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protein coding gene - isa2 (SPBC3B9.17) - mitochondrial [4Fe-4S] cluster assembly and transfer protein Isa2

Gene summary

Standard name
isa2
Systematic ID
SPBC3B9.17
Product
mitochondrial [4Fe-4S] cluster assembly and transfer protein Isa2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O43045
ORFeome ID
08/08A01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4020272..4021107 forward strand

Annotation

Disease association

MONDO:0014611 - multiple mitochondrial dysfunctions syndrome 4

References:

GO biological process

GO:0044572 - [4Fe-4S] cluster assembly

References:

GO:0051604 - protein maturation

References:

GO cellular component

GO:0120510 - mitochondrial [4Fe-4S] assembly complex

References:

GO:0005739 - mitochondrion

References:

GO molecular function

GO:0051537 - 2 iron, 2 sulfur cluster binding

References:

GO:0051539 - 4 iron, 4 sulfur cluster binding

References:

GO:0005506 - iron ion binding

References:

GO:0005515 - protein binding

References:

Multi-locus phenotype

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PS01152HESBFeS_cluster_insertion_CSPROSITE patterns
G3DSA:2.60.300.12:FF:000006CATH-FunFam
G3DSA:2.60.300.12HesB-like_dom_sfCATH-Gene3D
SSF89360HesB-like_dom_sfSUPERFAMILY
PTHR43011PANTHER
TIGR00049ATAPNCBIFAM

Orthologs

References / Literature

PMID:20085751 - Monothiol glutaredoxin Grx5 interacts with Fe-S scaffold proteins Isa1 and Isa2 and supports Fe-S assembly and DNA integrity in mitochondria of fission yeast.
Kim KD et al. Biochem Biophys Res Commun 2010 Feb 12;392(3):467-72
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:34660592 - Biogenesis of Iron-Sulfur Clusters and Their Role in DNA Metabolism.
Shi R et al. Front Cell Dev Biol 2021;9:735678
PMID:34706246 - Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns.
Hümmer S et al. Cell Rep 2021 Oct 26;37(4):109893
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37156397 - Identification of novel coenzyme Q 10 biosynthetic proteins Coq11 and Coq12 in Schizosaccharomyces pombe.
Nishida I et al. J Biol Chem 2023 Jun;299(6):104797
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:25347204 - Formation of [4Fe-4S] clusters in the mitochondrial iron-sulfur cluster assembly machinery.
Brancaccio D et al. J Am Chem Soc 2014 Nov 19;136(46):16240-50
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000033 - Annotation inferences using phylogenetic trees