protein coding gene - dbp8 (SPBC543.06c) - ATP-dependent RNA helicase Dbp8

Gene summary

Standard name: dbp8
Systematic ID: SPBC543.06c
Product: ATP-dependent RNA helicase Dbp8
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: Q9HGM5
ORFeome ID: 33/33B02
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome II: 4310179..4311808 reverse strand

Annotation

GO biological process

GO:0006364 - rRNA processing

References:

GO_REF:0000033

GO cellular component

GO:0005730 - nucleolus

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO molecular function

GO:0005524 - ATP binding

References:

GO_REF:0000002

GO:0003724 - RNA helicase activity

References:

GO_REF:0000024

Modification

MOD:00046 - O-phospho-L-serine

References:

PMID:33823663

Protein features

PBO:0111747 - DEAD/DEAH box helicase

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PMID:39367033

PomGeneEx:0000012 - RNA level decreased

References:

PMID:33313903

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

PMID:23697806

Genotypes:

dbp8delta

FYPO:0002379 - inviable after spore germination, without cell division, with elongated germ tube

References:

PMID:23697806

Genotypes:

dbp8delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

dbp8delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF00271	Helicase_C	Helicase_C-like	PFAM
PF00270	DEAD	DEAD/DEAH_box_helicase_dom	PFAM
cd17955	DEADc_DDX49		CDD
cd18787	SF2_C_DEAD		CDD
PS00039	DEAD_ATP_HELICASE	RNA-helicase_DEAD-box_CS	PROSITE_PATTERNS
PS51195	Q_MOTIF	RNA_helicase_DEAD_Q_motif	PROSITE_PROFILES
PS51192	HELICASE_ATP_BIND_1	Helicase_ATP-bd	PROSITE_PROFILES
PS51194	HELICASE_CTER	Helicase_C-like	PROSITE_PROFILES
SM00490	helicmild6	Helicase_C-like	SMART
SM00487	ultradead3	Helicase_ATP-bd	SMART
G3DSA:3.40.50.300:FF:000993			FUNFAM
SSF52540	P-loop containing nucleoside triphosphate hydrolases	P-loop_NTPase	SUPERFAMILY
G3DSA:3.40.50.300		P-loop_NTPase	GENE3D
PTHR47959	ATP-DEPENDENT RNA HELICASE RHLE-RELATED	DEAD_box_RNA_helicase	PANTHER
mobidb-lite-Disorder	disorder_prediction		MOBIDB-Disorder

Orthologs

DDX49 (HGNC:18684) Homo sapiens
dbp8 (SJAG_01182) Schizosaccharomyces japonicus
DBP8 (YHR169W) Saccharomyces cerevisiae

References / Literature

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.: Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.: Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.: Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
GO_REF:0000002 - Comments
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.: Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399