protein coding gene - rad13 (SPBC3E7.08c) - DNA repair nuclease Rad13

Gene summary

Standard name

rad13

Systematic ID

SPBC3E7.08c

Product

DNA repair nuclease Rad13

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

P28706

ORFeome ID

34/34A07

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 2672990..2676569 reverse strand

Annotation

Protein features

Orthologs

• ERCC5 (HGNC:3437) Homo sapiens
• rad13 (SJAG_02031) Schizosaccharomyces japonicus
• RAD2 (YGR258C) Saccharomyces cerevisiae

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:15367656 - Swi1 and Swi3 are components of a replication fork protection complex in fission yeast.

Noguchi E et al. Mol Cell Biol 2004 Oct;24(19):8342-55

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:9563836 - Mutant PCNA alleles are associated with cdc phenotypes and sensitivity to DNA damage in fission yeast.

Arroyo MP et al. Mol Gen Genet 1998 Mar;257(5):505-18

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:9372918 - Role of Schizosaccharomyces pombe RecQ homolog, recombination, and checkpoint genes in UV damage tolerance.

Murray JM et al. Mol Cell Biol 1997 Dec;17(12):6868-75

PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.

Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:8846774 - Fission yeast rad17: a homologue of budding yeast RAD24 that shares regions of sequence similarity with DNA polymerase accessory proteins.

Griffiths DJ et al. EMBO J 1995 Dec 01;14(23):5812-23

PMID:12000964 - Cnd2 has dual roles in mitotic condensation and interphase.

Aono N et al. Nature 2002 May 09;417(6885):197-202

PMID:21493688 - Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation.

Guarino E et al. Nucleic Acids Res 2011 Aug;39(14):5978-90

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:10446227 - Characterization of the rhp7(+) and rhp16(+) genes in Schizosaccharomyces pombe.

Lombaerts M et al. Nucleic Acids Res 1999 Sep 01;27(17):3410-6

PMID:18675827 - The tumor suppressor homolog in fission yeast, myh1(+), displays a strong interaction with the checkpoint gene rad1(+).

Jansson K et al. Mutat Res 2008 Sep 26;644(1-2):48-55

PMID:16912194 - Cross-talk between nucleotide excision and homologous recombination DNA repair pathways in the mechanism of action of antitumor trabectedin.

Herrero AB et al. Cancer Res 2006 Aug 15;66(16):8155-62

PMID:22665798 - Induction of a G1-S checkpoint in fission yeast.

Bøe CA et al. Proc Natl Acad Sci U S A 2012 Jun 19;109(25):9911-6

PMID:41525994 - Transcriptional PBR cycles at pericentromeric repeats cause gross chromosomal rearrangements through Rad52-dependent ADR-loop formation.

Xu R et al. Nucleic Acids Res 2026 Jan 05;54(1)

PMID:21183410 - Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.

Vejrup-Hansen R et al. DNA Repair (Amst) 2011 Mar 07;10(3):283-95

PMID:15329725 - Separase-mediated cleavage of cohesin at interphase is required for DNA repair.

Nagao K et al. Nature 2004 Aug 26;430(7003):1044-8

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:7254221 - Spontaneous and UV-induced recombination in radiation-sensitive mutants of Schizosaccharomyces pombe.

Grossenbacher-Grunder AM et al. Mutat Res 1981 Mar;81(1):37-48

PMID:22064477 - The RecQ4 orthologue Hrq1 is critical for DNA interstrand cross-link repair and genome stability in fission yeast.

Groocock LM et al. Mol Cell Biol 2012 Jan;32(2):276-87

PMID:14765108 - Recovery from DNA damage checkpoint arrest by PP1-mediated inhibition of Chk1.

den Elzen NR et al. EMBO J 2004 Feb 25;23(4):908-18

PMID:8464724 - Evolutionary conservation of excision repair in Schizosaccharomyces pombe: evidence for a family of sequences related to the Saccharomyces cerevisiae RAD2 gene.

Carr AM et al. Nucleic Acids Res 1993 Mar 25;21(6):1345-9

PMID:18344406 - Schizosaccharomyces pombe Hst4 functions in DNA damage response by regulating histone H3 K56 acetylation.

