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protein coding gene - hsp16 (SPBC3E7.02c) - heat shock protein Hsp16

Gene summary

Standard name
hsp16
Systematic ID
SPBC3E7.02c
Product
heat shock protein Hsp16
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14368
ORFeome ID
31/31D08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2660139..2660992 reverse strand

Annotation

Disease association

MONDO:0013411 - cataract 16 multiple types

References:

MONDO:0011413 - cataract 9 multiple types

References:

MONDO:0015626 - Charcot-Marie-Tooth disease

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MONDO:0011687 - Charcot-Marie-Tooth disease axonal type 2F

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MONDO:0012096 - Charcot-Marie-Tooth disease axonal type 2L

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MONDO:0014073 - dilated cardiomyopathy 1II

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MONDO:0013472 - fatal infantile hypertonic myofibrillar myopathy

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MONDO:0012130 - myofibrillar myopathy 2

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MONDO:0976133 - myopathy, myofibrillar, 13, with rimmed vacuoles

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MONDO:0005071 - nervous system disorder

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MONDO:0008025 - neuronopathy, distal hereditary motor, type 2A

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MONDO:0012080 - neuronopathy, distal hereditary motor, type 2B

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MONDO:0013243 - neuronopathy, distal hereditary motor, type 2C

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GO biological process

GO:0034605 - cellular response to heat

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GO:0071218 - cellular response to misfolded protein

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GO:0034620 - cellular response to unfolded protein

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GO:0006457 - protein folding

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GO:0009651 - response to salt stress

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO:0140453 - protein aggregate center

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GO molecular function

GO:0005515 - protein binding

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GO:0140309 - unfolded protein holdase activity

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Miscellaneous functional group

PBO:0000255 - chaperone

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002522 - decreased poly(A)+ mRNA export from nucleus

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Genotypes:

FYPO:0004179 - decreased protein aggregate fusion

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0007301 - normal protein aggregate center formation

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

References:

PBO:0017093 - homomeric(6)

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Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00011HSP20A-crystallin/Hsp20_domPFAM
cd06464ACD_sHsps-likeCDD
PS01031SHSPA-crystallin/Hsp20_domPROSITE_PROFILES
G3DSA:2.60.40.790:FF:000072FUNFAM
SSF49764HSP20-like chaperonesHSP20-like_chaperoneSUPERFAMILY
G3DSA:2.60.40.790HSP20-like_chaperoneGENE3D
PTHR11527HEAT-SHOCK PROTEIN 20 FAMILY MEMBERSmall_HSPPANTHER

Orthologs

References / Literature

PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:11452028 - Expression of hsp16 in response to nucleotide depletion is regulated via the spc1 MAPK pathway in Schizosaccharomyces pombe.
Taricani L et al. Nucleic Acids Res 2001 Jul 15;29(14):3030-40
PMID:12482946 - Dicer is required for chromosome segregation and gene silencing in fission yeast cells.
Provost P et al. Proc Natl Acad Sci U S A 2002 Dec 24;99(26):16648-53
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:16428807 - Hip3 interacts with the HIRA proteins Hip1 and Slm9 and is required for transcriptional silencing and accurate chromosome segregation.
Greenall A et al. J Biol Chem 2006 Mar 31;281(13):8732-9
PMID:16055437 - Interaction of a small heat shock protein of the fission yeast, Schizosaccharomyces pombe, with a denatured protein at elevated temperature.
Hirose M et al. J Biol Chem 2005 Sep 23;280(38):32586-93
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:15947136 - RNA polymerase II is required for RNAi-dependent heterochromatin assembly.
Kato H et al. Science 2005 Jul 15;309(5733):467-9
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:17614284 - The chromatin-remodeling factor FACT contributes to centromeric heterochromatin independently of RNAi.
Lejeune E et al. Curr Biol 2007 Jul 17;17(14):1219-24
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:21828039 - Mfc1 is a novel forespore membrane copper transporter in meiotic and sporulating cells.
Beaudoin J et al. J Biol Chem 2011 Sep 30;286(39):34356-72
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:27966061 - UBL/BAG-domain co-chaperones cause cellular stress upon overexpression through constitutive activation of Hsf1.
Poulsen EG et al. Cell Stress Chaperones 2017 Jan;22(1):143-154
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.
Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.
Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:33946513 - The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.
Yukawa M et al. Int J Mol Sci 2021 Apr 30;22(9)
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:23273429 - Nonequivalence observed for the 16-meric structure of a small heat shock protein, SpHsp16.0, from Schizosaccharomyces pombe.
Hanazono Y et al. Structure 2013 Feb 05;21(2):220-8
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24936793 - Fusion of protein aggregates facilitates asymmetric damage segregation.
Coelho M et al. PLoS Biol 2014 Jun;12(6):e1001886
PMID:23293578 - Molecular genetics of charcot-marie-tooth disease: from genes to genomes.
Azzedine H et al. Mol Syndromol 2012 Nov;3(5):204-14
PMID:32075773 - Chaperone-Facilitated Aggregation of Thermo-Sensitive Proteins Shields Them from Degradation during Heat Stress.
Cabrera M et al. Cell Rep 2020 Feb 18;30(7):2430-2443.e4
PMID:33225241 - The Hsp40 Mas5 Connects Protein Quality Control and the General Stress Response through the Thermo-sensitive Pyp1.
Boronat S et al. iScience 2020 Nov 20;23(11):101725
PMID:40063661 - Fission yeast Caprin protein is required for efficient heterochromatin establishment.
Zhang H et al. PLoS Genet 2025 Mar 10;21(3):e1011620
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:41259369 - Hydroxyurea induces an oxidative stress response that triggers ER expansion and cytoplasmic protein aggregation.
Sánchez-Molina A et al. PLoS Biol 2025 Nov 19;23(11):e3003493
PMID:33386485 - Spatial sequestration of misfolded proteins as an active chaperone-mediated process during heat stress.
Boronat S et al. Curr Genet 2021 Apr;67(2):237-243
PMID:15840944 - Hsp16p is required for thermotolerance in nuclear mRNA export in fission yeast Schizosaccharomyces pombe.
Yoshida J et al. Cell Struct Funct 2005 Feb;29(5-6):125-38
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:34460892 - Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis.
Tarhan Ç et al. Genet Mol Biol 2021;44(3):e20200245
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:36174923 - Schizosaccharomyces pombe MAP kinase Sty1 promotes survival of Δppr10 cells with defective mitochondrial protein synthesis.
Hu Y et al. Int J Biochem Cell Biol 2022 Nov;152:106308
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;