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protein coding gene - dpb4 (SPBC3D6.09) - DNA polymerase epsilon subunit Dpb4

Gene summary

Standard name
dpb4
Systematic ID
SPBC3D6.09
Product
DNA polymerase epsilon subunit Dpb4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P87174
ORFeome ID
10/10B08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1285946..1287121 forward strand

Annotation

GO biological process

GO:0140529 - CMG complex assembly

References:

GO:0006974 - DNA damage response

References:

GO:0006335 - DNA replication-dependent chromatin assembly

References:

GO:1902983 - DNA strand elongation involved in mitotic DNA replication

References:

GO:0040029 - epigenetic regulation of gene expression

References:

GO:0031507 - heterochromatin formation

References:

GO:0006272 - leading strand elongation

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GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0008622 - epsilon DNA polymerase complex

References:

GO:0043596 - nuclear replication fork

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0031490 - chromatin DNA binding

References:

GO:0031491 - nucleosome binding

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0007478 - decreased epigenetic heterochromatin inheritance

References:

Genotypes:

FYPO:0008185 - decreased histone H3-K9 trimethylation at centromere central core during vegetative growth

References:

Genotypes:

FYPO:0000890 - decreased histone H3-K9 trimethylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0008186 - normal histone H3-K9 trimethylation at centromere central core during vegetative growth

References:

Genotypes:

Protein features

PBO:0111763 - histone fold

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0007376 - abolished epigenetic heterochromatin inheritance

References:

Genotypes:

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0007478 - decreased epigenetic heterochromatin inheritance

References:

Genotypes:

FYPO:0000890 - decreased histone H3-K9 trimethylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0002386 - decreased protein localization to pericentric heterochromatin at centromere outer repeat region

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0008186 - normal histone H3-K9 trimethylation at centromere central core during vegetative growth

References:

Genotypes:

FYPO:0008199 - normal histone H3-K9 trimethylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0009039 - resistance to potassium chloride

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00808CBFD_NFYB_HMFCBFA_NFYB_domainPFAM
cd22928HFD_POLE3_DPB4CDD
SSF47113Histone-foldHistone-foldSUPERFAMILY
G3DSA:1.10.20.10Histone, subunit AHistone-foldGENE3D
PTHR46172DNA POLYMERASE EPSILON SUBUNIT 3DNA_Pol-Epsilon_SubunitPANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMOBIDB-Negative-Polyelectrolyte
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:29109278 - Coordinated regulation of heterochromatin inheritance by Dpb3-Dpb4 complex.
He H et al. Proc Natl Acad Sci U S A 2017 Nov 21;114(47):12524-12529
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:38479839 - Coordination of histone chaperones for parental histone segregation and epigenetic inheritance.
Fang Y et al. Genes Dev 2024 Mar 13;
PMID:40861011 - Faithful inheritance: Parental histone recycling and epigenetic memory in fission yeast.
Fang Y et al. Cell Insight 2025 Oct;4(5):100275
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:33693625 - Dri1 mediates heterochromatin assembly via RNAi and histone deacetylation.
Ban H et al. Genetics 2021 May 17;218(1)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:15388803 - Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast.
Spiga MG et al. Nucleic Acids Res 2004;32(16):4945-53
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:19109429 - GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.
Pai CC et al. Mol Biol Cell 2009 Feb;20(4):1213-22
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257