protein coding gene - mcm2 (SPBC4.04c) - MCM complex subunit Mcm2

Gene summary

Standard name

mcm2

Systematic ID

SPBC4.04c

Product

MCM complex subunit Mcm2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

cdc19, nda1

UniProt ID

P40377

ORFeome ID

33/33H09

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 1191208..1194142 reverse strand

Annotation

Protein features

Orthologs

• MCM2 (HGNC:6944) Homo sapiens
• mcm2 (SJAG_02348) Schizosaccharomyces japonicus
• MCM2 (YBL023C) Saccharomyces cerevisiae

References / Literature

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:12574132 - Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeast.

Takahashi T et al. EMBO J 2003 Feb 17;22(4):964-74

PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.

Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433

PMID:10490657 - Association of fission yeast Orp1 and Mcm6 proteins with chromosomal replication origins.

Ogawa Y et al. Mol Cell Biol 1999 Oct;19(10):7228-36

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:9679144 - Regulation of cell polarity by microtubules in fission yeast.

Sawin KE et al. J Cell Biol 1998 Jul 27;142(2):457-71

PMID:21813639 - Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.

Ding L et al. J Biol Chem 2011 Sep 23;286(38):32918-30

PMID:38285941 - Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.

Nathanailidou P et al. Proc Natl Acad Sci U S A 2024 Feb 06;121(6):e2315596121

PMID:8298187 - Fission yeast genes nda1+ and nda4+, mutations of which lead to S-phase block, chromatin alteration and Ca2+ suppression, are members of the CDC46/MCM2 family.

Miyake S et al. Mol Biol Cell 1993 Oct;4(10):1003-15

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:6943408 - Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe.

Nasmyth K et al. Mol Gen Genet 1981;182(1):119-24

PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.

Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20

PMID:16899242 - Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast.

Namdar M et al. Exp Cell Res 2006 Oct 15;312(17):3360-9

PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80

GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:10409743 - A fission yeast gene, him1(+)/dfp1(+), encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S-phase initiation as well as in S-phase checkpoint control and recovery from DNA damage.

Takeda T et al. Mol Cell Biol 1999 Aug;19(8):5535-47

PMID:11402029 - Bipartite binding of a kinase activator activates Cdc7-related kinase essential for S phase.

Ogino K et al. J Biol Chem 2001 Aug 17;276(33):31376-87

PMID:9914167 - Reduced dosage of a single fission yeast MCM protein causes genetic instability and S phase delay.

Liang DT et al. J Cell Sci 1999 Feb;112 ( Pt 4):559-67

PMID:10388806 - Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes.

Snaith HA et al. Genetics 1999 Jul;152(3):839-51

PMID:10725227 - A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls.

Grallert B et al. J Cell Sci 2000 Apr;113 ( Pt 8):1447-58

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:38479839 - Coordination of histone chaperones for parental histone segregation and epigenetic inheritance.

Fang Y et al. Genes Dev 2024 Mar 13;

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:11973289 - Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteins.

Gómez EB et al. Genetics 2002 Apr;160(4):1305-18

PMID:15194812 - A novel intermediate in initiation complex assembly for fission yeast DNA replication.

Yamada Y et al. Mol Biol Cell 2004 Aug;15(8):3740-50

PMID:21099360 - Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.

Matsumoto S et al. Cell Cycle 2010 Dec 01;9(23):4627-37

PMID:20176980 - Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.

Dolan WP et al. Genetics 2010 May;185(1):39-53

PMID:18180284 - Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stability.

Bailis JM et al. Mol Cell Biol 2008 Mar;28(5):1724-38

PMID:22433840 - Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.

Kanke M et al. EMBO J 2012 May 02;31(9):2182-94

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:22024164 - Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.

Matsumoto S et al. J Cell Biol 2011 Oct 31;195(3):387-401

PMID:41786738 - An H3K14ub-H3K9me3 feedback circuit governs heterochromatin spreading and inheritance in fission yeast.

Toda T et al. Nat Commun 2026 Mar 05;

PMID:7876346 - The fission yeast cdc19+ gene encodes a member of the MCM family of replication proteins.

Forsburg SL et al. J Cell Sci 1994 Oct;107 ( Pt 10):2779-88

PMID:9658174 - Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complex.

Sherman DA et al. Mol Biol Cell 1998 Jul;9(7):1833-45

PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238

PMID:9705352 - Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast.

Brown GW et al. J Biol Chem 1998 Aug 21;273(34):22083-90

PMID:14508607 - Ibp1p, a novel Cdc25-related phosphatase, suppresses Schizosaccharomyces pombe hsk1 ( cdc7).

Snaith HA et al. Curr Genet 2003 Oct;44(1):38-48

PMID:9383050 - Mutational analysis of Cdc19p, a Schizosaccharomyces pombe MCM protein.

Forsburg SL et al. Genetics 1997 Nov;147(3):1025-41

PMID:11027263 - Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity.

Snaith HA et al. Mol Cell Biol 2000 Nov;20(21):7922-32

PMID:16263721 - Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex.

