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protein coding gene - nem1 (SPBC3B8.10c) - serine/threonine protein phosphatase (Nem1-Spo7 complex) catalytic subunit Nem1

Gene summary

Standard name
nem1
Systematic ID
SPBC3B8.10c
Product
serine/threonine protein phosphatase (Nem1-Spo7 complex) catalytic subunit Nem1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O59718
ORFeome ID
22/22A04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3373302..3375552 forward strand

Annotation

GO biological process

GO:0019915 - lipid storage

References:

GO:0071072 - negative regulation of phospholipid biosynthetic process

References:

GO:0071763 - nuclear membrane organization

References:

GO:0023052 - signaling

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GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0071595 - Nem1-Spo7 phosphatase complex

References:

GO:0005634 - nucleus

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GO molecular function

GO:0004722 - protein serine/threonine phosphatase activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0000356 - abnormal lipid droplet morphology

References:

Genotypes:

FYPO:0000769 - abnormal nuclear envelope morphology during vegetative growth

References:

Genotypes:

FYPO:0008376 - abolished protein localization to cell cortex of cell tip during cellular response to osmotic stress

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Genotypes:

FYPO:0001885 - decreased protein phosphorylation during salt stress

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0006664 - elongated nucleus during G0

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Genotypes:

FYPO:0006662 - fragmented nucleus during G0

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Genotypes:

FYPO:0006665 - increased nucleophagy during G0

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Genotypes:

FYPO:0006926 - increased nucleus:cytoplasm ratio

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Genotypes:

FYPO:0006791 - lipid droplets present in normal numbers

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Genotypes:

FYPO:0001007 - normal mitosis

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Genotypes:

FYPO:0003751 - normal nuclear envelope morphology

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Genotypes:

FYPO:0003780 - normal nuclear envelope morphology during mitosis

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Genotypes:

FYPO:0003781 - normal nuclear envelope morphology during mitotic interphase

References:

Genotypes:

FYPO:0001673 - normal nuclear morphology

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Genotypes:

FYPO:0006668 - normal nuclear morphology during G0

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Genotypes:

FYPO:0001380 - normal nuclear morphology during vegetative growth

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Genotypes:

FYPO:0001221 - normal nucleus:cytoplasm ratio

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Genotypes:

FYPO:0004467 - normal protein localization to cell tip during cellular response to salt stress

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000356 - abnormal lipid droplet morphology

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000769 - abnormal nuclear envelope morphology during vegetative growth

References:

Genotypes:

FYPO:0006667 - abnormal nuclear morphology during G0

References:

Genotypes:

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

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Genotypes:

FYPO:0003783 - abnormal nuclear pore localization during mitosis

References:

Genotypes:

FYPO:0007547 - abnormal nuclear pore localization to mitotic nuclear bridge midzone membrane

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0005584 - decreased cellular diglyceride level

References:

Genotypes:

FYPO:0005585 - decreased cellular triglyceride level during vegetative growth

References:

Genotypes:

FYPO:0006664 - elongated nucleus during G0

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

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Genotypes:

FYPO:0001556 - excess nuclear envelope present

References:

Genotypes:

FYPO:0007457 - excess nuclear envelope present during mitotic interphase

References:

Genotypes:

FYPO:0006662 - fragmented nucleus during G0

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0006345 - increased duration of protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0008171 - increased nuclear pore complex clustering

References:

Genotypes:

FYPO:0006665 - increased nucleophagy during G0

References:

Genotypes:

FYPO:0006926 - increased nucleus:cytoplasm ratio

References:

Genotypes:

FYPO:0002703 - increased protein level in autophagosome

References:

Genotypes:

FYPO:0007974 - increased rate of interpolar microtubule polymerization inside the mitotic nuclear membrane bridge

References:

Genotypes:

FYPO:0000238 - inviable cell upon G0 to G1 transition

References:

Genotypes:

FYPO:0002552 - lipid droplets present in decreased numbers

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

References:

Genotypes:

FYPO:0001673 - normal nuclear morphology

References:

Genotypes:

FYPO:0004467 - normal protein localization to cell tip during cellular response to salt stress

References:

Genotypes:

FYPO:0001266 - normal protein phosphorylation during cellular response to salt stress

References:

Genotypes:

FYPO:0004725 - nuclear envelope protrusion present during mitotic interphase

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000271 - sensitive to salt stress

References:

Genotypes:

FYPO:0005889 - sensitive to sodium chloride

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0114857 - there is a gene with the systematic identifier SPBC3B8.10 (ina17)

Protein features

IDNameInterPro nameDB name
PF03031NIFFCP1_domPfam
cd07521HAD_FCP1-likeCDD
PS50969FCP1FCP1_domPROSITE profiles
SM00577CPDcFCP1_domSMART
G3DSA:3.40.50.1000:FF:000093CATH-FunFam
G3DSA:3.40.50.1000HAD_sfCATH-Gene3D
SSF56784HAD-like_sfSUPERFAMILY
PTHR12210TIM50PANTHER
TIGR02251HIF-SF_eukDullard_phosphataseNCBIFAM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:36695178 - Diacylglycerol at the inner nuclear membrane fuels nuclear envelope expansion in closed mitosis.
Foo S et al. J Cell Sci 2023 Feb 01;136(3)
GO_REF:0000051 - S. pombe keyword mapping
PMID:35293864 - Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B.
Lera-Ramirez M et al. Elife 2022 Mar 16;11
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:31927482 - Splicing Defects of the Profilin Gene Alter Actin Dynamics in an S. pombe SMN Mutant.
Antoine M et al. iScience 2020 Jan 24;23(1):100809
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:30116786 - Genetic regulation of mitotic competence in G 0 quiescent cells.
Sajiki K et al. Sci Adv 2018 Aug;4(8):eaat5685
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:28545058 - A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control.
Kume K et al. PLoS Genet 2017 May;13(5):e1006767
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:33419777 - Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
Scotchman E et al. J Cell Sci 2021 Feb 11;134(3)
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:35354597 - Quantitative analysis of nuclear pore complex organization in Schizosaccharomyces pombe .
Varberg JM et al. Life Sci Alliance 2022 Jul;5(7)
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:39540318 - Pck2 association with the plasma membrane and efficient response of the cell integrity pathway require regulation of PI4P homeostasis by exomer.
Moscoso-Romero E et al. Open Biol 2024 Nov;14(11):240101
PMID:32848252 - Closed mitosis requires local disassembly of the nuclear envelope.
Dey G et al. Nature 2020 Sep;585(7823):119-123
PMID:31015410 - Nuclear membrane protein Lem2 regulates nuclear size through membrane flow.
Kume K et al. Nat Commun 2019 Apr 23;10(1):1871
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:36793083 - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes.
Mahrik L et al. Epigenetics Chromatin 2023 Feb 16;16(1):6
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105