protein coding gene - trz2 (SPBC3D6.03c) - mitochondrial 3'-tRNA processing endonuclease tRNAse Z, Trz2

Gene summary

Standard name: trz2
Systematic ID: SPBC3D6.03c
Product: mitochondrial 3'-tRNA processing endonuclease tRNAse Z, Trz2
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: sptrz2, 3'-tRNase
UniProt ID: P87168
ORFeome ID: 27/27E12
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome II: 1271776..1274094 reverse strand

Annotation

Disease association

MONDO:0014190 - combined oxidative phosphorylation defect type 17

References:

PB_REF:0000006

MONDO:0013872 - prostate cancer, hereditary, 2

References:

PB_REF:0000006

GO biological process

GO:0140040 - mitochondrial polycistronic RNA processing

References:

PMID:23928301

GO:1990180 - mitochondrial tRNA 3'-end processing

References:

PMID:23928301

GO:0042780 - tRNA 3'-end processing

References:

PMID:21208191

GO cellular component

GO:0005737 - cytoplasm

References:

PMID:16823372

GO:0005759 - mitochondrial matrix

References:

GO_REF:0000024

GO:0005739 - mitochondrion

References:

PMID:21208191

GO molecular function

GO:0042781 - 3'-tRNA processing endoribonuclease activity

References:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

SO:0000418 - signal_peptide

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PMID:31626996

PomGeneEx:0000012 - RNA level decreased

References:

PMID:33313903

Quantitative gene expression

PBO:0006310 - protein level

References:

PMID:23101633

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000375 - abolished apoptotic process

References:

PMID:30393157

Genotypes:

trz2deltaMTS(1-38 Δaa)[Overexpression]

FYPO:0004481 - abolished cell population growth at high temperature

References:

PMID:33823662

Genotypes:

trz2-1(A623V aa)

FYPO:0000082 - decreased cell population growth at high temperature

References:

PMID:23928301

Genotypes:

trz2-1(A623V aa)

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

PMID:33823662

Genotypes:

trz2-1(A623V aa)

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

PMID:27902423

Genotypes:

trz2(promoter-delta-HomolD)((-35)-(-27))

FYPO:0002584 - decreased mature mitochondrial tRNA level

References:

Genotypes:

trz2-1(A623V aa)

FYPO:0004944 - decreased mitochondrial membrane potential

References:

PMID:30393157

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0007610 - decreased mitochondrial volume

References:

PMID:33823662

Genotypes:

trz2-1(A623V aa)

FYPO:0000781 - decreased transcription during vegetative growth

References:

PMID:27902423

Genotypes:

trz2(promoter-110--54)((-110)-(-54))

FYPO:0001355 - decreased vegetative cell population growth

References:

PMID:23928301

Genotypes:

FYPO:0006661 - fragmented nucleus

References:

PMID:30393157

Genotypes:

FYPO:0002006 - increased cellular iron level

References:

PMID:30393157

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0003004 - increased cellular reactive oxygen species level during vegetative growth

References:

PMID:30393157

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0000377 - increased frequency of apoptosis

References:

PMID:30393157

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0006783 - increased level of carbohydrate metabolism gene mRNA during vegetative growth

References:

PMID:30393157

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0005420 - increased level of iron assimilation gene mRNA during vegetative growth

References:

PMID:30393157

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0002587 - increased level of mitochondrial polycistronic RNA precursors

References:

PMID:23928301

Genotypes:

trz2-1(A623V aa)

FYPO:0002590 - increased level of mitochondrial tRNA processing intermediates with 3' extensions

References:

Genotypes:

trz2-1(A623V aa)

FYPO:0007737 - increased mitochondrial membrane potential

References:

PMID:33823662

Genotypes:

trz2-1(A623V aa)

FYPO:0006446 - increased mitochondrial pre-mRNA level

References:

PMID:29954949

Genotypes:

trz2-1(A623V aa)

FYPO:0005823 - increased phosphatidylserine externalization

References:

PMID:30393157

Genotypes:

FYPO:0000780 - increased transcription during vegetative growth

References:

