protein coding gene - rpt2 (SPBC4.07c) - 19S proteasome base subcomplex ATPase subunit Rpt2

Gene summary

Standard name

rpt2

Systematic ID

SPBC4.07c

Product

19S proteasome base subcomplex ATPase subunit Rpt2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

mts2

UniProt ID

P36612

ORFeome ID

40/40E11

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 1197778..1199428 reverse strand

Annotation

Protein features

Orthologs

• PSMC1 (HGNC:9547) Homo sapiens
• rpt2 (SJAG_00712) Schizosaccharomyces japonicus
• RPT2 (YDL007W) Saccharomyces cerevisiae

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:38181050 - The Cross-Regulation Between Set1, Clr4, and Lsd1/2 in Schizosaccharomyces pombe.

Liu H et al. PLoS Genet 2024 Jan 05;20(1):e1011107

PMID:28765280 - The exocyst subunit Sec3 is regulated by a protein quality control pathway.

Kampmeyer C et al. J Biol Chem 2017 Sep 15;292(37):15240-15253

PMID:12006658 - Sum1, a component of the fission yeast eIF3 translation initiation complex, is rapidly relocalized during environmental stress and interacts with components of the 26S proteasome.

Dunand-Sauthier I et al. Mol Biol Cell 2002 May;13(5):1626-40

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:28031482 - Involvement of fission yeast Pdc2 in RNA degradation and P-body function.

Wang CY et al. RNA 2017 Apr;23(4):493-503

PMID:22144913 - Widespread cotranslational formation of protein complexes.

Duncan CD et al. PLoS Genet 2011 Dec;7(12):e1002398

PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.

Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99

PMID:38285941 - Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.

Nathanailidou P et al. Proc Natl Acad Sci U S A 2024 Feb 06;121(6):e2315596121

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:15533439 - Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast.

Stone M et al. J Mol Biol 2004 Nov 26;344(3):697-706

PMID:20231270 - Schizosaccharomyces pombe Dss1p is a DNA damage checkpoint protein that recruits Rad24p, Cdc25p, and Rae1p to DNA double-strand breaks.

Selvanathan SP et al. J Biol Chem 2010 May 07;285(19):14122-33

PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.

Larochelle M et al. J Cell Sci 2019 May 31;132(11)

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:21098295 - Structure of the 26S proteasome from Schizosaccharomyces pombe at subnanometer resolution.

Bohn S et al. Proc Natl Acad Sci U S A 2010 Dec 07;107(49):20992-7

PMID:11084332 - Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.

Tatebe H et al. Curr Biol 2000 Nov 02;10(21):1329-38

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:8621436 - A conditional lethal mutant in the fission yeast 26 S protease subunit mts3+ is defective in metaphase to anaphase transition.

Gordon C et al. J Biol Chem 1996 Mar 08;271(10):5704-11

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:12615927 - Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins.

Seeger M et al. J Biol Chem 2003 May 09;278(19):16791-6

PMID:20668161 - Proteasome nuclear import mediated by Arc3 can influence efficient DNA damage repair and mitosis in Schizosaccharomyces pombe.

Cabrera R et al. Mol Biol Cell 2010 Sep 15;21(18):3125-36

PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.

Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:9325304 - Mts4, a non-ATPase subunit of the 26 S protease in fission yeast is essential for mitosis and interacts directly with the ATPase subunit Mts2.

Wilkinson CR et al. J Biol Chem 1997 Oct 10;272(41):25768-77

PMID:34608864 - DDK/Hsk1 phosphorylates and targets fission yeast histone deacetylase Hst4 for degradation to stabilize stalled DNA replication forks.

Aricthota S et al. Elife 2021 Oct 05;10

GO_REF:0000051 - S. pombe keyword mapping

PMID:24741065 - S. pombe TORC1 activates the ubiquitin-proteasomal degradation of the meiotic regulator Mei2 in cooperation with Pat1 kinase.

