PomBase home

protein coding gene - alp6 (SPBC428.20c) - gamma tubulin complex GCP3 subunit Alp6

Gene summary

Standard name
alp6
Systematic ID
SPBC428.20c
Product
gamma tubulin complex GCP3 subunit Alp6
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPBC902.01c
UniProt ID
Q9USQ2
ORFeome ID
38/38G01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 480948..483828 reverse strand

Annotation

Complementation

PBO:0091679 - functionally complemented by human GCP3

References:

GO biological process

GO:0031122 - cytoplasmic microtubule organization

References:

GO:0051321 - meiotic cell cycle

References:

GO:0007020 - microtubule nucleation

References:

GO:0090307 - mitotic spindle assembly

References:

GO cellular component

GO:0032153 - cell division site

References:

GO:0005829 - cytosol

References:

GO:0000923 - equatorial microtubule organizing center

References:

GO:0000931 - gamma-tubulin ring complex

References:

GO:0008275 - gamma-tubulin small complex

References:

GO:0061496 - half bridge of mitotic spindle pole body

References:

GO:0061497 - inner plaque of mitotic spindle pole body

References:

GO:0044732 - mitotic spindle pole body

References:

GO:0005634 - nucleus

References:

GO:0071957 - old mitotic spindle pole body

References:

GO molecular function

GO:0051011 - microtubule minus-end binding

References:

GO:0140490 - microtubule nucleator activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00047 - O-phospho-L-threonine

References:

Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0002058 - viable cell population

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000941 - abolished protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0004700 - bent vegetative cell

References:

Genotypes:

FYPO:0003327 - curved interphase microtubules

References:

Genotypes:

FYPO:0000016 - curved vegetative cell

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0002821 - decreased protein localization to mitotic spindle pole body during interphase

References:

Genotypes:

FYPO:0002822 - decreased protein localization to mitotic spindle pole body during mitosis

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0004979 - elongated monoseptate vegetative cell

References:

Genotypes:

FYPO:0004750 - elongated septated vegetative cell

References:

Genotypes:

FYPO:0004950 - elongated vegetative cell with elongated oscillating nucleus

References:

Genotypes:

FYPO:0002379 - inviable after spore germination, without cell division, with elongated germ tube

References:

Genotypes:

FYPO:0002113 - inviable curved vegetative cell

References:

Genotypes:

FYPO:0002066 - inviable elongated aseptate cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000233 - long cytoplasmic microtubules

References:

Genotypes:

FYPO:0004537 - mitotic spindle assembly checkpoint override

References:

Genotypes:

FYPO:0001513 - normal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF04130GCP_C_terminalGCP_CPfam
PF17681GCP_N_terminalGCP_NPfam
G3DSA:1.20.120.1900GCP_C_sfCATH-Gene3D
PTHR19302GCPPANTHER

Orthologs

References / Literature

PMID:16611237 - Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexes.
Masuda H et al. Genes Cells 2006 Apr;11(4):319-36
PMID:15837798 - Efficient formation of bipolar microtubule bundles requires microtubule-bound gamma-tubulin complexes.
Janson ME et al. J Cell Biol 2005 Apr 25;169(2):297-308
PMID:15280226 - Functional dissection of the gamma-tubulin complex by suppressor analysis of gtb1 and alp4 mutations in Schizosaccharomyces pombe.
Tange Y et al. Genetics 2004 Jul;167(3):1095-107
PMID:9658169 - Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants.
Radcliffe P et al. Mol Biol Cell 1998 Jul;9(7):1757-71
PMID:16855399 - An extended anaphase signaling pathway for Mad2p includes microtubule organizing center proteins and multiple motor-dependent transitions.
Mayer C et al. Cell Cycle 2006 Jul;5(13):1456-63
PMID:12134075 - A fourth component of the fission yeast gamma-tubulin complex, Alp16, is required for cytoplasmic microtubule integrity and becomes indispensable when gamma-tubulin function is compromised.
Fujita A et al. Mol Biol Cell 2002 Jul;13(7):2360-73
PMID:12618370 - Schizosaccharomyces pombe essential genes: a pilot study.
Decottignies A et al. Genome Res 2003 Mar;13(3):399-406
PMID:17021256 - Noncore components of the fission yeast gamma-tubulin complex.
Anders A et al. Mol Biol Cell 2006 Dec;17(12):5075-93
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:19942852 - Fission yeast Pcp1 links polo kinase-mediated mitotic entry to gamma-tubulin-dependent spindle formation.
Fong CS et al. EMBO J 2010 Jan 06;29(1):120-30
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11080156 - The fission yeast gamma-tubulin complex is required in G(1) phase and is a component of the spindle assembly checkpoint.
Vardy L et al. EMBO J 2000 Nov 15;19(22):6098-111
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25103238 - Tts1, the fission yeast homologue of the TMEM33 family, functions in NE remodeling during mitosis.
Zhang D et al. Mol Biol Cell 2014 Oct 01;25(19):2970-83
PMID:15004232 - Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function.
Venkatram S et al. Mol Biol Cell 2004 May;15(5):2287-301
PMID:24963130 - The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body.
Wälde S et al. J Cell Sci 2014 Aug 15;127(Pt 16):3625-40
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23886939 - Functional replacement of fission yeast γ-tubulin small complex proteins Alp4 and Alp6 by human GCP2 and GCP3.
Riehlman TD et al. J Cell Sci 2013 Oct 01;126(Pt 19):4406-13
PMID:25987607 - The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies.
Yukawa M et al. J Cell Biol 2015 May 25;209(4):549-62
PMID:23885124 - Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly.
Masuda H et al. Mol Biol Cell 2013 Sep;24(18):2894-906
PMID:37783794 - An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.
Ader NR et al. Nat Cell Biol 2023 Oct;25(10):1465-1477
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21993292 - Microtubule nucleation by γ-tubulin complexes.
Kollman JM et al. Nat Rev Mol Cell Biol 2011 Oct 12;12(11):709-21
PMID:23388459 - Kinesin-14 Pkl1 targets γ-tubulin for release from the γ-tubulin ring complex (γ-TuRC) ‬‬‬‬‬‬‬.
Olmsted ZT et al. Cell Cycle 2013 Mar 01;12(5):842-8
PMID:28619713 - Molecular model of fission yeast centrosome assembly determined by superresolution imaging.
Bestul AJ et al. J Cell Biol 2017 Aug 07;216(8):2409-2424
PMID:15800064 - Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring.
Venkatram S et al. Mol Biol Cell 2005 Jun;16(6):3052-63
PMID:15120067 - Microtubule nucleation at non-spindle pole body microtubule-organizing centers requires fission yeast centrosomin-related protein mod20p.
Sawin KE et al. Curr Biol 2004 May 04;14(9):763-75
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:18418055 - Protein complexes at the microtubule organizing center regulate bipolar spindle assembly.
Rodriguez AS et al. Cell Cycle 2008 May 01;7(9):1246-53
PMID:24006493 - Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3(Alp6).
Dhani DK et al. Mol Biol Cell 2013 Nov;24(21):3337-49
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:35286199 - Mitotic spindle formation in the absence of Polo kinase.
Kim J et al. Proc Natl Acad Sci U S A 2022 Mar 22;119(12):e2114429119