Matsumoto S et al. J Biol Chem 2005 Dec 30;280(52):42536-42

PMID:12419251 - Stable association of mitotic cyclin B/Cdc2 to replication origins prevents endoreduplication.

Wuarin J et al. Cell 2002 Nov 01;111(3):419-31

PMID:26368543 - Critical Function of γH2A in S-Phase.

Mejia-Ramirez E et al. PLoS Genet 2015 Sep;11(9):e1005517

PMID:8838655 - Fission yeast Nda1 and Nda4, MCM homologs required for DNA replication, are constitutive nuclear proteins.

Okishio N et al. J Cell Sci 1996 Feb;109 ( Pt 2):319-26

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:12972571 - Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding.

Gregan J et al. Mol Biol Cell 2003 Sep;14(9):3876-87

PMID:11523776 - Sna41goa1, a novel mutation causing G1/S arrest in fission yeast, is defective in a CDC45 homolog and interacts genetically with polalpha.

Uchiyama M et al. Mol Genet Genomics 2001 Aug;265(6):1039-49

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:11359920 - Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast.

Takeda T et al. Mol Biol Cell 2001 May;12(5):1257-74

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:39094566 - Mrc1 regulates parental histone segregation and heterochromatin inheritance.

Toda T et al. Mol Cell 2024 Jul 23;

PMID:16849602 - Regulation of DNA replication machinery by Mrc1 in fission yeast.

Nitani N et al. Genetics 2006 Sep;174(1):155-65

PMID:23349636 - Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.

Roseaulin LC et al. PLoS Genet 2013;9(1):e1003213

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:11854402 - Essential role of MCM proteins in premeiotic DNA replication.

Lindner K et al. Mol Biol Cell 2002 Feb;13(2):435-44

PMID:12604790 - The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase.

Lee JK et al. Proc Natl Acad Sci U S A 2003 Mar 04;100(5):2334-9

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:9891039 - Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion.

Tanaka H et al. Mol Cell Biol 1999 Feb;19(2):1038-48

PMID:18077559 - Biphasic incorporation of centromeric histone CENP-A in fission yeast.

Takayama Y et al. Mol Biol Cell 2008 Feb;19(2):682-90

PMID:10588642 - Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly.

Pasion SG et al. Mol Biol Cell 1999 Dec;10(12):4043-57

PMID:6887244 - Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombe.

Toda T et al. J Mol Biol 1983 Aug 05;168(2):251-70

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:10888871 - Mitotic replication initiation proteins are not required for pre-meiotic S phase.

Forsburg SL et al. Nat Genet 2000 Jul;25(3):263-8

GO_REF:0000002 - Comments

PMID:22036784 - Purification and functional inactivation of the fission yeast MCM(MCM-BP) complex.

Li JJ et al. FEBS Lett 2011 Dec 15;585(24):3850-5

PMID:9693370 - Mutational effect of fission yeast polalpha on cell cycle events.

Bhaumik D et al. Mol Biol Cell 1998 Aug;9(8):2107-23

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:18505873 - Schizosaccharomyces pombe histone acetyltransferase Mst1 (KAT5) is an essential protein required for damage response and chromosome segregation.

Gómez EB et al. Genetics 2008 Jun;179(2):757-71

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:11606526 - Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.

Liang DT et al. Genetics 2001 Oct;159(2):471-86

PMID:12411492 - Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle.

Anderson M et al. EMBO J 2002 Nov 01;21(21):5745-55

PMID:12185500 - Fission yeast Cdc23 interactions with DNA replication initiation proteins.

Hart EA et al. Curr Genet 2002 Aug;41(5):342-8

PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.

Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24

PMID:40861011 - Faithful inheritance: Parental histone recycling and epigenetic memory in fission yeast.

Fang Y et al. Cell Insight 2025 Oct;4(5):100275

PMID:9366552 - A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast.

Adachi Y et al. Genes Cells 1997 Jul;2(7):467-79

PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.

Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82

PMID:10411894 - Cell cycle regulation of Dfp1, an activator of the Hsk1 protein kinase.

Brown GW et al. Proc Natl Acad Sci U S A 1999 Jul 20;96(15):8443-8

PMID:18057023 - Regulation of gene expression during M-G1-phase in fission yeast through Plo1p and forkhead transcription factors.

Papadopoulou K et al. J Cell Sci 2008 Jan 01;121(Pt 1):38-47

PMID:9619628 - Identification of sna41 gene, which is the suppressor of nda4 mutation and is involved in DNA replication in Schizosaccharomyces pombe.

Miyake S et al. Genes Cells 1998 Mar;3(3):157-66

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:21593208 - CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.

Fukuura M et al. Mol Biol Cell 2011 Jul 15;22(14):2620-33

PMID:10770926 - Isolation and characterization of various complexes of the minichromosome maintenance proteins of Schizosaccharomyces pombe.

Lee JK et al. J Biol Chem 2000 Jun 23;275(25):18871-8