PomBase home

protein coding gene - dot2 (SPBC651.05c) - ESCRT II complex subunit Dot2

Gene summary

Standard name
dot2
Systematic ID
SPBC651.05c
Product
ESCRT II complex subunit Dot2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
vps22
UniProt ID
O94663
ORFeome ID
10/10E05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1245920..1247077 reverse strand

Annotation

Disease association

MONDO:0968946 - developmental and epileptic encephalopathy 115

References:

MONDO:0968947 - neurodevelopmental disorder plus optic atrophy

References:

GO biological process

GO:0045324 - late endosome to vacuole transport

References:

GO:0043328 - protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0000814 - ESCRT II complex

References:

GO:0016604 - nuclear body

References:

GO:0005654 - nucleoplasm

References:

GO:0005634 - nucleus

References:

GO:0035974 - meiotic spindle pole body

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0004609 - spindle pole bodies present in increased numbers during meiosis

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001001 - abnormal cell cycle arrest at mitotic G2/M phase transition during nitrogen starvation

References:

Genotypes:

FYPO:0003287 - abnormal cell cycle arrest in meiotic interphase before premeiotic DNA replication

References:

Genotypes:

FYPO:0000197 - abnormal horsetail movement

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0000734 - abnormal meiotic spindle

References:

Genotypes:

FYPO:0000172 - abnormal meiotic telomere clustering

References:

Genotypes:

FYPO:0003549 - abnormal protein localization during meiosis

References:

Genotypes:

FYPO:0006022 - abnormal spindle pole body morphology during meiosis I

References:

Genotypes:

FYPO:0004608 - abnormal spindle pole body morphology during meiosis II

References:

Genotypes:

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0004482 - abnormal vacuole fusion during vegetative growth

References:

Genotypes:

FYPO:0000511 - abolished nuclear fusion during mating

References:

Genotypes:

FYPO:0000583 - abolished sporulation

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0004628 - delayed onset of premeiotic DNA replication

References:

Genotypes:

FYPO:0003611 - increased protein level during meiosis

References:

Genotypes:

FYPO:0001460 - increased transcription from CDRE promoter in response to calcium ion

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0005961 - multipolar meiotic spindle

References:

Genotypes:

FYPO:0004295 - multiseptate cell

References:

Genotypes:

FYPO:0002043 - normal premeiotic DNA replication

References:

Genotypes:

FYPO:0001986 - resistance to 5-fluorouracil

References:

Genotypes:

FYPO:0006580 - resistance to amiodarone

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0005969 - resistance to magnesium chloride

References:

Genotypes:

FYPO:0005968 - resistance to sodium chloride

References:

Genotypes:

FYPO:0005253 - resistance to tamoxifen

References:

Genotypes:

FYPO:0005193 - resistance to torin1

References:

Genotypes:

FYPO:0002642 - sensitive to amphotericin B

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000098 - sensitive to calcium

References:

Genotypes:

FYPO:0000101 - sensitive to chlorpropham

References:

Genotypes:

FYPO:0002640 - sensitive to clotrimazole

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006579 - sensitive to manganese

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000751 - sensitive to nickel

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0004609 - spindle pole bodies present in increased numbers during meiosis

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04157EAP30Snf8/Vps36PFAM
G3DSA:1.10.10.10:FF:000397FUNFAM
SSF46785"Winged helix" DNA-binding domainWH_DNA-bd_sfSUPERFAMILY
G3DSA:6.10.140.180GENE3D
G3DSA:1.10.10.10WH-like_DNA-bd_sfGENE3D
PTHR12806EAP30 SUBUNIT OF ELL COMPLEXESCRT-2_cplx_Snf8PANTHER
PIRSF017215ESCRT2_Vps22ESCRT-2_cplx_Snf8PIRSF

Orthologs

References / Literature

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:26628015 - Characterization of Tamoxifen as an Antifungal Agent Using the Yeast Schizosaccharomyces Pombe Model Organism.
Zhang X et al. Kobe J Med Sci 2015 Oct 09;61(2):E54-63
PMID:19669754 - Enhanced protein secretion from multiprotease-deficient fission yeast by modification of its vacuolar protein sorting pathway.
Idiris A et al. Appl Microbiol Biotechnol 2010 Jan;85(3):667-77
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:11901107 - Fission yeast mutants affecting telomere clustering and meiosis-specific spindle pole body integrity.
Jin Y et al. Genetics 2002 Mar;160(3):861-76
PMID:15992541 - The fission yeast homolog of the human transcription factor EAP30 blocks meiotic spindle pole body amplification.
Jin Y et al. Dev Cell 2005 Jul;9(1):63-73
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:29856841 - Genome-wide screen reveals important roles for ESCRT proteins in drug/ion resistance of fission yeast.
Yang Y et al. PLoS One 2018;13(6):e0198516
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:17660439 - Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe.
Iwaki T et al. Microbiology (Reading) 2007 Aug;153(Pt 8):2753-2764
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053