Otsubo Y et al. J Cell Sci 2014 Jun 15;127(Pt 12):2639-46

PMID:16149916 - Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis.

Jossé L et al. Biochem J 2006 Jan 01;393(Pt 1):303-9

PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.

Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70

GO_REF:0000002 - Comments

PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.

Morozumi Y et al. iScience 2024 Jan 19;27(1):108777

PMID:28841135 - Ubiquitination-dependent control of sexual differentiation in fission yeast.

Simonetti F et al. Elife 2017 Aug 25;6

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608

PMID:23348717 - Essential role of Ubr11, but not Ubr1, as an N-end rule ubiquitin ligase in Schizosaccharomyces pombe.

Fujiwara H et al. Yeast 2013 Jan;30(1):1-11

PMID:37694715 - A ubiquitin-proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis.

Le TK et al. J Cell Sci 2023 Oct 01;136(19)

PMID:38272226 - E3 ubiquitin ligase Hul6 modulates iron-dependent metabolism by regulating Php4 stability.

Yao R et al. J Biol Chem 2024 Jan 23;:105670

PMID:24710126 - The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin.

Kitagawa T et al. Nat Commun 2014 Apr 07;5:3597

PMID:11683390 - In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis.

Decottignies A et al. J Cell Sci 2001 Jul;114(Pt 14):2627-40

PMID:18023413 - Dss1 associating with the proteasome functions in selective nuclear mRNA export in yeast.

Mannen T et al. Biochem Biophys Res Commun 2008 Jan 25;365(4):664-71

PMID:20133687 - Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast.

Takeda K et al. Proc Natl Acad Sci U S A 2010 Feb 23;107(8):3540-5

PMID:20838651 - A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity.

Kouranti I et al. PLoS Biol 2010 Sep 07;8(9)

PMID:33574613 - TOR targets an RNA processing network to regulate facultative heterochromatin, developmental gene expression and cell proliferation.

Wei Y et al. Nat Cell Biol 2021 Mar;23(3):243-256

PMID:8247131 - Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit.

Gordon C et al. Nature 1993 Nov 25;366(6453):355-7

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:8918598 - Characteristics of 26 S proteases from fission yeast mutants, which arrest in mitosis.

Seeger M et al. J Mol Biol 1996 Nov 01;263(3):423-31

PMID:21324894 - Nuclear protein quality is regulated by the ubiquitin-proteasome system through the activity of Ubc4 and San1 in fission yeast.

Matsuo Y et al. J Biol Chem 2011 Apr 15;286(15):13775-90

PMID:12553909 - Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome.

Yen HC et al. Cell 2003 Jan 24;112(2):207-17

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

PMID:24938783 - Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes.

Leitner A et al. Proc Natl Acad Sci U S A 2014 Jul 01;111(26):9455-60

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:23209828 - Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor.

Penney M et al. PLoS One 2012;7(11):e50796

PMID:24497846 - A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.

Kriegenburg F et al. PLoS Genet 2014 Jan;10(1):e1004140

PMID:28784663 - The 19S proteasome is directly involved in the regulation of heterochromatin spreading in fission yeast.

Seo HD et al. J Biol Chem 2017 Oct 13;292(41):17144-17155

PMID:12783882 - Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly.

Yen HC et al. J Biol Chem 2003 Aug 15;278(33):30669-76

PMID:20976105 - Silencing mediated by the Schizosaccharomyces pombe HIRA complex is dependent upon the Hpc2-like protein, Hip4.

Anderson HE et al. PLoS One 2010 Oct 18;5(10):e13488

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:22307589 - Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach.

Lasker K et al. Proc Natl Acad Sci U S A 2012 Jan 31;109(5):1380-7

PMID:9653157 - sud1(+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast.

Jallepalli PV et al. Proc Natl Acad Sci U S A 1998 Jul 07;95(14):8159-64

PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.

Calvo IA et al. iScience 2021 Nov 19;24(11):103338