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protein coding gene - gap1 (SPBC646.12c) - RasGAP Gap1

Gene summary

Standard name
gap1
Systematic ID
SPBC646.12c
Product
RasGAP Gap1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
src1, sar1
UniProt ID
P33277
ORFeome ID
39/39F10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 942688..948187 reverse strand

Annotation

GO biological process

GO:0046580 - negative regulation of Ras protein signal transduction

References:

GO:0031139 - positive regulation of conjugation with cellular fusion

References:

GO:1902917 - positive regulation of mating projection assembly

References:

GO cellular component

GO:0051285 - cell cortex of cell tip

References:

GO:0071944 - cell periphery

References:

GO:0090726 - cortical dynamic polarity patch

References:

GO:0005829 - cytosol

References:

GO:0000935 - division septum

References:

GO:0005937 - mating projection

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005096 - GTPase activator activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0006503 - abnormal protein localization to actin fusion focus

References:

Genotypes:

FYPO:0006505 - abolished protein localization to shmoo tip

References:

Genotypes:

FYPO:0000303 - decreased conjugation frequency

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0001871 - increased agglutination

References:

Genotypes:

FYPO:0001219 - increased protein level during cellular response to nitrogen starvation

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Genotypes:

FYPO:0004581 - increased transcriptional response to pheromone

References:

Genotypes:

FYPO:0000760 - normal mating

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Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0006500 - premature cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0000022 - shmoo with long tip

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Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0006540 - abnormal actin cytoskeleton organization during cellular response to pheromone

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

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Genotypes:

FYPO:0000303 - decreased conjugation frequency

References:

Genotypes:

FYPO:0001531 - decreased GTPase activity

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0003770 - decreased transcriptional response to pheromone

References:

Genotypes:

FYPO:0000998 - elongated cell during nitrogen starvation

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Genotypes:

FYPO:0001871 - increased agglutination

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0009076 - increased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0001219 - increased protein level during cellular response to nitrogen starvation

References:

Genotypes:

FYPO:0006537 - loss of viability following cellular response to pheromone

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0006504 - multiple shmoo formation

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Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0000997 - pear-shaped cell during nitrogen starvation

References:

Genotypes:

FYPO:0006501 - premature actin fusion focus assembly

References:

Genotypes:

FYPO:0006500 - premature cell wall disassembly at cell fusion site

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Genotypes:

FYPO:0006386 - protein abnormally dispersed at shmoo tip

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Genotypes:

FYPO:0004730 - protein mislocalized to lateral cell cortex

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

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Genotypes:

FYPO:0002634 - resistance to cobalt

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009039 - resistance to potassium chloride

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Genotypes:

FYPO:0005968 - resistance to sodium chloride

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Genotypes:

FYPO:0009089 - resistance to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0002546 - sensitive to trichostatin A

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

References:

Genotypes:

FYPO:0006011 - shmoo formation at cell side

References:

Genotypes:

FYPO:0000022 - shmoo with long tip

References:

Genotypes:

FYPO:0000280 - sterile

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF00616RasGAPRasGAP_domPfam
PF03836IQGAP_helicalIQGAP_helicalPfam
PF29130IQGAP_CIQGAP_CPfam
cd05132RasGAP_GAPACDD
PS00509RAS_GTPASE_ACTIV_1RasGAP_CSPROSITE patterns
PS50018RAS_GTPASE_ACTIV_2RasGAP_domPROSITE profiles
SM00323RasGAPRasGAP_domSMART
G3DSA:1.10.506.10Rho_GTPase_activation_protCATH-Gene3D
SSF143885SUPERFAMILY
SSF48350Rho_GTPase_activation_protSUPERFAMILY
PTHR14149PANTHER

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24147005 - The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis.
Weston C et al. PLoS One 2013;8(10):e77487
PMID:2038319 - Identification of a GTPase-activating protein homolog in Schizosaccharomyces pombe.
Imai Y et al. Mol Cell Biol 1991 Jun;11(6):3088-94
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:10022880 - Control of growth and differentiation by Drosophila RasGAP, a homolog of p120 Ras-GTPase-activating protein.
Feldmann P et al. Mol Cell Biol 1999 Mar;19(3):1928-37
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:1883874 - sar1, a gene from Schizosaccharomyces pombe encoding a protein that regulates ras1.
Wang Y et al. Cell Regul 1991 Jun;2(6):453-65
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:29453312 - Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.
Merlini L et al. J Cell Biol 2018 Apr 02;217(4):1467-1483
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:9133664 - The Schizosaccharomyces pombe mra1 gene, which is required for cell growth and mating, can suppress the mating inefficiency caused by a deficit in the Ras1 activity.
Hakuno F et al. Genes Cells 1996 Mar;1(3):303-15
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:27020743 - Local Pheromone Release from Dynamic Polarity Sites Underlies Cell-Cell Pairing during Yeast Mating.
Merlini L et al. Curr Biol 2016 Apr 25;26(8):1117-25
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87