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protein coding gene - rib4 (SPBC409.13) - 6,7-dimethyl-8-ribityllumazine synthase

Gene summary

Standard name
rib4
Systematic ID
SPBC409.13
Product
6,7-dimethyl-8-ribityllumazine synthase
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UUB1
ORFeome ID
09/09E04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1165414..1166729 forward strand

Annotation

GO biological process

GO:0009231 - riboflavin biosynthetic process

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005758 - mitochondrial intermembrane space

References:

GO:0005634 - nucleus

References:

GO:0009349 - riboflavin synthase complex

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GO molecular function

GO:0000906 - 6,7-dimethyl-8-ribityllumazine synthase activity

References:

GO:0005515 - protein binding

References:

GO:1902444 - riboflavin binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002785 - abolished riboflavin binding

References:

Genotypes:

FYPO:0002809 - decreased 6,7-dimethyl-8-ribityllumazine synthase activity

References:

Genotypes:

FYPO:0002786 - decreased riboflavin binding

References:

Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Subunit composition

PBO:0015247 - homomeric(5)

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Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00885DMRL_synthaseLS/RSPfam
cd09209Lumazine_synthase-ILSCDD
G3DSA:3.40.50.960:FF:000007CATH-FunFam
G3DSA:3.40.50.960LS/RS_sfCATH-Gene3D
SSF52121LS/RS_sfSUPERFAMILY
PTHR21058LSPANTHER
TIGR00114lumazine-synthLSNCBIFAM
MF_00178Lumazine_synthLSHAMAP

Orthologs

References / Literature

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:12083520 - The structural basis of riboflavin binding to Schizosaccharomyces pombe 6,7-dimethyl-8-ribityllumazine synthase.
Gerhardt S et al. J Mol Biol 2002 May 17;318(5):1317-29
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:11856310 - Biosynthesis of riboflavin: 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe.
Fischer M et al. Eur J Biochem 2002 Jan;269(2):519-26
GO_REF:0000002 - Comments
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:15265040 - Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase.
Koch M et al. Eur J Biochem 2004 Aug;271(15):3208-14
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623