Haldar D et al. Eukaryot Cell 2008 May;7(5):800-13

PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.

Vega M et al. BMC Biol 2022 Jul 12;20(1):160

PMID:9153313 - Isolation and characterization of the Schizosaccharomyces pombe rhp9 gene: a gene required for the DNA damage checkpoint but not the replication checkpoint.

Willson J et al. Nucleic Acids Res 1997 Jun 01;25(11):2138-46

PMID:9870697 - Differential effects of caffeine on DNA damage and replication cell cycle checkpoints in the fission yeast Schizosaccharomyces pombe.

Osman F et al. Mol Gen Genet 1998 Nov;260(4):319-34

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:11523791 - A homologue of the Rad18 postreplication repair gene is required for DNA damage responses throughout the fission yeast cell cycle.

Verkade HM et al. Mol Genet Genomics 2001 Aug;265(6):993-1003

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:9092661 - Characterization of the alternative excision repair pathway of UV-damaged DNA in Schizosaccharomyces pombe.

Yonemasu R et al. Nucleic Acids Res 1997 Apr 15;25(8):1553-8

PMID:9613582 - Phosphoinositide-specific phospholipase C forms a complex with 14-3-3 proteins and is involved in expression of UV resistance in fission yeast.

Andoh T et al. Mol Gen Genet 1998 Apr;258(1-2):139-47

PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.

Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9

PMID:23071723 - DNA polymerase α (swi7) and the flap endonuclease Fen1 (rad2) act together in the S-phase alkylation damage response in S. pombe.

Koulintchenko M et al. PLoS One 2012;7(10):e47091

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:17151242 - Sap1 promotes the association of the replication fork protection complex with chromatin and is involved in the replication checkpoint in Schizosaccharomyces pombe.

Noguchi C et al. Genetics 2007 Feb;175(2):553-66

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:7623848 - An alternative eukaryotic DNA excision repair pathway.

Freyer GA et al. Mol Cell Biol 1995 Aug;15(8):4572-7

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:12181326 - Characterization of a Schizosaccharomyces pombe strain deleted for a sequence homologue of the human damaged DNA binding 1 (DDB1) gene.

Zolezzi F et al. J Biol Chem 2002 Oct 25;277(43):41183-91

PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.

Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.

Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8

PMID:12675805 - A new Schizosaccharomyces pombe base excision repair mutant, nth1, reveals overlapping pathways for repair of DNA base damage.

Osman F et al. Mol Microbiol 2003 Apr;48(2):465-80

PMID:10390527 - Removal of cyclobutane pyrimidine dimers by the UV damage repair and nucleotide excision repair pathways of Schizosaccharomyces pombe at nucleotide resolution.

Lombaerts M et al. Nucleic Acids Res 1999 Jul 15;27(14):2868-74

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:9023111 - The fission yeast UVDR DNA repair pathway is inducible.

Davey S et al. Nucleic Acids Res 1997 Mar 01;25(5):1002-8

PMID:11719193 - Mus81-Eme1 are essential components of a Holliday junction resolvase.

Boddy MN et al. Cell 2001 Nov 16;107(4):537-48

PB_REF:0000003 - Disease Association Curation

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:166019 - Genetic control of radiation sensitivity in Schizosaccharomyces pombe.

Nasim A et al. Genetics 1975 Apr;79(4):573-82

PMID:11800554 - Role of the DNA repair nucleases Rad13, Rad2 and Uve1 of Schizosaccharomyces pombe in mismatch correction.

Kunz C et al. J Mol Biol 2001 Oct 19;313(2):241-53

PMID:18231579 - The CDK-activating kinase (CAK) Csk1 is required for normal levels of homologous recombination and resistance to DNA damage in fission yeast.

Gerber HB et al. PLoS One 2008 Jan 30;3(1):e1492

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:19516334 - Flipping of alkylated DNA damage bridges base and nucleotide excision repair.

Tubbs JL et al. Nature 2009 Jun 11;459(7248):808-13

PMID:20885790 - Critical functions of Rpa3/Ssb3 in S-phase DNA damage responses in fission yeast.

