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protein coding gene - dot2 (SPBC651.05c) - ESCRT II complex subunit Dot2

Gene summary

Standard name
dot2
Systematic ID
SPBC651.05c
Product
ESCRT II complex subunit Dot2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
vps22
UniProt ID
O94663
ORFeome ID
10/10E05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1245920..1247077 reverse strand

Annotation

Disease association

MONDO:0968946 - developmental and epileptic encephalopathy 115

References:

MONDO:0968947 - neurodevelopmental disorder plus optic atrophy

References:

GO biological process

GO:0045324 - late endosome to vacuole transport

References:

GO:0043328 - protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

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GO cellular component

GO:0005829 - cytosol

References:

GO:0000814 - ESCRT II complex

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GO:0016604 - nuclear body

References:

GO:0005654 - nucleoplasm

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GO:0005634 - nucleus

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GO:0035974 - meiotic spindle pole body

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Modification

MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0004609 - spindle pole bodies present in increased numbers during meiosis

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001001 - abnormal cell cycle arrest at mitotic G2/M phase transition during nitrogen starvation

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Genotypes:

FYPO:0003287 - abnormal cell cycle arrest in meiotic interphase before premeiotic DNA replication

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Genotypes:

FYPO:0000197 - abnormal horsetail movement

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Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0000734 - abnormal meiotic spindle

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Genotypes:

FYPO:0000172 - abnormal meiotic telomere clustering

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Genotypes:

FYPO:0003549 - abnormal protein localization during meiosis

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Genotypes:

FYPO:0006022 - abnormal spindle pole body morphology during meiosis I

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Genotypes:

FYPO:0004608 - abnormal spindle pole body morphology during meiosis II

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Genotypes:

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0004482 - abnormal vacuole fusion during vegetative growth

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Genotypes:

FYPO:0000511 - abolished nuclear fusion during mating

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Genotypes:

FYPO:0000583 - abolished sporulation

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0004628 - delayed onset of premeiotic DNA replication

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Genotypes:

FYPO:0003611 - increased protein level during meiosis

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Genotypes:

FYPO:0001460 - increased transcription from CDRE promoter in response to calcium ion

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0005961 - multipolar meiotic spindle

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Genotypes:

FYPO:0004295 - multiseptate cell

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Genotypes:

FYPO:0002043 - normal premeiotic DNA replication

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Genotypes:

FYPO:0001986 - resistance to 5-fluorouracil

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Genotypes:

FYPO:0006580 - resistance to amiodarone

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

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Genotypes:

FYPO:0005968 - resistance to sodium chloride

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Genotypes:

FYPO:0005253 - resistance to tamoxifen

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Genotypes:

FYPO:0005193 - resistance to torin1

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Genotypes:

FYPO:0002642 - sensitive to amphotericin B

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0000101 - sensitive to chlorpropham

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Genotypes:

FYPO:0002640 - sensitive to clotrimazole

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0006579 - sensitive to manganese

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000751 - sensitive to nickel

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0004609 - spindle pole bodies present in increased numbers during meiosis

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04157EAP30Snf8/Vps36Pfam
G3DSA:1.10.10.10:FF:000397CATH-FunFam
G3DSA:1.10.10.10WH-like_DNA-bd_sfCATH-Gene3D
G3DSA:6.10.140.180CATH-Gene3D
SSF46785WH_DNA-bd_sfSUPERFAMILY
PTHR12806ESCRT-2_cplx_Snf8PANTHER
PIRSF017215ESCRT2_Vps22ESCRT-2_cplx_Snf8PIRSF

Orthologs

References / Literature

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:17660439 - Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe.
Iwaki T et al. Microbiology (Reading) 2007 Aug;153(Pt 8):2753-2764
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:15992541 - The fission yeast homolog of the human transcription factor EAP30 blocks meiotic spindle pole body amplification.
Jin Y et al. Dev Cell 2005 Jul;9(1):63-73
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:26628015 - Characterization of Tamoxifen as an Antifungal Agent Using the Yeast Schizosaccharomyces Pombe Model Organism.
Zhang X et al. Kobe J Med Sci 2015 Oct 09;61(2):E54-63
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:11901107 - Fission yeast mutants affecting telomere clustering and meiosis-specific spindle pole body integrity.
Jin Y et al. Genetics 2002 Mar;160(3):861-76
PMID:19669754 - Enhanced protein secretion from multiprotease-deficient fission yeast by modification of its vacuolar protein sorting pathway.
Idiris A et al. Appl Microbiol Biotechnol 2010 Jan;85(3):667-77
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:29856841 - Genome-wide screen reveals important roles for ESCRT proteins in drug/ion resistance of fission yeast.
Yang Y et al. PLoS One 2018;13(6):e0198516
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323