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protein coding gene - rpa43 (SPBC3B9.07c) - DNA-directed RNA polymerase I complex subunit Rpa43

Gene summary

Standard name
rpa43
Systematic ID
SPBC3B9.07c
Product
DNA-directed RNA polymerase I complex subunit Rpa43
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
rpa21
UniProt ID
O43036
ORFeome ID
40/40A05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4000467..4001243 reverse strand

Annotation

PBO:0000445 - 2.7.7.6

GO biological process

GO:0042790 - nucleolar large rRNA transcription by RNA polymerase I

References:

GO:0006363 - termination of RNA polymerase I transcription

References:

GO:0006362 - transcription elongation by RNA polymerase I

References:

GO:0006361 - transcription initiation at RNA polymerase I promoter

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO:0005736 - RNA polymerase I complex

References:

GO molecular function

GO:0003899 - DNA-directed RNA polymerase activity

References:

Multi-locus phenotype

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000027 - ribosomal density decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003601 - decreased rRNA precursor level

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03876SHS2_Rpb7-NRpb7-like_NPfam
PF17875RPA43_OBRPA43_OBPfam
G3DSA:3.30.1490.120:FF:000004CATH-FunFam
G3DSA:2.40.50.1060CATH-Gene3D
G3DSA:3.30.1490.120RNA_pol_Rpb7-like_N_sfCATH-Gene3D
PTHR12709Rpb7-likePANTHER

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:12207036 - The fission yeast RPA21 subunit of RNA polymerase I: an evolutionarily conserved subunit interacting with ribosomal DNA (rDNA) transcription factor Rrn3p for recruitment to rDNA promoter.
Imazawa Y et al. Genes Genet Syst 2002 Jun;77(3):147-57
PMID:31294478 - Senataxin homologue Sen1 is required for efficient termination of RNA polymerase III transcription.
Rivosecchi J et al. EMBO J 2019 Aug 15;38(16):e101955
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:15620657 - Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast.
Wilkinson CR et al. Curr Biol 2004 Dec 29;14(24):2283-8
PMID:15647272 - The fission yeast protein Ker1p is an ortholog of RNA polymerase I subunit A14 in Saccharomyces cerevisiae and is required for stable association of Rrn3p and RPA21 in RNA polymerase I.
Imazawa Y et al. J Biol Chem 2005 Mar 25;280(12):11467-74
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:33536435 - Conserved strategies of RNA polymerase I hibernation and activation.
Heiss FB et al. Nat Commun 2021 Feb 03;12(1):758
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31