Cavero S et al. PLoS Genet 2010 Sep 23;6(9):e1001138

PMID:11073977 - Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1.

Boddy MN et al. Mol Cell Biol 2000 Dec;20(23):8758-66

PMID:8290359 - Cloning and characterisation of the Schizosaccharomyces pombe rad8 gene, a member of the SNF2 helicase family.

Doe CL et al. Nucleic Acids Res 1993 Dec 25;21(25):5964-71

PMID:8524274 - The rad18 gene of Schizosaccharomyces pombe defines a new subgroup of the SMC superfamily involved in DNA repair.

Lehmann AR et al. Mol Cell Biol 1995 Dec;15(12):7067-80

PMID:8709952 - The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeast Schizosaccharomyces pombe.

Hegde V et al. Mol Gen Genet 1996 Jun 24;251(4):483-92

PMID:17307401 - The novel gene mus7(+) is involved in the repair of replication-associated DNA damage in fission yeast.

Yokoyama M et al. DNA Repair (Amst) 2007 Jun 01;6(6):770-80

PMID:9092625 - Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance.

Shayeghi M et al. Nucleic Acids Res 1997 Mar 15;25(6):1162-9

PMID:10735851 - Contribution of base excision repair, nucleotide excision repair, and DNA recombination to alkylation resistance of the fission yeast Schizosaccharomyces pombe.

Memisoglu A et al. J Bacteriol 2000 Apr;182(8):2104-12

PMID:31563844 - The roles of fission yeast exonuclease 5 in nuclear and mitochondrial genome stability.

Sparks JL et al. DNA Repair (Amst) 2019 Nov;83:102720

PMID:16478984 - The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.

Pebernard S et al. Mol Cell Biol 2006 Mar;26(5):1617-30

PMID:22645654 - Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.

Akai Y et al. Open Biol 2011 Dec;1(4):110023

PMID:9487130 - Regulation of telomere length by checkpoint genes in Schizosaccharomyces pombe.

Dahlen M et al. Mol Biol Cell 1998 Mar;9(3):611-21

PMID:10652237 - Identification and characterization of the rhp23(+) DNA repair gene in Schizosaccharomyces pombe.

Lombaerts M et al. Biochem Biophys Res Commun 2000 Feb 05;268(1):210-5

PMID:18769921 - Genetic analysis reveals different roles of Schizosaccharomyces pombe sfr1/dds20 in meiotic and mitotic DNA recombination and repair.

Khasanov FK et al. Curr Genet 2008 Oct;54(4):197-211

PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.

Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595

PMID:9658208 - Sensitivity to cisplatin and platinum-containing compounds of Schizosaccharomyces pombe rad mutants.

Perego P et al. Mol Pharmacol 1998 Jul;54(1):213-9

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:7885834 - Cloning and characterisation of the Schizosaccharomyces pombe rad32 gene: a gene required for repair of double strand breaks and recombination.

Tavassoli M et al. Nucleic Acids Res 1995 Feb 11;23(3):383-8

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:12023299 - Cdc2-cyclin B kinase activity links Crb2 and Rqh1-topoisomerase III.

Caspari T et al. Genes Dev 2002 May 15;16(10):1195-208

PMID:15485909 - Rad62 protein functionally and physically associates with the smc5/smc6 protein complex and is required for chromosome integrity and recombination repair in fission yeast.

Morikawa H et al. Mol Cell Biol 2004 Nov;24(21):9401-13

PMID:11452021 - Involvement of Schizosaccharomyces pombe Srs2 in cellular responses to DNA damage.

Wang SW et al. Nucleic Acids Res 2001 Jul 15;29(14):2963-72

PMID:10480889 - Transcription dependence and the roles of two excision repair pathways for UV damage in fission yeast Schizosaccharomyces pombe.

Yasuhira S et al. J Biol Chem 1999 Sep 17;274(38):26822-7

PMID:8879272 - Cloning of Schizosaccharomyces pombe rph16+, a gene homologous to the Saccharomyces cerevisiae RAD16 gene.

Bang DD et al. Mutat Res 1996 Oct 18;364(2):57-71

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36