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protein coding gene - swi5 (SPBC409.03) - DNA recombination mediator Swi5

Gene summary

Standard name
swi5
Systematic ID
SPBC409.03
Product
DNA recombination mediator Swi5
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UUB7
ORFeome ID
42/42B08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1137907..1139030 forward strand

Annotation

Comment

PBO:0091490 - accumulation and persistence of Swi5 foci in rad54delta cells

References:

PBO:0091712 - facilitates Rad51 assembly at damage sites independently of Rad57

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PBO:0003493 - focus formation at DNA damage sites, Swi5_dependent

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PBO:0003492 - focus formation at heterochromatic regions, Swi2_dependent

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PBO:0091368 - increased frequency of long tract gene conversion in the rad57delta single mutant was suppressed in the swi5delta rad57delta double mutant, to a level similar to that observed in the rad51 mutant

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PBO:0091367 - swi5delta rad57delta double mutant produced each recombination class (gene conversion, crossover and long tract gene conversion) at frequencies similar to that of the rad51delta single mutant

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PBO:0091369 - swi5delta rad57delta double mutant showed a repair profile very similar to that of the rad51delta single mutant

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GO biological process

GO:0000730 - DNA recombinase assembly

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GO:0006310 - DNA recombination

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GO:0042148 - DNA strand invasion

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GO:0000724 - double-strand break repair via homologous recombination

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GO:0007534 - gene conversion at mating-type locus

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GO:0007533 - mating type switching

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GO:0010772 - meiotic DNA recombinase assembly involved in reciprocal meiotic recombination

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GO:0000709 - meiotic joint molecule formation

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GO:0000708 - meiotic strand invasion

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GO cellular component

GO:0031934 - mating-type region heterochromatin

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GO:0005634 - nucleus

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GO:0032798 - Swi5-Sfr1 complex

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GO:0034974 - Swi5-Swi2 complex

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GO molecular function

GO:0001671 - ATPase activator activity

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GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0003913 - decreased minichromosome loss during vegetative growth

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Genotypes:

FYPO:0000316 - inviable after spore germination

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Genotypes:

FYPO:0000472 - normal mating type switching

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0003612 - viable spore population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0005139 - abnormal meiotic recombination double-strand break repair

References:

Genotypes:

FYPO:0000469 - abolished mating type switching

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Genotypes:

FYPO:0000185 - decreased gene conversion during vegetative growth

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Genotypes:

FYPO:0003179 - decreased intragenic meiotic recombination

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0000581 - decreased spore germination frequency

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000199 - increased long tract gene conversion

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Genotypes:

FYPO:0001821 - increased number of heterothallic h+ cells

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0003353 - normal DNA double-strand break formation at mating-type locus

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Genotypes:

FYPO:0005136 - normal meiotic DNA double-strand break formation

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Genotypes:

FYPO:0004437 - normal mitotic recombination frequency

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Genotypes:

FYPO:0003530 - normal S-phase DNA damage checkpoint

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0000678 - unequal homologous chromosome segregation

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Genotypes:

FYPO:0003612 - viable spore population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Subunit composition

PBO:0015230 - heteromeric(3)

References:

PBO:0017751 - heteromeric(4)

References:

PBO:0015242 - homomeric(4)

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Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF07061Swi5DNA-repair_Swi5Pfam
G3DSA:1.20.5.170:FF:000056CATH-FunFam
G3DSA:1.20.5.170CATH-Gene3D
PTHR28529DNA-repair_Swi5PANTHER
CoilCOILS

