto Y et al. J Biol Chem 2013 Jun 28;288(26):19260-8
PMID:23108671 - Fission yeast TORC1 prevents eIF2α phosphorylation in response to nitrogen and amino acids via Gcn2 kinase.
Valbuena N et al. J Cell Sci 2012 Dec 15;125(Pt 24):5955-9
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:17952063 - TOR signalling regulates mitotic commitment through the stress MAP kinase pathway and the Polo and Cdc2 kinases.
Petersen J et al. Nat Cell Biol 2007 Nov;9(11):1263-72
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:24280780 - Crosstalk between the Tor and Gcn2 pathways in response to different stresses.
Rødland GE et al. Cell Cycle 2014;13(3):453-61
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:33534698 - Tripartite suppression of fission yeast TORC1 signaling by the GATOR1-Sea3 complex, the TSC complex, and Gcn2 kinase.
Fukuda T et al. Elife 2021 Feb 03;10
PB_REF:0000003 - Disease Association Curation
PMID:16278445 - Stress-activated protein kinase pathway functions to support protein synthesis and translational adaptation in response to environmental stress in fission yeast.
Dunand-Sauthier I et al. Eukaryot Cell 2005 Nov;4(11):1785-93
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:28771613 - Activation of Gcn2 in response to different stresses.
Anda S et al. PLoS One 2017;12(8):e0182143
PMID:33970532 - Magnesium depletion extends fission yeast lifespan via general amino acid control activation.
Ohtsuka H et al. Microbiologyopen 2021 Mar;10(2):e1176
PMID:19033384 - The G1-S checkpoint in fission yeast is not a general DNA damage checkpoint.
Krohn M et al. J Cell Sci 2008 Dec 15;121(Pt 24):4047-54
PMID:32841241 - Hypomodified tRNA in evolutionarily distant yeasts can trigger rapid tRNA decay to activate the general amino acid control response, but with different consequences.
De Zoysa T et al. PLoS Genet 2020 Aug;16(8):e1008893
PMID:24333428 - Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.
Cambiaghi TD et al. Biochem Biophys Res Commun 2014 Jan 10;443(2):592-7
PMID:26493332 - Stress-induced inhibition of translation independently of eIF2α phosphorylation.
Knutsen JH et al. J Cell Sci 2015 Dec 01;128(23):4420-7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:18502752 - Int6/eIF3e promotes general translation and Atf1 abundance to modulate Sty1 MAPK-dependent stress response in fission yeast.
Udagawa T et al. J Biol Chem 2008 Aug 08;283(32):22063-75
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:17369398 - A novel checkpoint mechanism regulating the G1/S transition.
Tvegård T et al. Genes Dev 2007 Mar 15;21(6):649-54
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:23687372 - New roles of the fission yeast eIF2α kinases Hri1 and Gcn2 in response to nutritional stress.
Martín R et al. J Cell Sci 2013 Jul 15;126(Pt 14):3010-20
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7