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protein coding gene - spo14 (SPBC3H7.01) - GEF for Sar1, WD repeat protein Spo14

Gene summary

Standard name
spo14
Systematic ID
SPBC3H7.01
Product
GEF for Sar1, WD repeat protein Spo14
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
stl1, SPBP16F5.01c
UniProt ID
Q10659
ORFeome ID
33/33A12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1889972..1891843 reverse strand

Annotation

Complementation

PBO:0017948 - functionally complemented by S. cerevisiae SEC12

References:

PBO:0012281 - functionally complements S. cerevisiae SEC12

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GO biological process

GO:0032120 - ascospore-type prospore membrane formation

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GO:0048208 - COPII vesicle coat assembly

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GO:0006888 - endoplasmic reticulum to Golgi vesicle-mediated transport

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005783 - endoplasmic reticulum

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GO:0005789 - endoplasmic reticulum membrane

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GO molecular function

GO:0005085 - guanyl-nucleotide exchange factor activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:01148 - ubiquitinylated lysine

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Protein features

PBO:0111751 - WD repeat protein

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000013 - RNA level unchanged

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

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Genotypes:

FYPO:0002711 - abnormal nuclear separation after meiosis

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Genotypes:

FYPO:0001914 - abnormal prospore membrane formation

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Genotypes:

FYPO:0000585 - abolished ascospore wall biogenesis

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Genotypes:

FYPO:0000583 - abolished sporulation

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Genotypes:

FYPO:0003029 - decreased mRNA splicing, via spliceosome

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Genotypes:

FYPO:0001283 - decreased protein level during cellular response to nitrogen starvation

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0003935 - decreased protein localization to Golgi apparatus, with protein mislocalized to endoplasmic reticulum

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Genotypes:

FYPO:0001784 - excess endoplasmic reticulum membrane present

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Genotypes:

FYPO:0003136 - excess plasma membrane present

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Genotypes:

FYPO:0002430 - inviable after spore germination, multiple cell divisions

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0003903 - loss of viability at low temperature

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Genotypes:

FYPO:0000355 - normal endoplasmic reticulum morphology

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Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0003563 - normal meiosis I

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Genotypes:

FYPO:0003798 - normal meiosis II

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Genotypes:

FYPO:0003905 - normal meiotic spindle pole body morphology during meiosis II

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Genotypes:

FYPO:0003904 - normal plasma membrane morphology

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Genotypes:

FYPO:0002332 - normal protein localization to Golgi apparatus

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Genotypes:

FYPO:0003541 - normal protein localization to meiotic spindle pole body

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Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00400WD40WD40_rptPFAM
SM00320WD40_4WD40_rptSMART
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
PTHR23284PROLACTIN REGULATORY ELEMENT BINDING PROTEINSec12-likePANTHER

Orthologs

References / Literature

PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:8203159 - Structural modification of spindle pole bodies during meiosis II is essential for the normal formation of ascospores in Schizosaccharomyces pombe: ultrastructural analysis of spo mutants.
Hirata A et al. Yeast 1994 Feb;10(2):173-83
PMID:3442824 - Genetic mapping of eleven spo genes essential for ascospore formation in the fission yeast Schizosaccharomyces pombe.
Kishida M et al. Curr Genet 1986;10(6):443-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:12631727 - The fission yeast spo14+ gene encoding a functional homologue of budding yeast Sec12 is required for the development of forespore membranes.
Nakamura-Kubo M et al. Mol Biol Cell 2003 Mar;14(3):1109-24
PMID:1396601 - Fission yeast and a plant have functional homologues of the Sar1 and Sec12 proteins involved in ER to Golgi traffic in budding yeast.
d'Enfert C et al. EMBO J 1992 Nov;11(11):4205-11
PMID:18550796 - Live observation of forespore membrane formation in fission yeast.
Nakamura T et al. Mol Biol Cell 2008 Aug;19(8):3544-53
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:1417417 - Electron microscopic examination of sporulation-deficient mutants of the fission yeast Schizosaccharomyces pombe.
Hirata A et al. Arch Microbiol 1992;158(4):249-55
PMID:16272747 - A fission yeast SNAP-25 homologue, SpSec9, is essential for cytokinesis and sporulation.
Nakamura T et al. Cell Struct Funct 2005;30(2):15-24
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PANTHER:PTHR23284 - Unknown title
PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.
Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6