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protein coding gene - pho4 (SPBC428.03c) - extracellular thiamine-repressible acid phosphatase Pho4

Gene summary

Standard name
pho4
Systematic ID
SPBC428.03c
Product
extracellular thiamine-repressible acid phosphatase Pho4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q01682
ORFeome ID
21/21E07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 445324..447028 reverse strand

Annotation

PBO:0002267 - 3.1.3.2

Disease association

MONDO:0030438 - pontocerebellar hypoplasia, type 16

References:

MONDO:0008566 - thyroid cancer, nonmedullary, 2

References:

GO biological process

GO:0036172 - thiamine salvage

References:

GO cellular component

GO:0030287 - cell wall-bounded periplasmic space

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GO:0005576 - extracellular region

References:

GO:0009277 - fungal-type cell wall

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GO molecular function

GO:0003993 - acid phosphatase activity

References:

GO:0016791 - phosphatase activity

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GO:0042131 - thiamine phosphate phosphatase activity

References:

Modification

MOD:00693 - glycosylated residue

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MOD:00006 - N-glycosylated residue

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MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0006657 - abolished acid phosphatase activity during cellular response to phosphate starvation

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Genotypes:

FYPO:0008351 - abolished AMP 5'-nucleotidase activity

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Genotypes:

FYPO:0008352 - abolished CMP 5'-nucleotidase activity

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Genotypes:

FYPO:0008350 - abolished GMP 5'-nucleotidase activity

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Genotypes:

FYPO:0008353 - abolished UMP 5'-nucleotidase activity

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Genotypes:

FYPO:0008355 - decreased AMP 5'-nucleotidase activity

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Genotypes:

FYPO:0008356 - decreased CMP 5'-nucleotidase activity

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Genotypes:

FYPO:0008357 - decreased GMP 5'-nucleotidase activity

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Genotypes:

FYPO:0008354 - decreased UMP 5'-nucleotidase activity

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Genotypes:

FYPO:0002243 - increased acid phosphatase activity

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Genotypes:

FYPO:0003267 - normal acid phosphatase activity

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0001310 - normal viability in stationary phase

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0001045 - decreased acid phosphatase activity

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0003267 - normal acid phosphatase activity

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00328His_Phos_2His_Pase_clade-2Pfam
cd07061HP_HAP_likeHis_Pase_clade-2CDD
PS00616HIS_ACID_PHOSPHAT_1Acid_Pase_ASPROSITE patterns
PS00778HIS_ACID_PHOSPHAT_2Acid_Pase_ASPROSITE patterns
G3DSA:3.40.50.1240:FF:000119CATH-FunFam
G3DSA:3.40.50.1240His_PPase_superfamCATH-Gene3D
SSF53254His_PPase_superfamSUPERFAMILY
PTHR20963PANTHER
PIRSF000894Acid_phosphataseHistidine_acid_Pase_eukPIRSF
Signal PeptideSignal-Peptide-DeepTMHMM

Orthologs

References / Literature

PMID:1868574 - Thiamine in Schizosaccharomyces pombe: dephosphorylation, intracellular pool, biosynthesis and transport.
Schweingruber AM et al. Curr Genet 1991 Apr;19(4):249-54
PMID:9860839 - Calnexin and BiP interact with acid phosphatase independently of glucose trimming and reglucosylation in Schizosaccharomyces pombe.
Jannatipour M et al. Biochemistry 1998 Dec 08;37(49):17253-61
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:1394510 - Regulation of pho1-encoded acid phosphatase of Schizosaccharomyces pombe by adenine and phosphate.
Schweingruber ME et al. Curr Genet 1992 Oct;22(4):289-92
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:34967420 - Genetic screen for suppression of transcriptional interference reveals fission yeast 14-3-3 protein Rad24 as an antagonist of precocious Pol2 transcription termination.
Garg A et al. Nucleic Acids Res 2022 Jan 25;50(2):803-819
PMID:3536917 - Identification and characterization of thiamin repressible acid phosphatase in yeast.
Schweingruber ME et al. J Biol Chem 1986 Dec 05;261(34):15877-82
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:19933844 - Fission yeast Iec1-ino80-mediated nucleosome eviction regulates nucleotide and phosphate metabolism.
Hogan CJ et al. Mol Cell Biol 2010 Feb;30(3):657-74
PMID:7499352 - Schizosaccharomyces pombe thiamine pyrophosphokinase is encoded by gene tnr3 and is a regulator of thiamine metabolism, phosphate metabolism, mating, and growth.
Fankhauser H et al. J Biol Chem 1995 Nov 24;270(47):28457-62
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:4092931 - Cloning and characterization of two genes restoring acid phosphatase activity in pho1- mutants of Schizosaccharomyces pombe.
Maundrell K et al. Gene 1985;39(2-3):223-30
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:41750377 - Transporter-Driven Glycerophosphocholine (GPC) Toxicity Is Conserved from Fission Yeast to Budding Yeast: Roles for Inositol Pyrophosphates and Gde1 Regulation in Fission Yeast.
Hrach VL et al. Biomolecules 2026 Feb 16;16(2)
PMID:12618370 - Schizosaccharomyces pombe essential genes: a pilot study.
Decottignies A et al. Genome Res 2003 Mar;13(3):399-406
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:39660919 - Efn1 and Efn2 are extracellular 5'-nucleotidases induced during the fission yeast response to phosphate starvation.
Innokentev A et al. mBio 2024 Dec 11;:e0299224
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:2249257 - The structural gene coding for thiamin-repressible acid phosphatase in Schizosaccharomyces pombe.
Yang JW et al. Curr Genet 1990 Oct;18(3):269-72
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs