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protein coding gene - slx8 (SPBC3D6.11c) - SUMO-targeted ubiquitin-protein ligase E3 Slx8

Gene summary

Standard name
slx8
Systematic ID
SPBC3D6.11c
Product
SUMO-targeted ubiquitin-protein ligase E3 Slx8
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P87176
ORFeome ID
10/10D01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1288188..1290211 reverse strand

Annotation

Complementation

PBO:0001118 - functionally complemented by human RNF4

References:

GO biological process

GO:0072766 - centromere clustering at the mitotic interphase nuclear envelope

References:

GO:0006310 - DNA recombination

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GO:0120290 - stalled replication fork localization to nuclear periphery

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GO:0006511 - ubiquitin-dependent protein catabolic process

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GO cellular component

GO:0000785 - chromatin

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GO:0000775 - chromosome, centromeric region

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GO:0031934 - mating-type region heterochromatin

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GO:0044732 - mitotic spindle pole body

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GO:0034399 - nuclear periphery

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GO:0005634 - nucleus

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GO:0033768 - SUMO-targeted ubiquitin ligase complex

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GO:0000781 - chromosome, telomeric region

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GO molecular function

GO:0003677 - DNA binding

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GO:0032183 - SUMO binding

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GO:0061630 - ubiquitin protein ligase activity

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GO:0004842 - ubiquitin-protein transferase activity

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Modification

MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0005630 - decreased cellular HMW SUMO conjugate level

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Genotypes:

FYPO:0006800 - decreased centromere clustering at nuclear periphery during mitotic interphase

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Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0005629 - increased cellular HMW SUMO conjugate level

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Genotypes:

FYPO:0005619 - increased level of sumoylated protein in cell

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Genotypes:

FYPO:0005620 - increased SUMO chain length

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Genotypes:

FYPO:0005621 - increased topoisomerase-DNA adduct level

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Genotypes:

FYPO:0001690 - normal growth on camptothecin

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Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

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Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

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Genotypes:

FYPO:0002776 - normal level of sumoylated protein in cell

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0006320 - normal replication slippage during replication fork processing

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

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Genotypes:

FYPO:0002176 - viable vegetative cell with normal cell size

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Genotypes:

Protein features

PBO:0111835 - human RNF4 family

PBO:0111749 - ubiquitin-protein ligase E3

PBO:0111746 - zf-C3HC4 type (RING finger)

PBO:0111743 - zinc finger protein

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0005630 - decreased cellular HMW SUMO conjugate level

References:

Genotypes:

FYPO:0006800 - decreased centromere clustering at nuclear periphery during mitotic interphase

References:

Genotypes:

FYPO:0003411 - decreased chromatin silencing at centromere inner repeat

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Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0000658 - decreased DNA binding

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Genotypes:

FYPO:0002768 - decreased protein ubiquitination during vegetative growth

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Genotypes:

FYPO:0007532 - decreased replication fork colocalization with nuclear pore complex

References:

Genotypes:

FYPO:0003589 - decreased replication slippage during replication fork processing

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005629 - increased cellular HMW SUMO conjugate level

References:

Genotypes:

FYPO:0005619 - increased level of sumoylated protein in cell

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0005620 - increased SUMO chain length

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Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001690 - normal growth on camptothecin

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0002776 - normal level of sumoylated protein in cell

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0001668 - normal protein processing during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

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Genotypes:

FYPO:0007533 - normal replication fork colocalization with nuclear pore complex

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0005750 - sensitive to Zeocin

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF13920zf-C3HC4_3Pfam
PS00518ZF_RING_1Znf_RING_CSPROSITE patterns
PS50089ZF_RING_2Znf_RINGPROSITE profiles
SM00184RINGZnf_RINGSMART
G3DSA:3.30.40.10Znf_RING/FYVE/PHDCATH-Gene3D
SSF57850SUPERFAMILY
PTHR47094SLX8PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:24265825 - Concerted action of the ubiquitin-fusion degradation protein 1 (Ufd1) and Sumo-targeted ubiquitin ligases (STUbLs) in the DNA-damage response.
Køhler JB et al. PLoS One 2013;8(11):e80442
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:41330900 - PolySUMOylation of PCNA and Rad52 restricts centromeric recombination in fission yeast.
Markowska K et al. Nat Commun 2025 Dec 02;16(1):10837
PMID:33159083 - The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal.
Kramarz K et al. Nat Commun 2020 Nov 06;11(1):5643
PMID:39786922 - The fission yeast SUMO-targeted ubiquitin ligase Slx8 functionally associates with clustered centromeres and the silent mating-type region at the nuclear periphery.
Chakraborty S et al. Biol Open 2024 Dec 15;13(12)
PMID:26221037 - Pli1(PIAS1) SUMO ligase protected by the nuclear pore-associated SUMO protease Ulp1SENP1/2.
Nie M et al. J Biol Chem 2015 Sep 11;290(37):22678-85
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:28475613 - SUMO-targeted ubiquitin ligase activity can either suppress or promote genome instability, depending on the nature of the DNA lesion.
Nie M et al. PLoS Genet 2017 May;13(5):e1006776
PMID:28552615 - SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage.
Wei Y et al. Mol Cell 2017 Jun 01;66(5):581-596.e6
PMID:27398807 - Functional Crosstalk between the PP2A and SUMO Pathways Revealed by Analysis of STUbL Suppressor, razor 1-1.
Nie M et al. PLoS Genet 2016 Jul;12(7):e1006165
PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9
PMID:21408210 - SUMO-targeted ubiquitin ligase, Rad60, and Nse2 SUMO ligase suppress spontaneous Top1-mediated DNA damage and genome instability.
Heideker J et al. PLoS Genet 2011 Mar;7(3):e1001320
PMID:17762864 - Conserved function of RNF4 family proteins in eukaryotes: targeting a ubiquitin ligase to SUMOylated proteins.
Sun H et al. EMBO J 2007 Sep 19;26(18):4102-12
PMID:21444718 - DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes.
Prudden J et al. Mol Cell Biol 2011 Jun;31(11):2299-310
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23936535 - Slx8 removes Pli1-dependent protein-SUMO conjugates including SUMOylated topoisomerase I to promote genome stability.
Steinacher R et al. PLoS One 2013;8(8):e71960
PMID:17762865 - SUMO-targeted ubiquitin ligases in genome stability.
Prudden J et al. EMBO J 2007 Sep 19;26(18):4089-101
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:19363481 - Molecular mimicry of SUMO promotes DNA repair.
Prudden J et al. Nat Struct Mol Biol 2009 May;16(5):509-16