protein coding gene - swi5 (SPBC409.03) - DNA recombination mediator Swi5

Gene summary

Standard name

swi5

Systematic ID

SPBC409.03

Product

DNA recombination mediator Swi5

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q9UUB7

ORFeome ID

42/42B08

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 1137907..1139030 forward strand

Annotation

Protein features

Orthologs

• SWI5 (HGNC:31412) Homo sapiens
• swi5 (SJAG_03221) Schizosaccharomyces japonicus
• SAE3 (YHR079C-A) Saccharomyces cerevisiae

References / Literature

PMID:16135800 - The F-Box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteins.

Osman F et al. Mol Cell Biol 2005 Sep;25(18):8084-96

PMID:15238514 - Conserved and nonconserved proteins for meiotic DNA breakage and repair in yeasts.

Young JA et al. Genetics 2004 Jun;167(2):593-605

PMID:20655467 - Crossover invariance determined by partner choice for meiotic DNA break repair.

Hyppa RW et al. Cell 2010 Jul 23;142(2):243-55

PMID:16478984 - The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.

Pebernard S et al. Mol Cell Biol 2006 Mar;26(5):1617-30

PMID:20823543 - Expression, purification and crystallization of Swi5 and the Swi5-Sfr1 complex from fission yeast.

Kuwabara N et al. Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Sep 01;66(Pt 9):1124-6

PMID:19037101 - Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.

Willis N et al. Mol Biol Cell 2009 Feb;20(3):819-33

PMID:24186976 - Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition.

Murayama Y et al. Genes Dev 2013 Nov 01;27(21):2299-304

PMID:29323270 - Two three-strand intermediates are processed during Rad51-driven DNA strand exchange.

Ito K et al. Nat Struct Mol Biol 2018 Jan;25(1):29-36

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:17304215 - Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.

Akamatsu Y et al. EMBO J 2007 Mar 07;26(5):1352-62

PMID:30297419 - Swi5-Sfr1 stimulates Rad51 recombinase filament assembly by modulating Rad51 dissociation.

Lu CH et al. Proc Natl Acad Sci U S A 2018 Oct 23;115(43):E10059-E10068

PMID:2598273 - Some of the swi genes of Schizosaccharomyces pombe also have a function in the repair of radiation damage.

Schmidt H et al. Curr Genet 1989 Aug;16(2):89-94

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:15802523 - A postsynaptic role for Rhp55/57 that is responsible for cell death in Deltarqh1 mutants following replication arrest in Schizosaccharomyces pombe.

Hope JC et al. Genetics 2005 Jun;170(2):519-31

PMID:15466419 - Swi5 acts in meiotic DNA joint molecule formation in Schizosaccharomyces pombe.

Ellermeier C et al. Genetics 2004 Dec;168(4):1891-8

PMID:32204793 - Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex.

Argunhan B et al. Elife 2020 Mar 24;9

PMID:18416603 - Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators.

Kurokawa Y et al. PLoS Biol 2008 Apr 15;6(4):e88

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:23828040 - Involvement of Schizosaccharomyces pombe rrp1+ and rrp2+ in the Srs2- and Swi5/Sfr1-dependent pathway in response to DNA damage and replication inhibition.

Dziadkowiec D et al. Nucleic Acids Res 2013 Sep;41(17):8196-209

PMID:32414915 - Two auxiliary factors promote Dmc1-driven DNA strand exchange via stepwise mechanisms.

Tsubouchi H et al. Proc Natl Acad Sci U S A 2020 Jun 02;117(22):12062-12070

PMID:22042869 - A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination.

Matsuda E et al. Proc Natl Acad Sci U S A 2011 Nov 15;108(46):18754-9

PMID:39340300 - The Swi5-Sfr1 complex regulates Dmc1- and Rad51-driven DNA strand exchange proceeding through two distinct three-stranded intermediates by different mechanisms.

Ito K et al. Nucleic Acids Res 2024 Sep 28;

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:15972456 - Brc1-mediated DNA repair and damage tolerance.

Sheedy DM et al. Genetics 2005 Oct;171(2):457-68

PMID:1874411 - Isolation and characterization of Schizosaccharomyces pombe mutants affected in mitotic recombination.

Gysler-Junker A et al. Genetics 1991 Jul;128(3):495-504

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:18354497 - The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching.

Aguilar-Arnal L et al. EMBO J 2008 Apr 09;27(7):1029-38

PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.

Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69

PMID:16921379 - The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.

Haruta N et al. Nat Struct Mol Biol 2006 Sep;13(9):823-30

PMID:36951094 - Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching.

Maki T et al. Nucleic Acids Res 2023 May 08;51(8):3869-3887

PMID:17353272 - Mus81-Eme1-dependent and -independent crossovers form in mitotic cells during double-strand break repair in Schizosaccharomyces pombe.

Hope JC et al. Mol Cell Biol 2007 May;27(10):3828-38

PMID:25165823 - Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.

Tsutsui Y et al. PLoS Genet 2014 Aug;10(8):e1004542

PMID:9710635 - A novel function of the DNA repair gene rhp6 in mating-type silencing by chromatin remodeling in fission yeast.

Singh J et al. Mol Cell Biol 1998 Sep;18(9):5511-22

PMID:22033972 - Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure.

Kokabu Y et al. J Biol Chem 2011 Dec 16;286(50):43569-76

PMID:15537537 - Heterochromatin regulates cell type-specific long-range chromatin interactions essential for directed recombination.

Jia S et al. Cell 2004 Nov 12;119(4):469-80

PMID:22405003 - Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex.

Kuwabara N et al. Structure 2012 Mar 07;20(3):440-9

PMID:10716938 - Fission yeast switches mating type by a replication-recombination coupled process.

Arcangioli B et al. EMBO J 2000 Mar 15;19(6):1389-96

PMID:20885790 - Critical functions of Rpa3/Ssb3 in S-phase DNA damage responses in fission yeast.

Cavero S et al. PLoS Genet 2010 Sep 23;6(9):e1001138

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:23324799 - Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering.

Saikusa K et al. Analyst 2013 Mar 07;138(5):1441-9

PMID:37330173 - Phosphoregulation of DNA repair via the Rad51 auxiliary factor Swi5-Sfr1.

Liang P et al. J Biol Chem 2023 Aug;299(8):104929

PMID:14663140 - Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast.

Akamatsu Y et al. Proc Natl Acad Sci U S A 2003 Dec 23;100(26):15770-5

PMID:24719968 - Switching genes in Schizosaccharomyces pombe.

Gutz H et al. Curr Genet 1985;9(5):325-31

PMID:34208949 - Mapping and Analysis of Swi5 and Sfr1 Phosphorylation Sites.

Sevcovicova A et al. Genes (Basel) 2021 Jun 30;12(7)

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:18388861 - Mus81 is essential for sister chromatid recombination at broken replication forks.

Roseaulin L et al. EMBO J 2008 May 07;27(9):1378-87

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:6587363 - Genes required for initiation and resolution steps of mating-type switching in fission yeast.

Egel R et al. Proc Natl Acad Sci U S A 1984 Jun;81(11):3481-5

PMID:25293972 - Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).

Mastro TL et al. Genetics 2014 Dec;198(4):1457-72

PMID:9136000 - The Schizosaccharomyces pombe rec16 gene product regulates multiple meiotic events.

Li YF et al. Genetics 1997 May;146(1):57-67