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protein coding gene - trr1 (SPBC3F6.03) - thioredoxin reductase Trr1

Gene summary

Standard name
trr1
Systematic ID
SPBC3F6.03
Product
thioredoxin reductase Trr1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
caf4, trxR
UniProt ID
Q92375
ORFeome ID
41/41H04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4189799..4191448 forward strand

Annotation

PBO:0004849 - 1.8.1.9

GO biological process

GO:0045454 - cell redox homeostasis

References:

GO:0019430 - removal of superoxide radicals

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005739 - mitochondrion

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0004791 - thioredoxin-disulfide reductase (NADPH) activity

References:

Modification

MOD:00689 - disulfide crosslinked residues

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0003238 - decreased anaerobic cell population growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0004007 - decreased protein level during cellular response to menadione

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005048 - increased protein localization to chromatin at RNA polymerase II promoter during vegetative growth

References:

Genotypes:

FYPO:0001130 - increased protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0000219 - increased protein oxidation during vegetative growth

References:

Genotypes:

FYPO:0001571 - increased protein-protein interaction

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0004036 - increased thioredoxin-disulfide reductase activity

References:

Genotypes:

FYPO:0001753 - normal anaerobic cell population growth

References:

Genotypes:

FYPO:0001517 - resistance to aluminium

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0002312 - resistance to ionizing radiation during vegetative growth

References:

Genotypes:

FYPO:0001516 - resistance to mercury

References:

Genotypes:

FYPO:0001987 - sensitive to high pH

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF07992Pyr_redox_2FAD/NAD-binding_domPFAM
PS00573PYRIDINE_REDOX_2Pyr_nucl-diS_OxRdtase_2_ASPROSITE_PATTERNS
PR00368FADPNRPRINTS
PR00469PNDRDTASEIIPRINTS
G3DSA:3.50.50.60:FF:000064FUNFAM
SSF51905FAD/NAD(P)-binding domainFAD/NAD-bd_sfSUPERFAMILY
G3DSA:3.50.50.60FAD/NAD-bd_sfGENE3D
PTHR48105THIOREDOXIN REDUCTASE 1-RELATED-RELATEDFerredoxin-NADP_redctase_2PANTHER
TIGR01292TRX_reductThioredox_RdtaseNCBIFAM