PMID:27902423

Genotypes:

trz2(promoter-110--54)((-110)-(-54))

FYPO:0002111 - inviable tapered vegetative cell

References:

PMID:23697806

Genotypes:

trz2delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002199 - inviable vegetative cell with normal cell morphology

References:

PMID:33823662

Genotypes:

trz2-1(A623V aa)

FYPO:0000224 - lemon-shaped cell

References:

PMID:21208191

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0006660 - loss of viability upon G0 to G1 transition

References:

PMID:33823662

Genotypes:

trz2-1(A623V aa)

FYPO:0002585 - mature mitochondrial tRNA absent from cell

References:

PMID:23928301

Genotypes:

trz2-1(A623V aa)

FYPO:0002580 - normal mature tRNA level

References:

PMID:23928301

Genotypes:

trz2-1(A623V aa)

FYPO:0003896 - normal mitochondrial morphology

References:

PMID:33823662

Genotypes:

trz2-1(A623V aa)

FYPO:0001673 - normal nuclear morphology

References:

PMID:30393157

Genotypes:

trz2deltaMTS(1-38 Δaa)[Overexpression]

FYPO:0001855 - normal transcription during vegetative growth

References:

PMID:27902423

Genotypes:

trz2(promoter--110--54,G-102C,T-99A)((-110)-(-54),G(-102)C,T(-99)A)

FYPO:0000783 - protein mislocalized to cytoplasm during vegetative growth

References:

PMID:21208191

Genotypes:

trz2deltaMTS(1-38 Δaa)

FYPO:0003810 - small fragmented mitochondria present in increased numbers

References:

PMID:30393157

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0000021 - spheroid vegetative cell

References:

PMID:21208191

Genotypes:

trz2+(wild type)[Overexpression]

FYPO:0002060 - viable vegetative cell population

References:

PMID:30393157

Genotypes:

trz2+(wild type)[Overexpression]

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF00753	Lactamase_B	Metallo-B-lactamas	Pfam
PF13691	Lactamase_B_4	tRNase_Z_dom	Pfam
cd07718	RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold		CDD
SM00849	Lactamase_B	Metallo-B-lactamas	SMART
G3DSA:3.60.15.10:FF:000157			CATH-FunFam
G3DSA:3.60.15.10		RibonucZ/Hydroxyglut_hydro	CATH-Gene3D
SSF56281		RibonucZ/Hydroxyglut_hydro	SUPERFAMILY
PTHR12553		RNZ2-like	PANTHER

Orthologs

ELAC1 (HGNC:14197) Homo sapiens
ELAC2 (HGNC:14198) Homo sapiens
trz2 (SJAG_04885) Schizosaccharomyces japonicus
TRZ1 (YKR079C) Saccharomyces cerevisiae

References / Literature

PMID:33823662 - Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins.: Uehara L et al. Open Biol 2021 Apr;11(4):200369
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21208191 - The fission yeast Schizosaccharomyces pombe has two distinct tRNase Z(L)s encoded by two different genes and differentially targeted to the nucleus and mitochondria.: Gan X et al. Biochem J 2011 Apr 01;435(1):103-11
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.: Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.: Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:27902423 - Characterization of cis -elements in the promoter of trz2 encoding Schizosaccharomyces pombe mitochondrial tRNA 3'-end processing enzyme.: Liu J et al. Microbiology (Reading) 2017 Jan;163(1):75-85
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.: Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:23928301 - Partitioning of the nuclear and mitochondrial tRNA 3'-end processing activities between two different proteins in Schizosaccharomyces pombe.: Zhang X et al. J Biol Chem 2013 Sep 20;288(38):27415-27422
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:29954949 - The S. pombe mitochondrial transcriptome.: Shang J et al. RNA 2018 Sep;24(9):1241-1254
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30393157 - Overexpression of Schizosaccharomyces pombe tRNA 3'-end processing enzyme Trz2 leads to an increased cellular iron level and apoptotic cell death.: Shang J et al. Fungal Genet Biol 2019 Jan;122:11-20
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.: UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;