protein coding gene - pre3 (SPBC4C3.10c) - 20S proteasome complex subunit beta 1 Pre3

Gene summary

Standard name: pre3
Systematic ID: SPBC4C3.10c
Product: 20S proteasome complex subunit beta 1 Pre3
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: mts8
UniProt ID: O43063
ORFeome ID: 13/13E12
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome II: 3117646..3118883 forward strand

Annotation

PBO:0000325 - 3.4.25.1

Disease association

MONDO:0968983 - proteasome-associated autoinflammatory syndrome 6

References:

PB_REF:0000006

GO biological process

GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process

References:

GO_REF:0000033

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO:0019774 - proteasome core complex, beta-subunit complex

References:

PMID:21098295

GO molecular function

GO:0004298 - threonine-type endopeptidase activity

References:

GO_REF:0000002

Modification

MOD:00046 - O-phospho-L-serine

References:

PMID:25720772

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

PMID:23697806

Genotypes:

pre3delta

FYPO:0002151 - inviable spore

References:

PMID:23697806

Genotypes:

pre3delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

pre3delta

FYPO:0001387 - loss of viability at high temperature

References:

PMID:23209828

Genotypes:

mts8-1(disruption)

FYPO:0000067 - resistance to brefeldin A

References:

PMID:23209828

Genotypes:

mts8-1(disruption)

FYPO:0000073 - resistance to caffeine

References:

PMID:23209828

Genotypes:

mts8-1(disruption)

FYPO:0004025 - resistance to carbendazim

References:

PMID:23209828

Genotypes:

mts8-1(disruption)

FYPO:0000767 - resistance to staurosporine

References:

PMID:23209828

Genotypes:

mts8-1(disruption)

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF00227	Proteasome	Proteasome_sua/b	PFAM
cd03762	proteasome_beta_type_6		CDD
PS00854	PROTEASOME_BETA_1	Proteasome_bsu_CS	PROSITE_PATTERNS
PS51476	PROTEASOME_BETA_2	Proteasome_suB-type	PROSITE_PROFILES
PR00141	PROTEASOME	Pept_T1A_subB	PRINTS
G3DSA:3.60.20.10:FF:000010			FUNFAM
SSF56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	Ntn_hydrolases_N	SUPERFAMILY
G3DSA:3.60.20.10	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Ntn_hydrolases_N	GENE3D
PTHR32194	METALLOPROTEASE TLDD	Proteasome_suB-type	PANTHER

Orthologs

PSMB6 (HGNC:9543) Homo sapiens
PSMB9 (HGNC:9546) Homo sapiens
pre3 (SJAG_03151) Schizosaccharomyces japonicus
PRE3 (YJL001W) Saccharomyces cerevisiae

References / Literature

PMID:23209828 - Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor.: Penney M et al. PLoS One 2012;7(11):e50796
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:21098295 - Structure of the 26S proteasome from Schizosaccharomyces pombe at subnanometer resolution.: Bohn S et al. Proc Natl Acad Sci U S A 2010 Dec 07;107(49):20992-7
PMID:28784663 - The 19S proteasome is directly involved in the regulation of heterochromatin spreading in fission yeast.: Seo HD et al. J Biol Chem 2017 Oct 13;292(41):17144-17155
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.: Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:20133687 - Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast.: Takeda K et al. Proc Natl Acad Sci U S A 2010 Feb 23;107(8):3540-5
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000002 - Comments
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:20838651 - A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity.: Kouranti I et al. PLoS Biol 2010 Sep 07;8(9)