Orthologs

References / Literature

PMID:24118096 - Methionine sulphoxide reductases revisited: free methionine as a primary target of H₂O₂stress in auxotrophic fission yeast.
García-Santamarina S et al. Mol Microbiol 2013 Dec;90(5):1113-24
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:17409354 - The peroxiredoxin Tpx1 is essential as a H2O2 scavenger during aerobic growth in fission yeast.
Jara M et al. Mol Biol Cell 2007 Jun;18(6):2288-95
PMID:18758731 - The role and regulation of Trxl, a cytosolic thioredoxin in Schizosaccharomyces pombe.
Song JY et al. J Microbiol 2008 Aug;46(4):408-14
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:19625445 - Role for RACK1 orthologue Cpc2 in the modulation of stress response in fission yeast.
Núñez A et al. Mol Biol Cell 2009 Sep;20(18):3996-4009
PMID:28652406 - Deciphering the role of the signal- and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation.
Salat-Canela C et al. J Biol Chem 2017 Aug 18;292(33):13635-13644
PMID:23525001 - Reversible thiol oxidation in the H2O2-dependent activation of the transcription factor Pap1.
Calvo IA et al. J Cell Sci 2013 May 15;126(Pt 10):2279-84
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28640807 - Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.
Boronat S et al. PLoS Genet 2017 Jun;13(6):e1006858
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:11179424 - Peroxide sensors for the fission yeast stress-activated mitogen-activated protein kinase pathway.
Buck V et al. Mol Biol Cell 2001 Feb;12(2):407-19
PMID:9894913 - Cloning of caf1+, caf2+ and caf4+ from Schizosaccharomyces pombe: their involvement in multidrug resistance, UV and pH sensitivity.
Benko Z et al. Mol Gen Genet 1998 Dec;260(5):434-43
PMID:9211790 - Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.
Benko Z et al. Curr Genet 1997 Jun;31(6):481-7
PMID:23121505 - Is oxidized thioredoxin a major trigger for cysteine oxidation? Clues from a redox proteomics approach.
García-Santamarina S et al. Antioxid Redox Signal 2013 May 01;18(13):1549-56
PMID:24268782 - A peroxiredoxin promotes H2O2 signaling and oxidative stress resistance by oxidizing a thioredoxin family protein.
Brown JD et al. Cell Rep 2013 Dec 12;5(5):1425-35
PMID:14758541 - The transcription factor Pap1/Caf3 plays a central role in the determination of caffeine resistance in Schizosaccharomyces pombe.
Benko Z et al. Mol Genet Genomics 2004 Mar;271(2):161-70
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:11071922 - A fission yeast homolog of Int-6, the mammalian oncoprotein and eIF3 subunit, induces drug resistance when overexpressed.
Crane R et al. Mol Biol Cell 2000 Nov;11(11):3993-4003
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24521463 - A genetic approach to study H2O2 scavenging in fission yeast--distinct roles of peroxiredoxin and catalase.
Paulo E et al. Mol Microbiol 2014 Apr;92(2):246-57
PMID:19672306 - Genome-wide screen of genes required for caffeine tolerance in fission yeast.
Calvo IA et al. PLoS One 2009 Aug 12;4(8):e6619
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:16141205 - The glycolytic metabolite methylglyoxal activates Pap1 and Sty1 stress responses in Schizosaccharomyces pombe.
Zuin A et al. J Biol Chem 2005 Nov 04;280(44):36708-13
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:15135546 - Stress-dependent regulation of the gene encoding thioredoxin reductase from the fission yeast.
Hong SM et al. FEMS Microbiol Lett 2004 May 15;234(2):379-85
PMID:9585505 - Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1.
Toone WM et al. Genes Dev 1998 May 15;12(10):1453-63
PMID:10628977 - Isolation and characterization of Nrf1p, a novel negative regulator of the Cdc42p GTPase in Schizosaccharomyces pombe.
Murray JM et al. Genetics 2000 Jan;154(1):155-65
PMID:36174923 - Schizosaccharomyces pombe MAP kinase Sty1 promotes survival of Δppr10 cells with defective mitochondrial protein synthesis.
Hu Y et al. Int J Biochem Cell Biol 2022 Nov;152:106308
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:22279963 - Pap1p-dependent upregulation of thioredoxin 3 and thioredoxin reductase genes from the fission yeast under nitrosative stress.
Park MS et al. Can J Microbiol 2012 Feb;58(2):206-11
PMID:20919928 - Two-component mediated peroxide sensing and signal transduction in fission yeast.
Quinn J et al. Antioxid Redox Signal 2011 Jul 01;15(1):153-65
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:12100563 - Diethylmaleate activates the transcription factor Pap1 by covalent modification of critical cysteine residues.
Castillo EA et al. Mol Microbiol 2002 Jul;45(1):243-54
GO_REF:0000002 - Comments
PMID:10348908 - A fission yeast gene (prr1(+)) that encodes a response regulator implicated in oxidative stress response.
Ohmiya R et al. J Biochem 1999 Jun;125(6):1061-6
PMID:10428959 - Sin1: an evolutionarily conserved component of the eukaryotic SAPK pathway.
Wilkinson MG et al. EMBO J 1999 Aug 02;18(15):4210-21
PMID:18235227 - Fission yeast TOR complex 2 activates the AGC-family Gad8 kinase essential for stress resistance and cell cycle control.
Ikeda K et al. Cell Cycle 2008 Feb 01;7(3):358-64
PMID:22344694 - The transcription factors Pap1 and Prr1 collaborate to activate antioxidant, but not drug tolerance, genes in response to H2O2.
Calvo IA et al. Nucleic Acids Res 2012 Jun;40(11):4816-24
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23115244 - Cells lacking pfh1, a fission yeast homolog of mammalian frataxin protein, display constitutive activation of the iron starvation response.
Gabrielli N et al. J Biol Chem 2012 Dec 14;287(51):43042-51
PMID:16554715 - Transcriptional analysis and pap1-dependence of the unique gene encoding thioredoxin reductase from the fission yeast.
Kang HJ et al. J Microbiol 2006 Feb;44(1):35-41