Orthologs

References / Literature

PMID:15238514 - Conserved and nonconserved proteins for meiotic DNA breakage and repair in yeasts.
Young JA et al. Genetics 2004 Jun;167(2):593-605
PMID:16135800 - The F-Box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteins.
Osman F et al. Mol Cell Biol 2005 Sep;25(18):8084-96
PMID:14663140 - Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast.
Akamatsu Y et al. Proc Natl Acad Sci U S A 2003 Dec 23;100(26):15770-5
PMID:23324799 - Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering.
Saikusa K et al. Analyst 2013 Mar 07;138(5):1441-9
PMID:15802523 - A postsynaptic role for Rhp55/57 that is responsible for cell death in Deltarqh1 mutants following replication arrest in Schizosaccharomyces pombe.
Hope JC et al. Genetics 2005 Jun;170(2):519-31
PMID:15972456 - Brc1-mediated DNA repair and damage tolerance.
Sheedy DM et al. Genetics 2005 Oct;171(2):457-68
PMID:22042869 - A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination.
Matsuda E et al. Proc Natl Acad Sci U S A 2011 Nov 15;108(46):18754-9
PMID:24719968 - Switching genes in Schizosaccharomyces pombe.
Gutz H et al. Curr Genet 1985;9(5):325-31
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23828040 - Involvement of Schizosaccharomyces pombe rrp1+ and rrp2+ in the Srs2- and Swi5/Sfr1-dependent pathway in response to DNA damage and replication inhibition.
Dziadkowiec D et al. Nucleic Acids Res 2013 Sep;41(17):8196-209
PMID:29323270 - Two three-strand intermediates are processed during Rad51-driven DNA strand exchange.
Ito K et al. Nat Struct Mol Biol 2018 Jan;25(1):29-36
PMID:15466419 - Swi5 acts in meiotic DNA joint molecule formation in Schizosaccharomyces pombe.
Ellermeier C et al. Genetics 2004 Dec;168(4):1891-8
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:18388861 - Mus81 is essential for sister chromatid recombination at broken replication forks.
Roseaulin L et al. EMBO J 2008 May 07;27(9):1378-87
PMID:30297419 - Swi5-Sfr1 stimulates Rad51 recombinase filament assembly by modulating Rad51 dissociation.
Lu CH et al. Proc Natl Acad Sci U S A 2018 Oct 23;115(43):E10059-E10068
PMID:34208949 - Mapping and Analysis of Swi5 and Sfr1 Phosphorylation Sites.
Sevcovicova A et al. Genes (Basel) 2021 Jun 30;12(7)
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:2598273 - Some of the swi genes of Schizosaccharomyces pombe also have a function in the repair of radiation damage.
Schmidt H et al. Curr Genet 1989 Aug;16(2):89-94
PMID:10716938 - Fission yeast switches mating type by a replication-recombination coupled process.
Arcangioli B et al. EMBO J 2000 Mar 15;19(6):1389-96
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:16478984 - The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.
Pebernard S et al. Mol Cell Biol 2006 Mar;26(5):1617-30
PMID:18354497 - The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching.
Aguilar-Arnal L et al. EMBO J 2008 Apr 09;27(7):1029-38
PMID:22405003 - Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex.
Kuwabara N et al. Structure 2012 Mar 07;20(3):440-9
PMID:24186976 - Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition.
Murayama Y et al. Genes Dev 2013 Nov 01;27(21):2299-304
PMID:1874411 - Isolation and characterization of Schizosaccharomyces pombe mutants affected in mitotic recombination.
Gysler-Junker A et al. Genetics 1991 Jul;128(3):495-504
PMID:36951094 - Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching.
Maki T et al. Nucleic Acids Res 2023 May 08;51(8):3869-3887
PMID:9136000 - The Schizosaccharomyces pombe rec16 gene product regulates multiple meiotic events.
Li YF et al. Genetics 1997 May;146(1):57-67
PMID:16921379 - The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.
Haruta N et al. Nat Struct Mol Biol 2006 Sep;13(9):823-30
PMID:32414915 - Two auxiliary factors promote Dmc1-driven DNA strand exchange via stepwise mechanisms.
Tsubouchi H et al. Proc Natl Acad Sci U S A 2020 Jun 02;117(22):12062-12070
PMID:32204793 - Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex.
Argunhan B et al. Elife 2020 Mar 24;9
PMID:38673778 - Dysfunction of Gpl1-Gih35-Wdr83 Complex in S. pombe Affects the Splicing of DNA Damage Repair Factors Resulting in Increased Sensitivity to DNA Damage.
Cipakova I et al. Int J Mol Sci 2024 Apr 10;25(8)
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:20823543 - Expression, purification and crystallization of Swi5 and the Swi5-Sfr1 complex from fission yeast.
Kuwabara N et al. Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Sep 01;66(Pt 9):1124-6
PMID:17353272 - Mus81-Eme1-dependent and -independent crossovers form in mitotic cells during double-strand break repair in Schizosaccharomyces pombe.
Hope JC et al. Mol Cell Biol 2007 May;27(10):3828-38
PMID:18416603 - Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators.
Kurokawa Y et al. PLoS Biol 2008 Apr 15;6(4):e88
PMID:37330173 - Phosphoregulation of DNA repair via the Rad51 auxiliary factor Swi5-Sfr1.
Liang P et al. J Biol Chem 2023 Aug;299(8):104929
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25165823 - Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
Tsutsui Y et al. PLoS Genet 2014 Aug;10(8):e1004542
PMID:39340300 - The Swi5-Sfr1 complex regulates Dmc1- and Rad51-driven DNA strand exchange proceeding through two distinct three-stranded intermediates by different mechanisms.
Ito K et al. Nucleic Acids Res 2024 Sep 28;
PMID:20885790 - Critical functions of Rpa3/Ssb3 in S-phase DNA damage responses in fission yeast.
Cavero S et al. PLoS Genet 2010 Sep 23;6(9):e1001138
PMID:15537537 - Heterochromatin regulates cell type-specific long-range chromatin interactions essential for directed recombination.
Jia S et al. Cell 2004 Nov 12;119(4):469-80
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20655467 - Crossover invariance determined by partner choice for meiotic DNA break repair.
Hyppa RW et al. Cell 2010 Jul 23;142(2):243-55
PMID:17304215 - Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.
Akamatsu Y et al. EMBO J 2007 Mar 07;26(5):1352-62
PMID:22033972 - Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure.
Kokabu Y et al. J Biol Chem 2011 Dec 16;286(50):43569-76
PMID:19037101 - Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.
Willis N et al. Mol Biol Cell 2009 Feb;20(3):819-33
PMID:25293972 - Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).
Mastro TL et al. Genetics 2014 Dec;198(4):1457-72
PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.
Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69
PMID:6587363 - Genes required for initiation and resolution steps of mating-type switching in fission yeast.
Egel R et al. Proc Natl Acad Sci U S A 1984 Jun;81(11):3481-5
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:9710635 - A novel function of the DNA repair gene rhp6 in mating-type silencing by chromatin remodeling in fission yeast.
Singh J et al. Mol Cell Biol 1998 Sep;18(9):5511-22