PomBase home

protein coding gene - orc5 (SPBC646.14c) - origin recognition complex subunit Orc5

Gene summary

Standard name
orc5
Systematic ID
SPBC646.14c
Product
origin recognition complex subunit Orc5
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O43114
ORFeome ID
40/40E10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 952334..954317 reverse strand

Annotation

GO biological process

GO:1902975 - mitotic DNA replication initiation

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0031261 - DNA replication preinitiation complex

References:

GO:0005664 - nuclear origin of replication recognition complex

References:

GO:0005656 - nuclear pre-replicative complex

References:

GO:0043596 - nuclear replication fork

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0003688 - DNA replication origin binding

References:

GO:0005515 - protein binding

References:

Modification

MOD:00047 - O-phospho-L-threonine

References:

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0001270 - complete but unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0001052 - cut, small cell

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003004 - increased cellular reactive oxygen species level during vegetative growth

References:

Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0000416 - premature mitotic sister chromatid separation

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Protein features

PBO:0111792 - AAA family ATPase

Qualitative gene expression

PomGeneEx:0000020 - protein level unchanged

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000400 - abnormal cell cycle arrest at mitotic G2/M phase transition

References:

Genotypes:

FYPO:0000611 - abnormal cell cycle arrest in mitotic S phase

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0001343 - abnormal mitotic DNA replication

References:

Genotypes:

FYPO:0000670 - abnormal mitotic sister chromatid separation

References:

Genotypes:

FYPO:0003548 - abolished mitotic G1/S phase transition following nitrogen starvation-induced G1 phase arrest

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0005167 - decreased protein localization to chromatin at centromere inner repeat

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003004 - increased cellular reactive oxygen species level during vegetative growth

References:

Genotypes:

FYPO:0000377 - increased frequency of apoptosis

References:

Genotypes:

FYPO:0000786 - increased plasmid loss

References:

Genotypes:

FYPO:0003760 - inviable after spore germination, single or double cell division, arrest with cut

References:

Genotypes:

FYPO:0002724 - inviable after spore germination, single or double cell division, elongated cell

References:

Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0005367 - inviable mononucleate aseptate cell with cell cycle arrest at mitotic G2/M phase transition

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0001790 - normal plasmid loss

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF13191AAA_16AAA_16PFAM
PF14630ORC5_CORC5_CPFAM
PF21639ORC5_lidORC5_lidPFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR12705ORIGIN RECOGNITION COMPLEX SUBUNIT 5ORC5PANTHER

Orthologs

References / Literature

PMID:18723846 - Multiple mechanisms contribute to Schizosaccharomyces pombe origin recognition complex-DNA interactions.
Houchens CR et al. J Biol Chem 2008 Oct 31;283(44):30216-24
PMID:22645654 - Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.
Akai Y et al. Open Biol 2011 Dec;1(4):110023
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:11850415 - Purification and characterization of the Schizosaccharomyces pombe origin recognition complex: interaction with origin DNA and Cdc18 protein.
Chuang RY et al. J Biol Chem 2002 May 10;277(19):16920-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000051 - S. pombe keyword mapping
PMID:11683912 - Interaction of fission yeast ORC with essential adenine/thymine stretches in replication origins.
Takahashi T et al. Genes Cells 2001 Oct;6(10):837-49
PMID:11717425 - The Schizosaccharomyces pombe origin recognition complex interacts with multiple AT-rich regions of the replication origin DNA by means of the AT-hook domains of the spOrc4 protein.
Lee JK et al. Proc Natl Acad Sci U S A 2001 Nov 20;98(24):13589-94
PMID:12185500 - Fission yeast Cdc23 interactions with DNA replication initiation proteins.
Hart EA et al. Curr Genet 2002 Aug;41(5):342-8
PMID:18414064 - Schizosaccharomyces pombe Orc5 plays multiple roles in the maintenance of genome stability throughout the cell cycle.
Kato H et al. Cell Cycle 2008 Apr 15;7(8):1085-96
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:10535928 - Identification and reconstitution of the origin recognition complex from Schizosaccharomyces pombe.
Moon KY et al. Proc Natl Acad Sci U S A 1999 Oct 26;96(22):12367-72
PMID:10523506 - The fission yeast origin recognition complex is constitutively associated with chromatin and is differentially modified through the cell cycle.
Lygerou Z et al. J Cell Sci 1999 Nov;112 ( Pt 21):3703-12
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.
Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119
PMID:11689699 - Site-specific DNA binding of the Schizosaccharomyces pombe origin recognition complex is determined by the Orc4 subunit.
Kong D et al. Mol Cell Biol 2001 Dec;21(23):8095-103
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:16371652 - Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast.
Marchetti MA et al. J Cell Sci 2006 Jan 01;119(Pt 1):124-31
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.
Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80